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A Small Animal Positron Emission Tomography Study of the Effect of Chemotherapy and Hormonal Therapy on the Uptake of 2-Deoxy-2-[F-18]fluoro-d-glucose in Murine Models of Breast Cancer

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Abstract

Purpose

We used small animal positron emission tomography (PET) imaging to monitor the time-course of tumor metabolic response to hormone and chemotherapy in a murine model of hormone-sensitive breast cancer.

Procedures

Estrogen receptor positive murine mammary carcinomas were inoculated in Balb/c mice. Small animal PET imaging using 2-deoxy-2-[F-18]fluoro-d-glucose (FDG) was used to assess tumor metabolic activity. Imaging was done before and at days 1, 7, and 14 after the administration of doxorubicin, methotrexate, letrozole, or placebo. The tumor uptake of FDG was calculated from a region-of-interest drawn around the tumor.

Results

All treatments resulted in a decrease in tumor growth rate and end volume compared to untreated control. FDG uptake was also markedly decreased after treatment although a flare reaction was observed on PET at day 7, the intensity of which varied according to the treatment modality.

Conclusion

PET imaging is sensitive to detect early changes associated with therapy in murine breast cancer models. A flare reaction was observed 7 days after the initiation of therapy.

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Acknowledgments

This project was supported by the Canadian Breast Cancer Research Alliance (CBCRA) grant 015388, the Canadian Institutes of Health Research (CIHR) grant MT-15348 and a “Fonds de la recherche en santé du Québec” salary award program.

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Authors and Affiliations

  1. Sherbrooke Molecular Imaging Center, Department of Nuclear Medicine and Radiobiology, Faculty of Medicine and Health Sciences, Université de Sherbrooke, 3001, 12th Ave N, Sherbrooke, QC, Canada, JIH 5N4

    Antonio Aliaga, Jacques A. Rousseau, Jules Cadorette, Étienne Croteau, Johan E. van Lier, Roger Lecomte & François Bénard

Authors
  1. Antonio Aliaga
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  2. Jacques A. Rousseau
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  3. Jules Cadorette
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  4. Étienne Croteau
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  5. Johan E. van Lier
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  6. Roger Lecomte
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  7. François Bénard
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Corresponding author

Correspondence to François Bénard.

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Aliaga, A., Rousseau, J.A., Cadorette, J. et al. A Small Animal Positron Emission Tomography Study of the Effect of Chemotherapy and Hormonal Therapy on the Uptake of 2-Deoxy-2-[F-18]fluoro-d-glucose in Murine Models of Breast Cancer. Mol Imaging Biol 9, 144–150 (2007). https://doi.org/10.1007/s11307-007-0091-6

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  • DOI: https://doi.org/10.1007/s11307-007-0091-6

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