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Effect of Young Extrinsic Environment Stimulated by Hypoxia on the Function of Aged Tendon Stem Cell

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Abstract

Tendon stem cells (TSCs), recently identified as tendon cells, play an important role in maintaining the homeostasis of tendon tissue. Age-related decrease in the function of TSCs has been reported. Recent reports demonstrated that hypoxic condition is advantageous for efficient expansion of TSCs. Moreover, the impaired function of aged stem cells could be modulated by exposing them to a young environment. Therefore, we investigated the effects of hypoxic-conditioned culture medium (HCCM) from young TSCs on the proliferation, migration, senescence, and tenocyte phenotype of aged TSCs. TSCs were isolated, and the conditioned medium was collected. There were 4 groups: young TSCs, aged TSCs, aged TSCs + aged HCCM, and aged TSCs + young HCCM. The proliferative capacity, migration, β-galactosidase activity, and tenogenic differentiation potential of TSCs were assessed. Our results showed that HCCM enhanced the proliferation and migration potential of aged TSCs. Moreover, the senescence-associated β-galactosidase activity of aged TSCs was decreased by young HCCM. After being cultured in the young HCCM, the expressions of tenocyte-related genes in aged TSCs were significantly enhanced. Together, results of this study indicate that HCCM from young TSCs may represent an effective strategy to improve the impaired function of aged TSCs.

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Acknowledgments

This work was supported by Grants from the National Natural Sciences Foundation of China (Nos. 30901516, 81272049, 81150024), Reserve Talents of Universities Overseas Research Program of Heilongjiang, foundation for University Key Teacher of Heilongjiang Province of China (1253G042), and Natural Science Foundation of Heilongjiang (QC2011C049).

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The authors declare that they have no conflict of interests.

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Authors and Affiliations

  1. Department of Pediatric Surgery, Second Affiliated Hospital of Harbin Medical University, Harbin, 150086, China

    Dapeng Jiang, Zhitao Jiang, Yubo Zhang, Shuai Wang, Shulong Yang, Bo Xu, Mowen Yang & Zhaozhu Li

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  1. Dapeng Jiang
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  2. Zhitao Jiang
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  3. Yubo Zhang
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  8. Zhaozhu Li
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Correspondence to Zhaozhu Li.

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Dapeng Jiang and Zhitao Jiang contributed equally to this study.

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Jiang, D., Jiang, Z., Zhang, Y. et al. Effect of Young Extrinsic Environment Stimulated by Hypoxia on the Function of Aged Tendon Stem Cell. Cell Biochem Biophys 70, 967–973 (2014). https://doi.org/10.1007/s12013-014-0004-7

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  • DOI: https://doi.org/10.1007/s12013-014-0004-7

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