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Two proteins of Cucurbit chlorotic yellows virus, P59 and P9, are self-interacting

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Abstract

The yeast two-hybrid (Y2H) assay, a powerful tool for identifying protein–protein interactions, has been widely used to study viral protein interactions and to elucidate the functions of viral proteins. In this study, Cucurbit chlorotic yellows virus-encoded proteins were investigated by Y2H assays in all possible pairwise combinations, and the self-interactions of P59 and P9 were detected. The interacting domains of P59 and P9 were identified using vectors carrying an activation domain fused to a truncated version of P59 or P9. We found that the middle region (amino acids 173–344) of P59 was necessary for this self-interaction, while three different truncated versions of P9 showed no interaction with full-length P9. This is the first report of the self-interaction of P59 in the genus Crinivirus.

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Acknowledgments

Financial support was provided by the Joint Funds of National Nature Foundation of China (U12041968) and Postdoctoral Science Foundation of China (2013M530338).

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Authors and Affiliations

  1. College of Plant Protection, Henan Agricultural University, Zhengzhou, 450002, China

    Zhenyue Wang, Yingzhi Wang, Honglian Li, Bingjian Sun, Yajuan Shi & Yan Shi

  2. Plant Protection Institute, Henan Academy of Agricultural Sciences, Zhengzhou, 450002, China

    Hu Sun

  3. Zhengzhou Fruit Research Institute, Chinese Academy of Agriculture Sciences, Zhengzhou, 450009, China

    Qinsheng Gu

Authors
  1. Zhenyue Wang
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  2. Yingzhi Wang
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  3. Hu Sun
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  4. Qinsheng Gu
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  5. Honglian Li
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  6. Bingjian Sun
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  7. Yajuan Shi
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  8. Yan Shi
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Correspondence to Hu Sun or Yan Shi.

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Edited by Thomas Hohn.

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Wang, Z., Wang, Y., Sun, H. et al. Two proteins of Cucurbit chlorotic yellows virus, P59 and P9, are self-interacting. Virus Genes 51, 152–155 (2015). https://doi.org/10.1007/s11262-015-1203-z

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  • DOI: https://doi.org/10.1007/s11262-015-1203-z

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