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A live gI/gE-deleted pseudorabies virus (PRV) protects weaned piglets against lethal variant PRV challenge

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Abstract

Emerging pseudorabies virus (PRV) variant has led to frequent outbreaks of PRV infection among Bartha-K61-vaccinated swine population in Chinese swine farms and caused high mortality in pigs of all age since late 2011. Here, we generated a gE/gI-deleted PRV (rPRVXJ-delgI/gE-EGFP) based on PRV variant strain (PRV-XJ) through homologous DNA recombination. Compared to parental strain, rPRVXJ-delgI/gE-EGFP showed similar growth kinetics in vitro. Its safety and immunogenicity were evaluated in weaned piglets. Our results showed that piglets immunized with rPRVXJ-delgI/gE-EGFP did not exhibit any clinical symptoms, and a high level of gB-specific antibody was detected. After lethal challenge with variant PRV (PRV-FJ strain), all vaccinated piglets survived without showing any clinical symptoms except slight fever within 7 days post-challenge. In unvaccinated piglets, typical clinical symptoms of pseudorabies were observed, and the piglets were all died at 5 days post-challenge. These results indicated that a live rPRVXJ-delgI/gE-EGFP vaccine could be a maker vaccine candidate to control the currently epidemic pseudorabies in China.

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Acknowledgements

This work were supported by the Science and Technology program of Sichuan (Project Nos. 2014NZ0043 and 2017NZ0038), Key Projects in the National Science & Technology Pillar Program during the Twelfth Five-year Plan Period (2015BAD12B04-2.3) and Sichuan Crops and Animals Breeding Special Project during the Thirteenth Five-year Plan Period (2016NYZ0052).

Author contributions

Conceived and designed the experiments: Xiaowan Liu, Yue Yin, Zhiwen Xu. Performed the experiments: Xiaowan Liu, Yue Yin. Analyzed the data: Ping Li, Fan Yang. Contributed reagents/materials/analysis tools: Ping Li, Fan Yang, Yi Fan, Xiangang Sun. Wrote the paper: Yue Yin. All the authors had read and approved the article.

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Author notes

  1. Yue Yin, Zhiwen Xu, and Xiaowan Liu contributed equally to this work and share co-first authorship.

Authors and Affiliations

  1. College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, China

    Yue Yin, Zhiwen Xu, Xiaowan Liu, Ping Li, Fan Yang, Jun Zhao, Yi Fan, Xiangang Sun & Ling Zhu

  2. Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, Sichuan, China

    Zhiwen Xu & Ling Zhu

Authors
  1. Yue Yin
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  2. Zhiwen Xu
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  3. Xiaowan Liu
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  4. Ping Li
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  5. Fan Yang
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  6. Jun Zhao
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  7. Yi Fan
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  8. Xiangang Sun
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  9. Ling Zhu
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Corresponding author

Correspondence to Ling Zhu.

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All authors in this paper declare they have no conflict of interest.

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All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

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Edited by Keizo Tomonaga.

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Yin, Y., Xu, Z., Liu, X. et al. A live gI/gE-deleted pseudorabies virus (PRV) protects weaned piglets against lethal variant PRV challenge. Virus Genes 53, 565–572 (2017). https://doi.org/10.1007/s11262-017-1454-y

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  • DOI: https://doi.org/10.1007/s11262-017-1454-y

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