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In vitro antioxidant, brine shrimp lethality and antimicrobial activities of methanol and petroleum ether extracts of Cassia renigera fruit

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Abstract

Cassia renigera (C. renigera) is an evergreen tree (family Caesalpiniaceae) is locally known as Minjiri (Bengali). It is locally used as a medicinal plant. In this study we investigated antioxidant, brine shrimp lethality and antimicrobial activities of the fruit extracts of this plant using different in vitro assay models. Methanol extract showed better activities than petroleum ether extract in total phenol, total flavonoid and total antioxidant capacity assays. Besides, methanol extract also showed higher 1,1-diphenyl-2-picrylhydrazyl (DPPH) and Nitric oxide radical scavenging activities than petroleum ether extract. In cupric reducing and reducing power capacity assays methanol extract exhibited dose dependent reducing capacity. In brine shrimp lethality bioassay petroleum ether extract (LC50 of 89.385 μg/mL, χ2 = 215.968, p < 0.05) was found to be more potent than methanol extract (LC50 of 128.042 μg/mL, χ2 = 235.274, p < 0.05). In disk diffusion technique, it was observed that Gram positive bacteria are more susceptible than Gram negative bacteria. Both extracts showed broad spectrum antimicrobial activity. Methanol extract showed antibacterial activity against all tested microorganisms. The highest activity (minimum zone of inhibition 11.25 ± 0.35 mm) was exhibited by methanol extract against Bacillus cerus. No activity was observed against Escherichia coli and Shigella dysenteriae by petroleum ether extract. In a nutshell, it is suggested that the fruit can be used as a potential source for the above mentioned bioactivities. That is why extensive researches may be carried out to investigate the active principles responsible for these activities.

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References

  1. K.R. Prashant, J. Dolly, K.R. Singh, K.R. Gupta, G. Watal, Pharm. Biol. 46(12), 894–899 (2008)

    Article  Google Scholar 

  2. T.P. Devasagayam, J.C. Tilak, K.K. Boloor, K.S. Sane, S.S. Ghaskadbi, R.D. Lele, J. Assoc. Phys. India. 52, 794–804 (2004)

    CAS  Google Scholar 

  3. I. Karaman, F. Sahin, M. Gulluce, H. Ogutcu, M. Sengul, A. Adiguzel, J. Ethnopharmacol. 85(2–3), 231–235 (2003)

    Article  CAS  Google Scholar 

  4. A.H.M.M. Rahman, AJLS 1(3), 105–112 (2013)

    Article  Google Scholar 

  5. A. Ghani, Medicinal Plants of Bangladesh, 2nd edn. (The Asiatic Society of Bangladesh, Dhaka, 2003)

    Google Scholar 

  6. Y.S. Velioglu, G. Mazza, L. Gao, B.D. Oomah, J. Agric. Food Chem. 46, 4113–4117 (1998)

    Article  CAS  Google Scholar 

  7. S.Y. Wang, H. Jiao, J. Agric. Food Chem. 48, 5672–5676 (2000)

    Article  Google Scholar 

  8. P. Prieto, M. Pineda, M. Aguilar, Anal. Biochem. 269, 337–341 (1999)

    Article  CAS  Google Scholar 

  9. A. Braca, N.D. Tommasi, L.D. Bari, C. Pizza, M. Politi, I. Morelli, J. Nat. Prod. 64, 892–895 (2001)

    Article  CAS  Google Scholar 

  10. R. Govindarajan, S. Rastogi, M. Vijayakumar, A. Shirwaikar, A.K.S. Rawat, S. Mehrotra et al., Biol. Pharm. Bull. 26, 1424–1427 (2003)

