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A betasatellite-dependent begomovirus infects ornamental rose: characterization of begomovirus infecting rose in Pakistan

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Abstract

The Begomovirus genus of the family Geminiviridae comprises the largest group of geminiviruses. The list of begomoviruses is continuously increasing as a result of improvement in the methods for identification. Ornamental rose plants (Rosa chinensis) with highly stunted growth and leaf curling were found in Faisalabad, Pakistan. Plants were analyzed for begomovirus infection, through rolling circle amplification and PCR methods. Based on complete genome sequence homologies with other begomoviruses, a new begomovirus species infecting the rose plants was discovered. In this paper, we propose a new species name, Rose leaf curl virus (RoLCuV), for the virus. RoLCuV showed close identity (83 %) with Tomato leaf curl Pakistan virus, while associated betasatellite showed 96 % identity with Digera arvensis yellow vein betasatellite (DiAYVB), justifying a new isolate for the betasatellite. Recombination analysis of newly identified begomovirus revealed it as a recombinant of tomato leaf curl Pakistan virus from its coat protein region. The infectious molecules for virus/satellite were prepared and inoculated through Agrobacterium tumefaciens to N. benthamiana plants. RoLCuV alone was unable to induce any level of symptoms on N. benthamiana plants, but co-inoculation with cognate betasatellite produced infection symptoms. Further investigation to understand the trans-replication ability of betasatellites revealed their flexibility to interact with Rose leaf curl virus.

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Acknowledgments

This project was supported by the Danforth Plant Science Center. Higher Education Commission of Pakistan supported NN under a six-month fellowship in ILTAB at the Danforth Center. MM is supported by IFS grant no C/5260-1. The authors are thankful to the greenhouse staff at the Danforth Center for maintaining plants.

Conflict of interest

The authors declare that they have no competing interests.

Author information

Author notes

  1. Sandeep Khatri

    Present address: Department of Human Genetics, University of Pittsburgh, Pittsburgh, PA, 15261, USA

  2. Nazia Nahid

    Present address: Department of Bioinformatics and Biotechnology, Government College University, Faisalabad, 38000, Pakistan

Authors and Affiliations

  1. ILTAB, Donald Danforth Plant Science Center, 975 North Warson Road, Saint Louis, MO, 63132, USA

    Sandeep Khatri, Nazia Nahid & Claude M. Fauquet

  2. Virology Lab, Center for Agricultural Biochemistry and Biotechnology, University of Agriculture, Faisalabad, 38040, Pakistan

    Muhammad Mubin & Muhammad Shah Nawaz-ul-Rehman

Authors
  1. Sandeep Khatri
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  2. Nazia Nahid
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  3. Claude M. Fauquet
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  4. Muhammad Mubin
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  5. Muhammad Shah Nawaz-ul-Rehman
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Corresponding author

Correspondence to Muhammad Shah Nawaz-ul-Rehman.

Additional information

Sandeep Khatri and Nazia Nahid have contributed equally.

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Khatri, S., Nahid, N., Fauquet, C.M. et al. A betasatellite-dependent begomovirus infects ornamental rose: characterization of begomovirus infecting rose in Pakistan. Virus Genes 49, 124–131 (2014). https://doi.org/10.1007/s11262-014-1076-6

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  • DOI: https://doi.org/10.1007/s11262-014-1076-6

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