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Establishment and Quality Assessment of Tissue Cultures in Glycyrrhiza uralensis Fisch.

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Abstract

In this study, seedling, callus, cell, and adventitious root of Glycyrrhiza uralensis Fisch. have been established. In order to find the best one for producing G. uralensis active constituents, triterpenoid saponins and flavonoids in native root and tissue cultures were determined, and the contents in different G. uralensis materials were analyzed using cluster analysis. The contents of triterpenoid saponins and glycyrrhizic acid in tissue cultures were much lower than that in native G. uralensis. The total flavonoids content we determined in adventitious root was 6.31 mg g−1, which was close to that of native root (9.82 mg g−1). Based on the cluster analysis, we found that G. uralensis cultures were not suitable for production of glycyrrhizic acid, while adventitious root had a greater capability of flavonoids production comparing to seedling, callus, and cell.

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Abbreviations

2,4-D:

2,4-Dichlorophenoxyacetic acid

KT:

Kinetin

NAA:

Naphthalene acetic acid

6-BA:

6-Benzylaminopurine

MS:

Murashige and Skoog

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Acknowledgments

This research was funded by Science and Technology Commission of Tianjin, China (09ZCKFSH01100).

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Authors and Affiliations

  1. School of Pharmaceutical Science and Technology, Tianjin University, Tianjin, 300072, China

    Juan Wang & Wenyuan Gao

  2. Institute of Biological Engineering of Traditional Chinese Medicine, Tianjin University of Science and Technology, Tianjin, 300457, China

    Liming Zhang

  3. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, 100700, China

    Luqi Huang

Authors
  1. Juan Wang
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  2. Wenyuan Gao
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  3. Liming Zhang
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  4. Luqi Huang
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Corresponding author

Correspondence to Wenyuan Gao.

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Wang, J., Gao, W., Zhang, L. et al. Establishment and Quality Assessment of Tissue Cultures in Glycyrrhiza uralensis Fisch.. Appl Biochem Biotechnol 169, 588–594 (2013). https://doi.org/10.1007/s12010-012-0012-2

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  • DOI: https://doi.org/10.1007/s12010-012-0012-2

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