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Triple Point-Mutants of Hypoxia-Inducible Factor-1α Accelerate In Vivo Angiogenesis in Bone Defect Regions

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Abstract

To investigate the functions of triple point-mutants of hypoxia-inducible factor 1α (HIF1α) in angiogenesis in bone defect regions under normoxic conditions. 1. Triple point-mutations (in amino acids 402, 564, and 803) in the HIF1α coding sequence (CDS) were induced by polymerase chain reaction. The triple mutant HIF1α (402/564/803) was inserted into the adenovirus pAdEasy-1 system for complete viral packaging and titer measurements. 2. For the in vitro experiment, rabbit bone marrow mesenchymal stem cells (MSCs) were divided into four experimental groups. The efficiency of infection was observed by the expression of human renilla reniformis green fluorescent protein (hrGFP). The HIF1α mRNA, protein and VEGF protein expression levels in infected cells in each experimental group were measured. 3. As in the in vivo experiment, the MSCs were divided into four groups and infected with the viral solutions from each complementary in vitro group and cultured under normoxic conditions. The MSCs were used as seed cells and transplanted into an apatite–wollastonite magnetic bioactive glass–ceramic (AW MGC) vector to construct artificial tissue-engineering scaffolds that were then implanted into the in vivo rabbit radial bone defect model. The animals from each group were killed 8 weeks after the surgery, and the tissues from the implantation region were harvested for the evaluation of the angiogenesis. 1. The 402,564, and 803 amino acids in CDS area were point mutated into alanine; three types of recombinant adenovirus were successfully constructed, packaged, and characterized. 2. The expression levels of HIF1α mRNA in A and B groups were significantly higher than those in the C and D groups (P < 0.05). The HIF1α and VEGF protein expression levels in A group were significantly higher than those in the other three groups (P < 0.05). 3. There was prominent angiogenesis in bone defect regions in group A animals. 1. Triple point-mutants of HIF1α efficiently expressed functional proteins under normoxic conditions. 2. Triple point-mutants HIF1α effectively promoted in vivo angiogenesis in bone defect regions.

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Acknowledgments

This project is funded by the Liaoning Affiliated Hospital Youth Science and Technology Startup Fund (FY2012-04).

Conflict of interest

The authors declare that they have no competing interests.

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Authors and Affiliations

  1. Central Laboratory, The First Affiliated Hospital of Liaoning Medical College, Jinzhou, China

    Chen Li

  2. Orthopedic Department, The First Affiliated Hospital of Liaoning Medical College, No. 2, Renming Street Wuduan, Guta District, Jinzhou, 121001, China

    Danping Liu & Zheng Zhang

  3. Pharmacology Department, The Basic Medicine College of Liaoning Medical College, Jinzhou, China

    Guoxian Wang

  4. Radiology Department, The First Affiliated Hospital of Liaoning Medical College, Jinzhou, China

    Na Xu

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  1. Chen Li
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  2. Danping Liu
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  3. Zheng Zhang
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Correspondence to Danping Liu.

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Li, C., Liu, D., Zhang, Z. et al. Triple Point-Mutants of Hypoxia-Inducible Factor-1α Accelerate In Vivo Angiogenesis in Bone Defect Regions. Cell Biochem Biophys 67, 557–566 (2013). https://doi.org/10.1007/s12013-013-9541-8

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  • DOI: https://doi.org/10.1007/s12013-013-9541-8

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