    Article  CAS  Google Scholar 

  11. A. Resat, G. Kubilay, O. Mustafa, E.K. Saliha, J. Agric. Food Chem. 52, 7970–7981 (2004)

    Article  Google Scholar 

  12. M. Oyaizu, Jpn J Nutr. 44, 307–315 (1986)

    Article  CAS  Google Scholar 

  13. A.W. Bauer, W.M. Kirby, J.C. Sherris, M. Turck, Am. J. Clin. Pathol. 45(4), 493–496 (1966)

    CAS  Google Scholar 

  14. M.A. Lieberman, J. Chem. Ed. 76, 1689–1691 (1999)

    Article  CAS  Google Scholar 

  15. B.N. Meyer, N.R. Ferrigni, J.E. Putnam, L.B. Jacobsen, Planta. Med. 45, 31–34 (1982)

    Article  CAS  Google Scholar 

  16. K.C. Kokate, Practical pharmacognosy, 4th edn. (Vallabh Prakashan, Delhi, 1997), p. 218

    Google Scholar 

  17. M.A. Hossain, M.R. Nagooru, Pharmacogn. J. 3(24), 25–30 (2011)

    Article  CAS  Google Scholar 

  18. O.A. Aiyegoro, A.I. Okoh, BMC Complement. Altern. Med. 10, 21 (2010)

    Article  Google Scholar 

  19. T. Ozen, I. Turkekul, Pharmacogn. Mag. 6(22), 89–97 (2010)

    Article  CAS  Google Scholar 

  20. A. Turkoglu, M.E. Duru, N. Mercan, I. Kivark, K. Gezer, Food Chem. 101, 267–273 (2007)

    Article  CAS  Google Scholar 

  21. Y. You, X. Duan, X. Wei, X. Su, M. Zhao, J. Sun et al., Molecules 12(4), 842–852 (2007)

    Article  CAS  Google Scholar 

  22. G.D. Stoner, H. Mukhtar, J. Cell. Biochem. 22, 169–180 (1995)

    Article  CAS  Google Scholar 

  23. J. Moline, I.F. Bukharovich, M.S. Wolff, Med. Hypoth. 55(4), 306–309 (2000)

    Article  CAS  Google Scholar 

  24. G.G. Duthie, S.J. Duthei, J.A.M. Kyle, Nutr. Res. Rev. 13, 79–106 (2000)

    Article  CAS  Google Scholar 

  25. Y. Arai, S. Wantanabe, M. Kimira, K. Shimoi, J. Nutr. 130(9), 2243–2250 (2000)

    CAS  Google Scholar 

  26. O. Benavente-Garcia, J. Castillo, F.R. Marin, A. Ortuño, J.A. Del-Rio, J. Agric. Food Chem. 45, 4505–4515 (1997)

    Article  CAS  Google Scholar 

  27. P. Prieto, M. Pineda, M. Aguilar, Anal. Biochem. 269, 337–341 (1999)

    Article  CAS  Google Scholar 

  28. M. Suhaj, J. Food Comp. Anal. Discipl. 19, 531–537 (2006)

    Article  CAS  Google Scholar 

  29. A. Kumaran, R. Joel, Food Chem. 97, 109–114 (2006)

    Article  CAS  Google Scholar 

  30. M. Oktay, I. Gulcin, O.I. Kufrevioglu, Leb-Wissen Technol. 36, 263–271 (2003)

    Article  CAS  Google Scholar 

  31. C.W. Boone, G.J. Kelloff, W.E. Malone, Cancer Res. 50, 2–9 (1990)

    CAS  Google Scholar 

  32. R.A. Anderson, Integrative medicine [Internet], 2nd edn. (Elsevier, Philadelphia, 2007)

    Google Scholar 

  33. M. Déciga-Campos, I. Rivero-Cruz, M. Arriaga-Alba, G. Castañeda-Corral, G.E. Angeles-López, A. Navarrete, J. Ethnopharmacol. 110, 334–342 (2007)

    Article  Google Scholar 

  34. B. Ozcelik, M. Kartal, I. Orhan, Pharm. Biol. 49(4), 396–402 (2011)

    Article  CAS  Google Scholar 

  35. Y.C. Chang, K.W. Tai, F.M. Huang, M.F. Huang, J. Endod. 26(8), 440–443 (2000)

    Article  CAS  Google Scholar 

  36. Z. Zuraini, L. Yoga latha, S. Suryani, S. Sasidharan, Plant Kuala Lumpur. 80, 653–657 (2007)

    Google Scholar 

  37. D. Srinivasan, S. Nathan, T. Suresh, P. Lakshmana Perumalsamy, J. Ethnopharmacol. 74(3), 217–220 (2001)

    Article  CAS  Google Scholar 

  38. J.C.A. Tanaka, C.C. Silva, A.J.B. Oliveira, C.V. Nakamura, B.P. Dias Filho, Braz. J. Med. Biol. Res. 39(3), 387–391 (2006)

    Article  CAS  Google Scholar 

  39. I.K. Sawer, M.I. Berry, J.L. Ford, Lett. Appl. Microbial. 40, 24–29 (2005)

    Article  CAS  Google Scholar 

  40. J.N. Lisgarten, M. Coll, J. Portugal, C.W. Wright, J. Aymami, Nat. Struct. Biol. 9, 57–60 (2002)

    Article  Google Scholar 

  41. L. Dassonneville, A. Lansiaux, A. Wattelet, N. Wattez, C. Mathieu, M.S. Van et al., Eur. J. Pharmacol. 409, 9–18 (2000)

    Article  CAS  Google Scholar 

  42. K.T. Chung, T.Y. Wong, C.I. Wei, Y.W. Huang, Y. Lin, Crit. Rev. Food Sci. Nutr. 38(42), 1–464 (1998)

    Google Scholar 

  43. H. Akiyama, K. Fujii, O. Yamasaki, T. Oono, K. Iwatsuki, J. Antimicrob. Chemother. 48, 487–491 (2001)

    Article  CAS  Google Scholar 

  44. H.X. Xu, S.F. Lee, Phytother. Res. 15, 39–43 (2001)

    Article  Google Scholar 

  45. T.P. Cushnie, A.J. Lamb, Int. J. Antimicrob. Agents 26(5), 343–356 (2005)

    Article  CAS  Google Scholar 

  46. P. Mandal, S.P. SinhaBabu, N.C. Mandal, Fitoterapia 76(5), 462–465 (2005)

    Article  CAS  Google Scholar 

  47. P. Avato, R. Bucci, A. Tava, C. Vitali, A. Rosato, Z. Bialy et al., Phytother. Res. 20(6), 454–457 (2006)

    Article  CAS  Google Scholar 

  48. S.H. Taleb-Contini, M.J. Salvador, E. Watanabe, I.Y. Ito, D.C.R. Oliveira, Braz. J. Pharma. Sci. 39(4), 403–408 (2003)

    CAS  Google Scholar 

Download references

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Authors and Affiliations

  1. Department of Pharmacy, Jahangirnagar University, Savar, Dhaka, 1342, Bangladesh

    Md. Monir Hossain, Milon Mondal, Md. Parvez Hossain, Md. Shafiq Uddin, Nizam Uddin, Md. Mahadi Hasan, Md. Sazzad Hossain, A. F. M. Mahmudul Islam & Md. Sohel Rana

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  1. Md. Monir Hossain
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  2. Milon Mondal
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  3. Md. Parvez Hossain
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Correspondence to Md. Monir Hossain.

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Hossain, M.M., Mondal, M., Hossain, M.P. et al. In vitro antioxidant, brine shrimp lethality and antimicrobial activities of methanol and petroleum ether extracts of Cassia renigera fruit. Food Measure 9, 68–75 (2015). https://doi.org/10.1007/s11694-014-9211-6

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  • DOI: https://doi.org/10.1007/s11694-014-9211-6

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