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Anti-herpes simplex virus activity of polysaccharides from Eucheuma gelatinae

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Abstract

Acyclovir is a commonly-used drug for treating herpes simplex virus (HSV) infections, but with its wide clinical application, more and more resistant strains have been found. Therefore, seeking a drug that can act against acyclovir-resistant virus has become an important goal of drug screening and development. In this study, plaque reduction assay, real-time PCR, Western blot, and immunofluorescence technique were used to investigate the antiviral effect of Eucheuma gelatinae polysaccharide (EGP) on HSV and to preliminarily clarify the in vitro anti-HSV mechanism of EGP. EGP was found to significantly inhibit HSV infection in vitro and displayed a good inhibitory effect on acyclovir-resistant strains. More detailed experiments have shown that EGP prevented early HSV-1 infection through directly inactivating HSV-1 particles and impairing virus attachment, but without effect on viral penetration. EGP also inhibited the RNA synthesis of HSV-1 early gene and late gene as well as viral DNA replication; no effect on immediate-early gene synthesis was observed. Besides, through immunofluorescence and western blot, we found that EGP significantly affected the protein synthesis of HSV-1. Taken together, these results demonstrate that EGP exerts its anti-HSV activity mainly through impeding early HSV-1 infection and inhibiting viral RNA and DNA syntheses. The weak cytotoxicity, strong viral inactivation as well as attachment inhibition activity enable EGP to be a virucide candidate for HSV therapy, especially for drug-resistant strains.

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Acknowledgments

This work was supported by the Key Projects in the National Science & Technology Pillar Program during the Twelfth Five-year Plan Period (SQ2011SF12B02099), the National Natural Science Foundation of China (81274170), the Guangdong Marine Economic Development and Innovation of Regional Demonstration Project (GD2012-D01-002), and the PhD Start-up Fund of Natural Science Foundation of Guangdong Province (NO2060203).

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Authors and Affiliations

  1. Biomedicine Research and Development Center, Guangdong Provincial Key Laboratory of Bioengineering Medicine, National Engineering Research Center of Genetic Medicine, Jinan University, Guangzhou, 510632, China

    Fujun Jin, Cuiqin Zhuo, Zhe He, Huailin Wang, Wei Liu & Yifei Wang

  2. Department of Endoscopy, Sun Yat-sen University Cancer Center, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Guangzhou, Guangdong, China

    Rong Zhang

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  1. Fujun Jin
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  2. Cuiqin Zhuo
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  3. Zhe He
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  4. Huailin Wang
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  5. Wei Liu
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  6. Rong Zhang
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  7. Yifei Wang
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Correspondence to Rong Zhang or Yifei Wang.

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Jin, F., Zhuo, C., He, Z. et al. Anti-herpes simplex virus activity of polysaccharides from Eucheuma gelatinae . World J Microbiol Biotechnol 31, 453–460 (2015). https://doi.org/10.1007/s11274-015-1798-1

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  • DOI: https://doi.org/10.1007/s11274-015-1798-1

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