Abstract
Recently, n-3 fatty acids are in the center of attention for their potent anti-inflammatory effects. Osteoporosis as a chronic senile disease is associated with inflammation, and the role of inflammatory mediators has been demonstrated in recent years. The beneficial effects of n-3 fatty acids on bone were proven in many animal studies, while to date, no conclusive data is available in human. The aim of this study was to evaluate the impact of n-3 fatty acids on bone biomarkers in osteoporotic postmenopausal women. Twenty-five osteoporotic postmenopausal women were recruited in the study and randomized in treatment and control groups. The patients received 900 mg n-3 fatty acid capsules or placebo per day for 6 months. Serum levels of osteocalcin, bone alkaline phosphatase (BALP), calcium, vitamin D, and parathormone and urine concentration of pyridinoline (Pyd) were measured at baseline, second month, and sixth month in both groups. In the treatment group, compared with baseline, at the second month, osteocalcin increased slightly; thereafter, it showed decrement trend until the end of the study. In the control group, it decreased all over the study. None of these changes was significant. BALP showed nonsignificant decrease from baseline over the time in both groups. Urine level of Pyd decreased significantly (P < 0.05) in the treatment group, while no significant change was seen in the control group. Serum calcium and vitamin D increased in both groups; however, changes were not significant. No significant changes were seen in calcium clearance and parathormone. In conclusion, n-3 fatty acids can decrease bone resorption; however, it could not affect bone formation significantly after 6 months treatment. Further investigations are recommended.
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References
Abdollahi M, Larijani B, Rahimi R, Salari P (2005) Role of oxidative stress in osteoporosis. Therapy 2(5):787–796
Anonymous (2001) Osteoporosis prevention, diagnosis, and therapy. JAMA 285:785–795
Baggio B, Budakovic A, Ferraro A, Checchetto S, Priante G, Musacchio E, Manzato E, Zaninotto M, Maresca MC (2005) Relationship between plasma phospholipid polyunsaturated fatty acid composition and bone disease in renal transplantation. Transplantation 80:1349–1352
Bassey EJ, Littlewood JJ, Rothwell MC, Pye DW (2000) Lack of effect of supplementation with essential fatty acids on bone mineral density in health pre- and postmenopausal women: two randomized controlled trials of Efacal® v. calcium alone. Br J Nutr 83:629–635
Chrischilles E, Shireman T, Wallace R (1994) Costs and health effects of osteoporotic fractures. Bone 15(4):377–386
Cremers S, Garnero P (2006) Biochemical markers of bone turnover in the clinical development of drugs for osteoporosis and metastatic bone disease. Drugs 66(16):2031–2058
Eriksen EF, Gundersen HJG, Melsen F, Mosekilde L (1984) Reconstruction of the formative site in iliac trabecular bone in 20 normal individuals employing a kinetic model for matrix and mineral apposition. Metab Bone Dis Relat Res 5:243–252
Gilman J, Cashman KD (2007) The effect of marine oil-derived n-3 fatty acids on transepithelial calcium transport in Caco-2 cell models of healthy and inflamed intestines. Br J Nutr 97:281–288
Griel AE, Kris-Etherton PM, Hilpert KF, Zhao G, West SG, Corwin RL (2007) An increase in dietary n-3 fatty acids decreases a marker of bone resorption in humans. Nutr J 6:2
Hőgstrom M, Nordstrőm P, Nordstrőm A (2007) n-3 fatty acids are positively associated with peak bone mineral density and bone accrual in healthy men: the NO2 study. Am J Clin Nutr 85:803–807
