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Dectin-1 rs3901533 and rs7309123 Polymorphisms Increase Susceptibility to Pulmonary Invasive Fungal Disease in Patients with Acute Myeloid Leukemia from a Chinese Han Population

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Summary

This study aimed to assess whether genetic variants of dendritic cell-associated C-type lectine-1 (Dectin-1), Toll-like receptor 2 (TLR2), Toll-like receptor 4 (TLR4), and myeloid differentiation primary response 88 (MyD88) influence the susceptibility to pulmonary invasive fungal disease (IFD) in patients with acute myeloid leukemia (AML) from a Chinese Han population. Eight single nucleotide polymorphisms (SNPs) of Dectin-1 (rs16910526, rs3901533, and rs7309123), TLR2 (rs5743708), TLR4 (rs4986790 and rs4986791) and MyD88 (rs4988453 and rs4988457) in the genomic DNA of 172 adult AML patients were genotyped. Pulmonary IFD was diagnosed as proven or probable according to the 2008 European Organization for Research and Treatment of Cancer/Invasive Fungal Infections Cooperative Group and the National Institute of Allergy and Infectious Diseases Mycoses Study Group (EORTC/MSG) consensus guidelines. SNPs that were significant in the univariate analysis were further analyzed using the multiple logistic regression analysis to determine their association with the occurrence of pulmonary IFD. The mRNA expression of Dectin-1 was detected according to the genotype by quantitative realtime PCR (qRT-PCR), and the correlation of this expression with the occurrence of pulmonary IFD in AML patients was analyzed. Two Dectin-1 intron SNPs (rs3901533 and rs7309123) were found to be significantly associated with the susceptibility to pulmonary IFD in AML patients in a Chinese Han population. Significant associations were noted between pulmonary IFD and Dectin-1 rs3901533 dominant model (G/T+G/G vs. T/T, OR: 2.158; 95% CI: 1.109–4.2, P=0.02), Dectin-1 rs3901533 G allele (OR: 2.201; 95% CI: 1.206–4.019, P=0.01), or Dectin-1 rs7309123 C allele (OR: 1.919; 95% CI: 1.047–3.518, P=0.03). There were no significant associations between pulmonary IFD and the remaining Dectin-1 SNPs (rs16910526), TLR2 (rs5743708), TLR4 (rs4986790 and rs4986791) or MyD88 (rs4988453 and rs4988457). In conclusion, two Dectin-1 SNPs (rs3901533 and rs7309123) are associated with increased susceptibility to pulmonary IFD in AML patients in a Chinese Han population.

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Author notes

  1. Both authors contributed equally to this work.

Authors and Affiliations

  1. The School of Nursing, Fujian Medical University, Fuzhou, 350122, China

    Mei-jing Chen, Rong Hu, Xiao-ying Jiang, Jing-yi Chen & Li Zhan

  2. Institute of Hematology, Department of Hematology, Union Hospital, Fujian Medical University, Fuzhou, 350001, China

    Yong Wu & Zhi-peng He

Authors
  1. Mei-jing Chen
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  2. Rong Hu
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  3. Xiao-ying Jiang
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  4. Yong Wu
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  5. Zhi-peng He
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  6. Jing-yi Chen
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  7. Li Zhan
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Correspondence to Xiao-ying Jiang or Yong Wu.

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Conflict of Interest Statement

The authors declare no conflict of interests.

This study was supported by a grant from the National Natural Science Foundation of China (No. 81500105).

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Chen, Mj., Hu, R., Jiang, Xy. et al. Dectin-1 rs3901533 and rs7309123 Polymorphisms Increase Susceptibility to Pulmonary Invasive Fungal Disease in Patients with Acute Myeloid Leukemia from a Chinese Han Population. CURR MED SCI 39, 906–912 (2019). https://doi.org/10.1007/s11596-019-2122-3

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  • DOI: https://doi.org/10.1007/s11596-019-2122-3

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