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ECV304 (endothelial) is really T24 (bladder carcinoma): Cell line cross-contamination at source

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References

  • Bubenik, J.; Baresova, M.; Viklicky, V.; Jakoubkova, J.; Sainerova, H.; Donner, J. Established cell line of urinary bladder carcinoma (T24) containing tumour-specific antigen. Int. J. Cancer 11:765–773; 1973.

    Article  PubMed  CAS  Google Scholar 

  • Drexler, H. G.; Dirks, W.; MacLeod, R. A. F.; Quentmeier, H.; Steube, K. S.; Uphoff, C. C., ed. DSMZ catalogue of human and animal cell cultures. 7th Ed. Braunschweig, Germany, 1999.

  • Drexler, H. G.; Matsuo, Y. Guidelines for the characterization and publication of human malignant hematopoietic cell lines. Leukemia (in press).

  • Fleckenstein, E.; Uphoff, C. C.; Drexler, H. G. Effective treatment of mycoplasma contamination in cell lines with enrofloxazin (Baytril). Leukemia 8:1424–1434; 1994.

    PubMed  CAS  Google Scholar 

  • Fuentes, E.; Nadal, A.; Jacob, R.; McNaughton, P. Actions of serum and plasma albumin on intracellular Ca2+ in human endothelial cells. J. Physiol. 504:315–326; 1997.

    Article  PubMed  CAS  Google Scholar 

  • Funke-Kaiser, H.; Orzechowski, H. D.; Richter, M.; Paul, M. Human endothelin-converting enzyme-1 beta mRNA expression is regulated by an alternative promoter. J. Cardiovasc. Pharmacol. 31 (Suppl 1):S7-S9; 1998.

    Article  PubMed  CAS  Google Scholar 

  • Harris, N. L.; Gang, D. L.; Quay, S. C.; Poppema, S.; Zamecnik, P. C.; Nelson-Rees, W. A.; O’Brien, S. I. Contamination of Hodgkin’s disease cell cultures. Nature (Lond) 289:228–230; 1981.

    Article  CAS  Google Scholar 

  • Hishinuma, A.; Yamanaka, T.; Kasai, S.; So, S.; Tsweng, C. C.; Bamba, N.; Ohtake, H.; Shimoda, S. Different growth control of the two human thyroid cell lines of adenomatous goiter and paillary carcinoma. Thyroid 5:41–46; 1995.

    Article  PubMed  CAS  Google Scholar 

  • Hughes, S. E. Functional characterization of the spontaneously transformed human umbilical vein endothelial cell line ECV304: use in an in vitro model of angiogenesis. Exp. Cell Res. 225:171–185; 1996.

    Article  PubMed  CAS  Google Scholar 

  • Kawakami, M.; Matsukuma, S.; Hara, M.; Hidaka, T.; Suzuki, K.; Kitani, A.; Hiroi, S.; Ishizuka, T.; Matsuki, Y.; Nakamura, H. A novel synoviocyte line (RAMAK-1) derived from a patient with rheumatoid arthritis. In Vitro Cell. Dev. Biol. 34A:85–87; 1998.

    Google Scholar 

  • Markovic, O.; Markovic, N. Cell cross-contamination in cell cultures: the silent and neglected danger. In Vitro Cell. Dev. Biol. 34:1–8; 1998.

    CAS  Google Scholar 

  • Nelson-Rees, W. A.; Flandermeyer, R. R. HeLa cultures defined. Science (Wash DC) 191:96–97; 1976.

    Article  CAS  Google Scholar 

  • Nelson-Rees, W. A.; Flandermeyer, R. R. Inter- and intraspecies contamination of human breast tumor cell lines HBC and BrCa5 and other cell cultures. Science (Wash DC) 195:1343–1344; 1977.

    Article  CAS  Google Scholar 

  • Nelson-Rees, W. A.; Flandermeyer, R. R.; Hawthorne, P. K. Banded marker chromosomes as indicators of intraspecies cellular contamination. Science (Wash DC) 184:1093–1096; 1974.

    Article  CAS  Google Scholar 

  • Pelloux, H.; Ricard, J.; Bracchi, V.; Markowicz, Y.; Verna, J. M.; Ambroise-Thomas, P. Tumor necrosis factor aIpha, interleukin 1 alpha, and interleukin 6 mRNA expressed by human astrocytoma cells after infection by three different strains of Toxoplasma gondii. Parasitol. Res. 80:271–276; 1994.

    Article  PubMed  CAS  Google Scholar 

  • Reid, Y.; McGuire, L.; O’Neill, K.; Macy, M.; Chen, T. R.; McClintock, P.; Dorotinsky, C.; Hay, R. Cell line cross-contamination of U-397. J. Leukocyte Biol. 57:804; 1995.

    PubMed  CAS  Google Scholar 

  • Takahashi, K.; Sawasaki, Y. Rare spontaneously transformed human endothelial cell line provides useful research tool. In Vitro Cell. Dev. Biol. 28A:380–382; 1992.

    Article  Google Scholar 

  • Takahashi, K.; Sawasaki, Y.; Hata, J.; Mukai, K.; Goto, T. Spontaneous transformation and immortalization of human endothelial cells. In Vitro Cell. Dev. Biol. 26:265–274; 1990.

    PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Human and Animal Cell Cultures, DSMZ-German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany

    Wilhelm G. Dirks, Roderick A. F. MacLeod & Hans G. Drexler M.D.

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  1. Wilhelm G. Dirks
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  2. Roderick A. F. MacLeod
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  3. Hans G. Drexler M.D.
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Dirks, W.G., MacLeod, R.A.F. & Drexler, H.G. ECV304 (endothelial) is really T24 (bladder carcinoma): Cell line cross-contamination at source. In Vitro Cell.Dev.Biol.-Animal 35, 558–559 (1999). https://doi.org/10.1007/s11626-999-0091-8

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  • DOI: https://doi.org/10.1007/s11626-999-0091-8

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