Abstract
Bipolaris maydis is the causal agent of corn southern leaf blight. Here, we report a novel double-stranded RNA (dsRNA) mycovirus designated Bipolaris maydis botybirnavirus 1 (BmBRV1) from B. maydis strain JZ11 in Jingzhou, Hubei province of China. BmBRV1 has a genome consisting of two dsRNAs (dsRNA1 and dsRNA2) with a size of 6435 and 5987 bp, respectively, each of which contains a single open reading frame (ORF). The two polyproteins encoded by dsRNA1 and dsRNA2 share the highest amino acid identities of 81.8 and 75.3%, respectively, with the RdRp and coat protein of Sclerotinia sclerotiorum botybirnavirus 1 (SsBRV1), a tentative species of the genus Botybirnavirus. Phylogenetic analysis based on the amino acid sequences of RdRp indicated that BmBRV1 belongs to a distinct species of the newly proposed family Botybirnaviridae.
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References
Adams MJ, Lefkowitz EJ, King AMQ, Harrach B, Harrison RL, Knowles NJ, Kropinski AM, Krupovic M, Kuhn JH, Mushegian AR, Nibert M, Sabanadzovic S, Sanfaçon H, Siddell SG, Simmonds P, Varsani A, Zerbini FM, Gorbalenya AE, Davison AJ (2017) Arch Virol 162:2505–2538
Ghabrial SA, Suzuki N (2009) Annu Rev Phytopathol 47:353–384
Ghabrial SA, Caston JR, Jiang DH, Nibert ML, Suzuki N (2015) Virology 479:356–368
Liu LJ, Wang QH, Cheng JS, Fu YP, Jiang DH, Xie JT (2015) Front Microbiol 6:406
Ran HC, Liu LJ, Li B, Cheng JS, Fu YP, Jiang DH, Xie JT (2016) Virol J 13:92
Wu MD, Jin FY, Zhang J, Yang L, Jiang DH, Li GQ (2012) J Virol 86:6605–6619
Xiang J, Fu M, Hong N, Zhai LF, Xiao F, Wang GP (2017) Arch Virol 162:3907–3911
Morris TJ, Dodds JA (1979) Phytopathology 69:854–858
Yeku O, Frohman MA (2011) Methods Mol Biol 703:107
Peng XQ, Pan H, Muhammad A, An HL, Fang SG, Li WM, Zhang SB (2018) Arch Virol 163:805–808
Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) J Mol Biol 215:403–410
Marchler-Bauer A, Derbyshire MK, Gonzales NR, Lu SN, Chitsaz F, Geer LY, Geer RC, He J, Gwadz M, Hurwitz DI, Lanczycki CJ, Lu F, Marchler GH, Song JS, Thanki N, Wang ZX, Yamashita RA, Zhang DC, Zheng CJ, Bryant SH (2015) Nucleic Acids Res 43:D222–D226
Kumar S, Stecher G, Tamura K (2016) Mol Biol Evol 33:1870–1874
Acknowledgements
This research was supported by the National Natural Science Foundation of China (Nos. 31301638, 31501609), and the Opening Fund of Key Laboratory of Biotechnology in Plant Protection of MOA of China and Zhejiang Province. We are grateful to Dr. Qingchao Deng at College of Agriculture, Yangtze University for his help in preparing the manuscript.
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HRW and CL extracted and cloned the dsRNA; LNC identified the fungus; HRW, CL, and SBZ collected the sample; LMZ participated in the cloning experiments; SGF, YL, FY, and SBZ designed the research, and SBZ wrote the paper.
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Wang, H., Li, C., Cai, L. et al. The complete genomic sequence of a novel botybirnavirus isolated from a phytopathogenic Bipolaris maydis. Virus Genes 54, 733–736 (2018). https://doi.org/10.1007/s11262-018-1584-x
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DOI: https://doi.org/10.1007/s11262-018-1584-x