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VP6 gene diversity in 11 Brazilian strains of porcine group C rotavirus

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Abstract

Porcine group C rotavirus (RVC) is recognised as an enteric pathogen in piglets worldwide. The VP6 gene of RVC is divided into seven I-genotypes. Genotypes I2 and I3 are found in human and bovine strains, respectively; the porcine strains are divided into the other five genotypes (I1, I4–I7). In this study, molecular analysis of nearly the full length of the VP6 gene was performed in 11 Brazilian wild-type porcine RVC strains identified in diarrhoeic faecal samples, which were collected from eight pig farms located in five Brazilian states from piglets of 1–4 weeks of age. The nucleotide sequences of the VP6 gene showed 82.9–100 % identity between the Brazilian strains, 84.9–93.1 % with the prototype Cowden strain, and 82.4–92.2 % with other porcine RVC strains. In the 11 diarrhoeic faecal samples analysed in this study, three distinct porcine RVC genotypes (I1, I5, and I6) were identified and none were predominant. The results presented in this study revealed a high nucleotide diversity of the VP6 gene in porcine RVC field strains circulating in Brazil, which highlights the importance of further epidemiological and molecular surveys worldwide.

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Acknowledgments

This work was supported by Brazilian institutes: the National Counsel of Scientific and Technological Development (CNPq), the Brazilian Federal Agency for Support and Evaluation of Graduate Education (CAPES), Financing of Studies and Projects (FINEP), and the Araucária Foundation (FAP/PR). Lorenzetti, E., Alfieri, A.F., and Alfieri, A.A. are recipients of CNPq fellowships.

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  1. Danilo Tancler Stipp

    Present address: Laboratory of Veterinary Preventive Medicine, Department of Veterinary Science, Universidade Federal da Paraíba, Areia, Paraíba, Brazil

Authors and Affiliations

  1. Laboratory of Animal Virology, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid - Campus Universitário, PO Box 10011, Londrina, Paraná, CEP 86057-970, Brazil

    Danilo Tancler Stipp, Alice Fernandes Alfieri, Elis Lorenzetti, Thais Neris da Silva Medeiros, Flávia Possatti & Amauri Alcindo Alfieri

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  1. Danilo Tancler Stipp
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Correspondence to Amauri Alcindo Alfieri.

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Stipp, D.T., Alfieri, A.F., Lorenzetti, E. et al. VP6 gene diversity in 11 Brazilian strains of porcine group C rotavirus. Virus Genes 50, 142–146 (2015). https://doi.org/10.1007/s11262-014-1133-1

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