Skip to main content
SpringerLink
Log in

Quantitative determination of atractylon in Atractylodis Rhizoma and Atractylodis Lanceae Rhizoma by 1H-NMR spectroscopy

  • Original Paper
  • Published:
Journal of Natural Medicines Aims and scope Submit manuscript

Abstract

1H-NMR spectroscopy was successfully applied to the quantitative determination of atractylon in Atractylodis Rhizoma (dried rhizomes of Atractylodes ovata and A. japonica) and Atractylodis Lanceae Rhizoma (dried rhizomes of Atractylodes lancea and A. chinensis). The analysis was carried out by comparing the integral of the H-12 singlet signal of atractylon, which was well separated in the range of δ 6.95–7.05 ppm in the NMR spectrum, with the integral of a hexamethyldisilane (HMD) signal at δ 0 ppm. The atractylon contents obtained by the 1H-NMR spectroscopy were consistent with those obtained by the conventional HPLC analysis. The present method requires neither reference compounds for calibration curves nor sample pre-purification. It also allows simultaneous determination of multiple constituents in a crude extract. Thus, it is applicable to chemical evaluation of crude drugs as a powerful alternative to various chromatographic methods.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  1. Prieto JM, Recio MC, Giner RM, Máñez S, Giner-Larza EM, Ríos JL (2003) Influence of traditional Chinese anti-inflammatory medicinal plants on leukocyte and platelet functions. J Pharm Pharmacol 55:1275–1282

    Article  CAS  PubMed  Google Scholar 

  2. Wang KT, Chen LG, Yang LL, Ke WM, Chang HC, Wang CC (2007) Analysis of the sesquiterpenoids in processed Atractylodis Rhizoma. Chem Pharm Bull 55:50–56

    Article  CAS  PubMed  Google Scholar 

  3. Wang CC, Chen LG, Yang LL (2002) Cytotoxic activity of sesquiterpenoids from Atractylodes ovata on leukemia cell lines. Planta Med 68:204–208

    Article  CAS  PubMed  Google Scholar 

  4. Kim HK, Yun YK, Ahn YJ (2007) Toxicity of atractylon and atractylenolide III identified in Atractylodes ovata rhizome to Dermatophagoides farinae and Dermatophagoides pteronyssinus. J Agric Food Chem 55:6027–6031

    Article  CAS  PubMed  Google Scholar 

  5. Satoh K, Nagai F, Ushiyama K, Kano I (1996) Specific inhibition of Na+,K+-ATPase activity by atractylon, a major component of Byaku-jutsu, by interaction with enzyme in the E2 state. Biochem Pharmacol 51:339–343

    Article  CAS  PubMed  Google Scholar 

  6. Ministry of Health and Welfare (2000) The Japanese Pharmacopeia, 15th edn, Japan, pp 11–12; see also pp 13–14

  7. Alberti F, Belton PS, Gil AM (2002) Application of NMR to food science. Annu Rep NMR Spectrosc 47:109–148

    Article  CAS  Google Scholar 

  8. Tahara M, Sugimoto N, Suematsu T, Arifuku K, Saito T, Ihara T, Yoshida Y, Tada A, Kubota R, Shimizu K, Yamazaki T, Tanamoto K, Nakazawa H, Nishimura T (2009) Quality control of organophophorous isoxanthion oxon based on qNMR. Jpn J Food Chem Safety 16:28–33

    CAS  Google Scholar 

  9. Pauli GF, Jaki BU, Lankin GC (2005) Quantitative 1H NMR: development and potential of a method for natural products analysis. J Nat Prod 68:133–149

    Article  CAS  PubMed  Google Scholar 

  10. Guo Y, Kondo K, Terabayashi S, Yamamoto Y, Shimada H, Fujita M, Kawasaki T, Maruyama T, Goda Y, Mizukami H (2006) DNA authentication of So-jutsu (Atractylodes lancea rhizome) and Byaku-jutsu (Atractylodes rhizome) obtained in the market based on the nucleotide sequence of the 18S–5.8S rDNA internal transcribed spacer region. J Nat Med 60:149–156

    Article  CAS  Google Scholar 

  11. Zhao C, He C (2006) Preparative isolation and purification of atractylon and atractylenolide III from the Chinese medicinal plant Atractylodes macrocephala by high-speed counter-current chromatography. J Sep Sci 29:1630–1636

    Article  CAS  PubMed  Google Scholar 

  12. Saito T, Ihara T, Koike M, Kinugasa S, Fujimine Y, Nose K, Hira T (2009) A new traceability scheme for the development of international system-traceable persistent organic pollutant reference materials by quantitative nuclear magnetic resonance. Accred Qual Assur 14:79–86

    Article  CAS  Google Scholar 

  13. Takeda O, Miki E, Morita M, Okada M, Lu Y, He SA (1994) Variation of essential oil components of Atractylodes lancea growing in Mt. Maoshan area in Jiangsu Province, China. Nat Med 48:11–17

    CAS  Google Scholar 

  14. Kohda H, Goto K, Anetai M, Yamagishi T (1994) Studies on the botanical origin of Atractylodes lancea “Cang Zhu”. Nat Med 48:58–62

    Google Scholar 

  15. Mizukami H, Shimizu R, Kohda H, Kohjyouma M, Kawanishi F, Hiraoka N (1996) Restriction fragment length polymorphisms of rDNA and variation of essential oil composition in Atractylodes plants. Biol Pharm Bull 19:577–580

    CAS  PubMed  Google Scholar 

Download references

Acknowledgments

We would like to thank Dr. Y. Yamamoto, Tochimoto tenkaido Co., Ltd., and Dr. M Fujita, Uchida Wakanyaku Co., Ltd., for providing us with crude drug samples of Atractylodis Rhizoma and Atractylodis Lanceae Rhizoma. This work is supported by Grant-in-Aid for Scientific Research from the Ministry of Health, Welfare and Labor of Japan.

Author information

Authors and Affiliations

  1. Graduate School of Pharmaceutical Sciences, Nagoya City University, 3-1 Tanabe-dori, Mizuho-ku, Nagoya, 467-8603, Japan

    Keiko Hasada, Takamitsu Yoshida & Hajime Mizukami

  2. College of Pharmacy, Kinjo Gakuin University, 2-1723 Omori, Moriyama-ku, Nagoya, 463-8521, Japan

    Keiko Hasada & Akito Nagatsu

  3. National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo, 158-8501, Japan

    Takeshi Yamazaki, Naoki Sugimoto & Tetsuji Nishimura

Authors
  1. Keiko Hasada
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Takamitsu Yoshida
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Takeshi Yamazaki
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Naoki Sugimoto
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Tetsuji Nishimura
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Akito Nagatsu
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Hajime Mizukami
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Hajime Mizukami.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hasada, K., Yoshida, T., Yamazaki, T. et al. Quantitative determination of atractylon in Atractylodis Rhizoma and Atractylodis Lanceae Rhizoma by 1H-NMR spectroscopy. J Nat Med 64, 161–166 (2010). https://doi.org/10.1007/s11418-010-0393-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11418-010-0393-x

Keywords

Search

Navigation