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Utility of high-throughput DNA sequencing in the study of the human papillomaviruses

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Abstract

The Papillomaviridae family is probably the most diverse group of viruses that affect vertebrates. The study of the relationship between infection by certain types of human papillomavirus (HPV) and the development of neoplastic epithelial lesions is of particular interest because of the high prevalence of HPV-related carcinomas in populations of developing countries. To understand the mechanisms of infection and their association with different clinical manifestations, molecular tools play an important role in the description of new types of HPV, the characterization of effector properties of the viral factors, the specific diagnosis and monitoring of HPV types, and the alteration patterns at genetic level in the host. Technological advances in the field of DNA sequencing have led to the development of different next-generation sequencing systems, allowing obtaining a large amount of data and broadening the applications to study viral diseases. In this review, we summarize the main approaches and their perspectives where the use of massively parallel sequencing has been proved as a useful tool in the research of the HPV infection.

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Acknowledgements

G.C.E. received support from Estímulos al Desempeño en Investigación, Comisión y Fomento de Actividades Académicas (Instituto Politécnico Nacional), and Sistema Nacional de Investigadores (SNI, CONACyT). This study was funded by Secretaría de Investigación y Posgrado del Instituto Politécnico Nacional (SIP 20160609 and 20171099). The authors are grateful to Sofia Mulia for kindly translating and correcting the style of the manuscript.

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  1. Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Mexico City, Mexico

    Noé Escobar-Escamilla & Graciela Castro-Escarpulli

  2. Instituto de Diagnóstico y Referencia Epidemiológicos (InDRE) “Dr. Manuel Martínez Báez”, Secretaría de Salud, Mexico City, Mexico

    Noé Escobar-Escamilla, José Ernesto Ramírez-González & José Alberto Díaz-Quiñonez

  3. División de Estudios de Posgrado, Facultad de Medicina, Universidad Nacional Autónoma de México, Mexico City, Mexico

    José Alberto Díaz-Quiñonez

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NEE developed the structural design of the review and drafted the manuscript. GCE, JERG, and JADQ reviewed the manuscript critically for important intellectual content and appropriate academic content. All authors read and approved the final manuscript.

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Correspondence to Graciela Castro-Escarpulli.

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Escobar-Escamilla, N., Ramírez-González, J.E., Castro-Escarpulli, G. et al. Utility of high-throughput DNA sequencing in the study of the human papillomaviruses. Virus Genes 54, 17–24 (2018). https://doi.org/10.1007/s11262-017-1530-3

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