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Downregulation of LncRNAH19 and MiR-675 promotes migration and invasion of human hepatocellular carcinoma cells through AKT/GSK-3β/Cdc25A signaling pathway

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Summary

LncRNAH19 has been implicated as having both oncogenic and tumor suppression properties in cancer. LncRNAH19 transcripts also serve as a precursor for miR-675. However, it is unknown whether LncRNAH19 and miR-675 are involved in the migration and invasion of hepatocellular carcinoma (HCC) cells. The purpose of this study was to investigate the effect and mechanism of LncRNAH19 and miR-675 on migration and invasion of HCC cells. The migration and invasion of HCC cells were measured by Transwell migration and invasion assays after transfection of HCC cells with miR-675 inhibitors and LncRNAH19siRNA. The levels of LncRNAH19 and miR-675 were detected by quantitative reverse transcriptase real-time polymerase chain reaction (qRT-PCR), and the protein expression of AKT, GSK-3β and Cdc25A by Western blotting analysis. The expression levels of LncRNAH19 and miR-675 were higher in MHCC-97H cells than in L02, Huh-7 and HepG2 cells. Transwell migration assay revealed that the miR-675 inhibitor and LncRNAH19siRNA could significantly increase the migration of HCC cells (P<0.01) as compared with the control group. Transwell invasion assay demonstrated that the miR-675 inhibitor and LncRNAH19siRNA could significantly increase the invasion of HCC cells (P<0.01) as compared with the control group. Western blotting analysis showed that the expression levels of AKT and Cdc25A were significantly increased (P<0.05), and the expression level of GSK-3β was significantly decreased (P<0.05) after treatment with miR-675 inhibitors and LncRNAH19siRNA as compared with the control group. These findings suggested that inhibition of LncRNAH19 and miR-675 expression can promote migration and invasion of HCC cells via AKT/GSK-3β/Cdc25A signaling pathway.

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Authors and Affiliations

  1. Department of Hepatobiliary Surgery, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou, 510080, China

    Jun Lv  (吕 军), Ling Ma  (马 宁), Xi-lin Chen  (陈锡林) & Qian Wang  (汪 谦)

  2. Department of Surgical Laboratory, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou, 510080, China

    Xiao-hui Huang  (黄晓卉)

  3. Department of Hepatobiliary Surgery, the Affiliated Hospital of Guilin Medical College, Guilin, 541001, China

    Jun Lv  (吕 军)

Authors
  1. Jun Lv  (吕 军)
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  2. Ling Ma  (马 宁)
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  3. Xi-lin Chen  (陈锡林)
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  4. Xiao-hui Huang  (黄晓卉)
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  5. Qian Wang  (汪 谦)
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Corresponding authors

Correspondence to Xiao-hui Huang  (黄晓卉) or Qian Wang  (汪 谦).

Additional information

This project was supported by grants from the National Natural Science Foundation of China (Nos. 81071871, 81101862 and 81172079), Natural Science Foundation of Guangdong Province, China (Nos. S2013010016831, and 10451008901006014), Science and Technology Planning Project of Guangdong Province, China (No. 2009B030801014, 2010B060500007 and 2011B060300012), the Foundation of the Health Department of Guangxi Province, China (No. Z2007212), the Foundation of Scientific Research and Technology Development Project of Guilin, China (key scientific and technological projects and trial production of new products, No. 20110321), the Foundation of Scientific Research and Technology Development Project of Guangxi Province, China (No. GuiKeGong1355005-3-5), and Foundation for Youth Teacher by Sun Yat-Sen University (No. 11ykpy16).

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Lv, J., Ma, L., Chen, Xl. et al. Downregulation of LncRNAH19 and MiR-675 promotes migration and invasion of human hepatocellular carcinoma cells through AKT/GSK-3β/Cdc25A signaling pathway. J. Huazhong Univ. Sci. Technol. [Med. Sci.] 34, 363–369 (2014). https://doi.org/10.1007/s11596-014-1284-2

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  • DOI: https://doi.org/10.1007/s11596-014-1284-2

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