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Oxidative and carbonyl stress in patients with obstructive sleep apnea treated with continuous positive airway pressure

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Abstract

Purpose

The pathogenesis of cardiovascular complications of obstructive sleep apnea syndrome (OSAS) can be explained by oxidative and carbonyl stress due to oxygenation and reoxygenation injury during sleep. This hypothesis has yet to be proved experimentally, although several clinical observations have found increased oxidative damage in plasma. Continuous positive airway pressure (CPAP) improves symptoms and prognosis of patients with OSAS.

Methods

Patients with confirmed SAS (n = 89) underwent polysomnography and received CPAP treatment. Plasma and saliva samples were taken before CPAP therapy as well as after 1 and 6 months of CPAP treatment. Selected markers of oxidative and carbonyl stress were measured in plasma and saliva, and their dynamics was statistically analyzed.

Results

Plasma levels of thiobarbituric acid reacting substances—a marker of lipoperoxidation—and advanced glycation end products (AGEs)—a marker of carbonyl stress—were decreased by the CPAP therapy. The decrease of AGEs and fructosamine was also found in saliva. Interestingly, no gender differences and no changes of antioxidant status measured as total antioxidant capacity and ferrous reducing ability were found in either of the samples.

Conclusion

Previous findings of lowered plasma markers of oxidative stress were confirmed. Plasma AGEs were lowered by CPAP therapy. This is the first study analyzing markers of oxidative and carbonyl stress in saliva. Non-invasive sampling of saliva makes it a very interesting source of information for repeated monitoring of therapy success. Salivary AGEs and fructosamine as markers of carbonyl stress were decreased by the CPAP therapy and might therefore have potential informative value for clinical observations, as well as for the understanding of the pathogenesis of OSAS complications.

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Acknowledgments

Peter Celec designed the study, performed biochemical analyses, and wrote the manuscript. Michal Behuliak, Roland Pálffy, and Roman Gardlík performed the biochemical analyses. Lukáč Halčák and Július Hodosy managed the study and the analyses. Imrich Mucska contacted the patients and collected samples.

The authors would like to thank all the patients for participating in the study. This study was sponsored by the Slovak Research and Development Agency, grant VMSP-II-0027-09.

Conflict of interest for all authors

None to declare

Author information

Authors and Affiliations

  1. Institute of Molecular Biomedicine, Comenius University, Sasinkova 4, 811 08, Bratislava, Slovakia

    Peter Celec, Július Hodosy, Michal Behuliak & Roland Pálffy

  2. Institute of Pathophysiology, Comenius University, Bratislava, Slovakia

    Peter Celec, Michal Behuliak & Roland Pálffy

  3. Department of Molecular Biology, Comenius University, Bratislava, Slovakia

    Peter Celec

  4. Institute of Physiology, Comenius University, Bratislava, Slovakia

    Július Hodosy

  5. Institute of Molecular Biomedicine, Comenius University, Bratislava, Slovakia

    Roman Gardlík

  6. Institute of Chemistry, Biochemistry and Clinical Biochemistry, Comenius University, Bratislava, Slovakia

    Lukáč Halčák

  7. Sleep Laboratory, University Hospital, Comenius University, Bratislava, Slovakia

    Imrich Mucska

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  1. Peter Celec
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  2. Július Hodosy
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  3. Michal Behuliak
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  4. Roland Pálffy
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  6. Lukáč Halčák
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  7. Imrich Mucska
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Correspondence to Peter Celec.

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Celec, P., Hodosy, J., Behuliak, M. et al. Oxidative and carbonyl stress in patients with obstructive sleep apnea treated with continuous positive airway pressure. Sleep Breath 16, 393–398 (2012). https://doi.org/10.1007/s11325-011-0510-4

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  • DOI: https://doi.org/10.1007/s11325-011-0510-4

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