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Values of MMP-2 and MMP-9 in Tumor Tissue of Basal-Like Breast Cancer Patients

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Abstract

Gelatinase A (MMP-2) and gelatinase B (MMP-9) are proteolytic enzymes involved in process of tumor invasion, and they are considered as possible tumor markers in breast cancer patients. In this study, we measured activity of latent and active form of MMP-2 and MMP-9 in tumor and adjacent tissue of 60 breast cancer patients by SDS-PAGE zymography. The activity of both form of gelatinases significantly increased with each advancing clinical stage of disease. ProMMP-9 and aMMP-9 activity in tumor tissue shows a positive association with tumor size. Patients with lymph node involvement have higher proMMP-2, aMMP-2 and aMMP-9 activity than node negative patients. Steroid receptor-negative tumors had enhanced aMMP-2 and aMMP-9 activity. Patients with basal-like cancers had higher proMMP-2 tumor activity and aMMP-2 adjacent tissue activity compared to patients with luminal A tumors. Patients with negative hormone receptors are associated with increased activity of both form of gelatinases in adjacent tissue. Reported increased activity of MMP-2 in tumor and adjacent tissue of basal-like tumors implicates that MMP-2 might have a role in aggressive biology of basal-like cancers. Additional investigations regarding molecular pathways in adjacent tissue could give better insight into aggressive nature of basal-like carcinomas.

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Abbreviations

MMPs:

Matrix metalloproteinases

DAB:

3,3diaminobenzidine

ER:

Estrogen receptor

PR:

Progesterone receptor

HER2:

Human epidermal growth factor receptor 2

CK5/6:

Citokeratin 5 or 6

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Acknowledgments

This study was supported by the Grant of the Ministry of Science of Serbia No. 175056. The authors also thank Marija Jankovic for excellent technical assistance.

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Authors and Affiliations

  1. Department of Experimental Oncology, Institute of Oncology and Radiology of Serbia, Pasterova 14, 11 000, Belgrade, Serbia

    Sandra Radenkovic, Gordana Konjevic, Katarina Karadzic & Marina Nikitovic

  2. School of Medicine, University of Belgrade, Belgrade, Serbia

    Gordana Konjevic, Marina Nikitovic & Kristina Gopcevic

  3. School of Medicine, University of Kragujevac, Kragujevac, Serbia

    Vladimir Jurisic

  4. Department for Radiation Oncology and Diagnostics, Institute of Oncology and Radiology of Serbia, Pasterova 14, 11000, Belgrade, Serbia

    Sandra Radenkovic

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  1. Sandra Radenkovic
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  2. Gordana Konjevic
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  3. Vladimir Jurisic
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  4. Katarina Karadzic
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  5. Marina Nikitovic
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Correspondence to Vladimir Jurisic.

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Radenkovic, S., Konjevic, G., Jurisic, V. et al. Values of MMP-2 and MMP-9 in Tumor Tissue of Basal-Like Breast Cancer Patients. Cell Biochem Biophys 68, 143–152 (2014). https://doi.org/10.1007/s12013-013-9701-x

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