Kruger MC, Coetzer H, de Winter R et al (1998) Calcium, gammalinolenic acid and eicosapentaenoic acid supplementation in senile osteoporosis. Aging Clin Exp Res 10(5):385–394
Larijani B, Moayyeri A, Keshtkar AA et al (2006) Peak bone mass of Iranian population: the Iranian Multicenter Osteoporosis Study. J Clin Densitom 9(3):367–374
Lucey AJ, Paschos GK, Cashman KD, Martίnéz JA, Thorsdottir I, Kiely M (2008) Influence of moderate energy restriction and seafood consumption on bone turnover in overweight young adults. Am J Clin Nutr 87:1045–1052
Macdonald HM, New SA, Golden MH et al (2004) Nutritional associations with bone loss during the menopausal transition: evidence of a beneficial effect of calcium, alcohol, and fruit and vegetable nutrients and of a detrimental effect of fatty acids. Am J Clin Nutr 79(1):155–165
Martίnez-Ramίrez MJ, Palma S, Martίnez-González MA, Delgado-Martίnez AD, de la Fuente C, Delgado-Rodrίguez M (2007) Dietary fat intake and the risk of osteoporotic fractures in the elderly. Eur J Nutr 61(9):1114–1120
Salari P, Larijani B, Abdollahi M (2008a) Association of hyperhomocysteinemia with osteoporosis: a systematic review. Therapy 5(2):215–222
Salari P, Rezaie A, Larijani B, Abdollahi M (2008b) A systematic review of the impact of n-3 fatty acids in bone health and osteoporosis. Med Sci Monit 14(3):RA37–RA44
Shen CL, Yeh JK, Rasty J et al (2006) Protective effect of dietary long-chain n-3 polyunsaturated fatty acids on bone loss in gonad-intact middle-aged male rats. Br J Nutr 95:462–468
Silverstrini G, Ballanti P, Patacchioli F et al (2005) Detection of osteoprotegerin (OPG) and its ligand (RANKL) mRNA and protein in femur and tibia of rat. J Mol Histol 36(1–2):59–67
Sun L, Tamaki H, Ishimaru T et al (2004) Inhibition of osteoporosis due to restricted food intake by the fish oils DHA and EPA and Perilla oil in the rat. Biosci Biotechnol Biochem 68:2613–2615
Twiss JJ, Waltman NL, Berg K, Ott CD, Gross GJ, Lindsey AM (2009) An exercise intervention for breast cancer survivors with bone loss. J Nurs Scholarsh 41(1):20–27
van Papendrop DH, Coetzer H, Kruger MC (1995) Biochemical profile of osteoporotic patients on essential faty acid supplementation. Nutr Res 15(3):325–334
Watkins BA, Li Y, Seifert MF (2006) Dietary ration of n-6/n-3 PUFAs and DHA: actions on bone mineral and serum biomarkers in ovariectomized rats. J Nutr Biochem 17(4):282–289
Weiss LA, Barrett-Connor E, von Műhlen D (2005) Ratio of n-6 to n-3 fatty acids and bone mineral density in older adults: the Rancho Bernardo Study. Am J Clin Nutr 81:934–938
Yasuda H, Shima N, Nakagawa N et al (1998) Osteoclast differentiation factor is a ligand for osteoprotegerin/osteoclastogenesis-inhibitory factor and is identical to TRANCE/RANKL. Proc Natl Acad Sci U S A 95(7):3597–3602
Yousefzadeh G, Larijani B, Mohammadirad A, Heshmat R, Dehghan G, Rahimi R, Abdollahi M (2006) Determination of oxidative stress status and concentration of TGF-beta 1 in the blood and saliva of osteoporotic subjects. Ann N Y Acad Sci 1091:142–150
Acknowledgment
This study was supported by a grant from Endocrinology and Metabolism Research Center, Tehran University of Medical Sciences. Zahravi Pharmaceutical Company provided the drug and placebo. Authors wish to thank the physicians and nurses who helped in sampling and technicians who assisted in measurements.
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Salari Sharif, P., Asalforoush, M., Ameri, F. et al. The effect of n-3 fatty acids on bone biomarkers in Iranian postmenopausal osteoporotic women: a randomized clinical trial. AGE 32, 179–186 (2010). https://doi.org/10.1007/s11357-009-9122-3
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DOI: https://doi.org/10.1007/s11357-009-9122-3