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Adiponectin protects the kidney against chronic intermittent hypoxia-induced injury through inhibiting endoplasmic reticulum stress

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Abstract

Purpose

The current study was carried out to assess the effects of chronic intermittent hypoxia (CIH) on the kidney, the intervention roles of adiponectin (Ad), and the associated mechanisms.

Methods

Sixty Wistar rats were randomly divided into four groups: the normal control (NC), normal control plus Ad supplement (NC + Ad), CIH, and CIH plus Ad supplement (CIH + Ad) groups. The rats in both CIH and CIH + Ad groups were submitted to a CIH environment for 4 months, while the rats in NC and NC + Ad groups were housed with the normal air for 4 months. In addition, the rats in NC + Ad and CIH + Ad groups were treated with an intravenous injection of Ad at a dosage of 10 μg per injection, twice a week, for four successive months.

Results

The production level of reactive oxygen species (ROS) and the protein levels of endoplasmic reticulum (ER) stress, as well as the cell apoptosis level in kidney, were all higher in the CIH group than in the NC and NC + Ad groups (all p < 0.05). However, the ROS production, the protein of ER stress, and cell apoptosis levels in kidney were all lower in the CIH + Ad group than those in the CIH group (all p < 0.05).

Conclusion

Ad could protect against CIH-induced renal cell apoptosis through inhibiting ROS-related ER stress.

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Acknowledgments

We are grateful to PIs of the department of cardiology lab.

Author information

Authors and Affiliations

  1. Department of Respiratory, The First Affiliated Hospital with Nanjing Medical University, 300 Guangzhou Road, Nanjing, 210029, China

    Wenxiao Ding, Yuanpei Cai, Wenjing Wang, Lingling Ji, Yanbin Dong, Xiaofeng Zhang, Mei Su & Xilong Zhang

  2. Department of Respiratory Diseases, Jiangsu Geriatric Hospital, 42 Jiangsu Road, Nanjing, 210029, China

    Jiannan Liu & Gan Lu

Authors
  1. Wenxiao Ding
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  2. Yuanpei Cai
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  3. Wenjing Wang
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  4. Lingling Ji
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  5. Yanbin Dong
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  6. Xiaofeng Zhang
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  7. Mei Su
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  8. Jiannan Liu
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  9. Gan Lu
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  10. Xilong Zhang
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Corresponding authors

Correspondence to Gan Lu or Xilong Zhang.

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Conflict of interest

The authors declare that they have no conflict of interest.

Funding

The study was supported by the National Natural Science Foundation of China (81370184 and 81500071). The sponsor had no role in the design or conduct of this research.

Ethical approval

All applicable international, national, and institutional guidelines for the care and use of animals were followed. The Animal Ethics Committee of Nanjing Medical University approved this study.

Additional information

Wenxiao Ding contributed equally to Yuanpei Cai

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Ding, W., Cai, Y., Wang, W. et al. Adiponectin protects the kidney against chronic intermittent hypoxia-induced injury through inhibiting endoplasmic reticulum stress. Sleep Breath 20, 1069–1074 (2016). https://doi.org/10.1007/s11325-016-1321-4

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  • DOI: https://doi.org/10.1007/s11325-016-1321-4

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