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Influence of minor displacements in loops of the porcine parvovirus VP2 capsid on virus-like particles assembly and the induction of antibody responses

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Abstract

An antigen-delivery system based on hybrid virus-like particles (VLPs) formed by the self-assembly of the capsid VP2 protein of porcine parvovirus (PPV) and expressing foreign peptides offers an alternative method for vaccination. In this study, the three-dimensional structure of the PPV capsid protein and surface loops deletion mutants were analyzed to define essential domains in PPV VP2 for the assembly of VLPs. Electron microscopic analysis and SDS-PAGE analysis confirmed the presence of abundant VLPs in a loop2 deletion mutant of expected size and appropriate morphology. Loop4 and loop2–loop4 deletion mutants, however, resulted in a lower number of particles and the morphology of the particles was not well preserved. Furthermore, the green fluorescent protein (gfp) gene was used as a model. GFP was observed at the same level in displacements mutants. However, GFP displacement mutants in loop2 construct allowed better adaptation for the fusion GFP to be further displayed on the surface of the capsid-like structure. Immunogenicity study showed that there is no obvious difference in mice inoculated with rAd-VP2(Δloop2), rAd-VP2(Δloop4), rAd-VP2(Δloop2–Δloop4), and PPV inactivated vaccine. The results suggested the possibility of inserting simultaneously B and T cell epitopes in the surface loop2 and the N-terminus. The combination of different types of epitopes (B, CD4+, and CD8+) in different positions of the PPV particles opens the way to the development of highly efficient vaccines, able to stimulate at the same time the different branches of the immune system.

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Acknowledgments

The project was supported by the State Natural Sciences Foundation project of China (31001055).

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Authors and Affiliations

  1. Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology Ministry of Agriculture, National Center for Engineering Research of Veterinary Bio-products, Nanjing, 210014, Jiangsu Province, China

    Qunxing Pan, Kongwang He, Yongshan Wang, Xiaoli Wang & Wei Ouyang

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  1. Qunxing Pan
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  2. Kongwang He
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  3. Yongshan Wang
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  4. Xiaoli Wang
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  5. Wei Ouyang
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Correspondence to Qunxing Pan.

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Pan, Q., He, K., Wang, Y. et al. Influence of minor displacements in loops of the porcine parvovirus VP2 capsid on virus-like particles assembly and the induction of antibody responses. Virus Genes 46, 465–472 (2013). https://doi.org/10.1007/s11262-013-0891-5

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  • DOI: https://doi.org/10.1007/s11262-013-0891-5

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