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Genomic analysis of a recombinant NADC30-like porcine reproductive and respiratory syndrome virus in china

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Abstract

Recently, NADC30-like porcine reproductive and respiratory syndrome viruses (PRRSVs), which are genetically similar to the NADC30 strain isolated in the United States of America in 2008, have become prevalent in China. Here, a novel variant PRRSV strain named HNhx was successfully isolated on porcine alveolar macrophages from Henan province and the full-length genome sequence was determined. Phylogenetic analysis indicated that HNhx strain was classified into the NADC30-like PRRSV subgroup, in which all the strains had the unique discontinuous 131-amino acid deletion relative to that of the nonstructural protein 2 (Nsp2) of the VR2332 strain. Genetically, HNhx shared 92.9% nucleotide similarity to NADC30. Furthermore, HNhx strain contained extensive amino acid mutations in GP5. In particular, the S32H, N33D, D34N, and S36G variations resulted in that HNhx lost all the putative N-linked glycosylation sites at amino acid positions 30, 32, 33, 34, and 35. Recombination analysis revealed that HNhx was the result of recombination between the NADC30 strain and the highly pathogenic PRRSV vaccine strain circulating in China in Nsp4 (nt 5261) to Nsp9 (nt 7911). The novel genome data of HNhx will be helpful for understanding the evolution and epidemiology of PRRSV in China.

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Acknowledgements

This study was supported by grants from National Natural Science Funds (31502043, 31502040), the key project of National Natural Science Foundation of China (31490601), China Agriculture Research System (No. CARS-36), the Pig Industry Technology System Innovation Team Project of Henan Province (S2012-06).

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  1. Lin-jian Wang and Bo Wan contributed equally to this work.

Authors and Affiliations

  1. Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, 450002, China

    Lin-jian Wang, Zhenhua Guo, Songlin Qiao, Rui Li, Sha Xie, Xin-xin Chen & Gaiping Zhang

  2. College of Life Sciences, Henan Agricultural University, Zhengzhou, 450002, China

    Lin-jian Wang & Gaiping Zhang

  3. College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450002, China

    Bo Wan & Gaiping Zhang

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  1. Lin-jian Wang
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Contributions

Lin-jian Wang, Xin-xin Chen, and Gaiping Zhang conceived and designed the experiments. Lin-jian Wang, Bo Wan, and Zhenhua Guo performed the experiments. Bo Wan, Songlin Qiao, Rui Li, and Sha Xie contributed data analysis. Lin-jian Wang and Xin-xin Chen wrote the manuscript. Zhenhua Guo, Songlin Qiao, Rui Li, Sha Xie, Gaiping Zhang revised the manuscipt content. Gaiping Zhang, Songlin Qiao, and Xin-xin Chen approved final version of manuscript.

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Correspondence to Xin-xin Chen or Gaiping Zhang.

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All authors state that they have no conflicts of interest. This article does not contain any studies with animals performed by any of the authors. Informed consent was obtained from all individual participants included in the study.

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Wang, Lj., Wan, B., Guo, Z. et al. Genomic analysis of a recombinant NADC30-like porcine reproductive and respiratory syndrome virus in china. Virus Genes 54, 86–97 (2018). https://doi.org/10.1007/s11262-017-1516-1

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