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Replication-incompetent adenovirus vector-mediated MDA-7/IL-24 selectively induces growth suppression and apoptosis of hepatoma cell Line SMMC-7721

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Summary

In order to investigate the effect of replication-incompetent adenovirus vector expressing MDA-7/IL-24 on tumor growth and apoptosis of human hepatocellular carcinoma (HCC) cell line SMMC-7721 and normal liver cell line L02, the recombinant replication-incompetent Ad.mda-7 virus vector was constructed and infected into the HCC cell line SMMC-7721 and normal liver cell line L02. RT-PCR was performed to examine the expression of MDA-7 mRNA. The concentrations of MDA-7/IL-4 in culture supernatants were determined by using ELISA. MTT and Hoechst staining assay were applied to observe the inhibitory and killing effects of MDA-7 on the HCC cells. By using flow cytometry, the apoptosis, cell cycle and proliferation of SMMC-7721 and L02 cells were measured. The results showed recombinant replication-incompetent virus expressing MDA-7/IL-24 was constructed successfully, and RT-PCR revealed that it could mediate the high expression of the exogenous gene MDA-7/IL-24 in SMMC-7721 and L02 cells. The expression of MDA-7/IL-24 proteins in the culture supernatant was detectable by ELISA. Ad.mda-7 infection induced apoptosis and growth suppression in SMMC-7721 cells and an increased percentage of HCC cells in the G2/M phase of the cell cycle, but not in L02 cells. It was concluded that mda-7/IL-24 gene, mediated with replication-incompetent adenovirus vector, could selectively induce growth suppression and apoptosis in HCC cell line SMMC-7721 but without any toxic side-effect on normal liver line L02.

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Authors and Affiliations

  1. Department of Biliary and Pancreatic Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China

    Congjun Wang  (王从俊), Xinbo Xue  (薛新波), Jilin Yi  (易继林), Zaide Wu  (吴在德), Kun Chen  (陈 堃), Jianwei Zheng  (郑建伟), Wenwei Ji  (吉文伟) & Yuan Yu  (于 愿)

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  1. Congjun Wang  (王从俊)
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  2. Xinbo Xue  (薛新波)
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  3. Jilin Yi  (易继林)
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  4. Zaide Wu  (吴在德)
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  5. Kun Chen  (陈 堃)
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  6. Jianwei Zheng  (郑建伟)
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  7. Wenwei Ji  (吉文伟)
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  8. Yuan Yu  (于 愿)
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Corresponding author

Correspondence to Xinbo Xue  (薛新波).

Additional information

Congjun WANG, male, born in 1971, M.D., Ph.D.

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Wang, C., Xue, X., Yi, J. et al. Replication-incompetent adenovirus vector-mediated MDA-7/IL-24 selectively induces growth suppression and apoptosis of hepatoma cell Line SMMC-7721. J. Huazhong Univ. Sci. Technol. [Med. Sci.] 28, 80–83 (2008). https://doi.org/10.1007/s11596-008-0120-y

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  • DOI: https://doi.org/10.1007/s11596-008-0120-y

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