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1H NMR metabolic profiling of human serum associated with benign and malignant gallstone diseases

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Abstract

Proton NMR based metabolic profile of serum associated with different gallbladder pathologies is presented. Quantitative and qualitative variations in the metabolic profile of serum in control samples and three different pathologies of gallbladder, chronic cholecystitis, xanthogranulomatous cholecystitis and carcinoma of gallbladder has been evaluated by use of 1H NMR based metabonomics and multivariate chemometric methods. Multivariate partial least square discriminant analysis of 1H NMR spectra showed a clear discrimination between control and diseased groups on the basis of quantitative and qualitative metabolic variations. Increased levels of lactate and pyruvate whereas decreased levels of glucose, some amino acids and low density lipoprotein/very low density lipoprotein (LDL/VLDL) were observed. These metabolites, responsible for class discrimination, from different metabolic pathways could be considered as the signatures of the carcinoma of gallbladder.

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Abbreviations

CC:

Chronic cholecystitis

XGC:

Xanthogranulomatous cholecystitis

GBC:

Gallbladder Cancer

MALDI–TOF–MS:

Matrix assisted laser desorption/ionization–Time of flight–Mass spectroscopy

PCA:

Principle component analysis

PLS-DA:

Partial least square discriminant analysis

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Acknowledgments

Kanchan Sonkar acknowledges financial support from Council of Scientific and Industrial Research, India. This work was financially support from Department of Biotechnology, India (Grant No. BT/PR12700/BRB/10/719/2009).

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  1. Centre of Biomedical Magnetic Resonance, Sanjay Gandhi Post Graduate Institute of Medical Sciences (SGPGIMS) Campus, Raibarely Road, Lucknow, 226014, Uttar Pradesh, India

    Kanchan Sonkar & Neeraj Sinha

  2. Department of Surgical Gastroenterology, Sanjay Gandhi Post Graduate Institute of Medical Sciences (SGPGIMS), Lucknow, 226014, Uttar Pradesh, India

    Anu Behari & V. K. Kapoor

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  1. Kanchan Sonkar
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Correspondence to Neeraj Sinha.

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Sonkar, K., Behari, A., Kapoor, V.K. et al. 1H NMR metabolic profiling of human serum associated with benign and malignant gallstone diseases. Metabolomics 9, 515–528 (2013). https://doi.org/10.1007/s11306-012-0468-7

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