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Development of a serum profile for healthy aging

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Abstract

Increasing numbers of Americans are reaching 85 years of age or older, yet there are no reliable biomarkers to predict who will live this long. The goal of this pilot study therefore was: (1) to identify a potential serum pattern that could identify proteins involved in longevity and (2) to determine if this pattern was a marker of longevity in an independent sample of individuals. Serum samples were analyzed in three cohorts of individuals (n = 12 in each) aged 20–34, 60–74, and ≥90 years who participated in The Louisiana Healthy Aging Study. The 12 most abundant proteins were removed and the remaining proteins separated by two-dimensional gel electrophoresis. Gels were matched and the intensity of each spot quantified. Multivariate discriminant analysis was used to identify a serum pattern that could separate these three age cohorts. Seven protein spots were found that correctly distinguished the subjects into the three groups. However, these spots were not as successful in discriminating the ages in a second set of 15 individuals as only eight of these subjects were placed into their correct group. These preliminary results show that the proteomics approach can be used to identify potential proteins or markers that may be involved in the aging process and/or be important determinants of longevity.

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Acknowledgments

This work was supported by the Louisiana Board of Regents through the Millennium Trust Health Excellence Fund (HEF (2001-06) 02) and by the National Institute on Aging (PO1 AGO22064).

We would like to acknowledge the efforts of Dr. Doug Hinerfeld at the Proteomics Core at the University of Massachusetts Medical School who prepared the 2-D gels.

We acknowledge and thank everyone working on the Louisiana Healthy Aging Study from Pennington Biomedical Research Center, Louisiana State University, Baton Rouge; Louisiana State University Health Sciences Center, Tulane University, New Orleans; and University of Alabama at Birmingham: S. Michal Jazwinski, PhD, Mark Batzer, PhD, Pauline Callinan, Jerilyn Walker, Scott W. Herke, DeQuindra Rouzan, Jennifer Arceneaux, RN, Andrew Pellett, PhD, Henry Rothschild, MD, PhD, Crystal Traylor, APRN, MSN, WHNP, David Welsh, MD, Joseph Su, PhD, Yu-wen Chiu, Elizabeth Fontham, PhD, Cruz Velasco-Gonzalez, PhD, Jennifer Hayden, Matthew Leblanc, Li Li, MD, Sangkyu Kim, Hui-Yi Lin, PhD, Beth Schmidt, Jessi Thomspon, PhD, Valentina Greco, PhD, Beth Kimball, Meghan Allen, Donald Scott, PhD, John Mountz, PhD, MD, Hui-Chen Hsu, PhD, Pili Zhang, Kim Pederson, Juling Zhou, PhD, Tiffany Hall, Kim Landry, Mandy Shipp, Anita Smith, Lauri Byerley, PhD, James P DeLany, PhD, Robert Schwartz, PhD, Evest Broussard, Michael Businelle, Paula Geiselman, PhD, Darla Kendzor, Vijay Hegde, PhD, Robert Wood, PhD, Michael Welsch, PhD, Iina E. Antikainen, Fernanada Holton, Carl Lavie, MD, Artie Brown, Ryan Russell, Devon Dobrosielski, Arturo Ace, Katie Cherry, PhD, Karri Hawley, PhD, Emily Olinde, Jenny Denver, and Kay Lopez, DSN.

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Authors and Affiliations

  1. Physiology Department, Louisiana State University Health Science Center, 1901 Perdido St, New Orleans, LA, 70112, USA

    Lauri O. Byerley & Chau-Wen Chou

  2. Biology Department, University of North Carolina at Charlotte, 9201 University City Blvd, Charlotte, NC, 28223, USA

    Lauri O. Byerley & Larry Leamy

  3. Pennington Biomedical Research Center, 6400 Perkins Rd, Baton Rouge, LA, 70808, USA

    Lauri O. Byerley & Eric Ravussin

  4. University of Massachusetts Medical School, Worcester Foundation Campus, Fuller Building, 222 Maple Avenue, Shrewsbury, MA, 01545, USA

    Sun W. Tam

Authors
  1. Lauri O. Byerley
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  2. Larry Leamy
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  3. Sun W. Tam
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  4. Chau-Wen Chou
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  5. Eric Ravussin
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For the Louisiana Healthy Aging Study

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Correspondence to Lauri O. Byerley.

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Byerley, L.O., Leamy, L., Tam, S.W. et al. Development of a serum profile for healthy aging. AGE 32, 497–507 (2010). https://doi.org/10.1007/s11357-010-9146-8

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  • DOI: https://doi.org/10.1007/s11357-010-9146-8

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