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SEPS1 Gene is Activated during Astrocyte Ischemia and Shows Prominent Antiapoptotic Effects

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Abstract

Contrarily to neurons, astrocytes can survive short periods of ischemia. We have searched for genes implicated in astrocyte resistance to ischemia using oxygen and glucose deprivation (OGD) as a stroke model. A RNA differential display approach uncovered the OGD induction of selenoprotein-S-encoding gene SEPS1. This endoplasmic reticulum (ER) resident protein is known to promote cell survival regulating the ER stress as well as inflammation. We found that suppression of SEPS1 by small interfering RNA severely increases astrocyte injure caused by OGD, suggesting that selenoprotein S protects astrocytes against ischemia. Our data also support that modulation of ER stress is implicated in this effect.

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Figure 1
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Abbreviations

CHOP:

CEBP homologous protein

ER:

endoplasmic reticulum

GPx:

glutathione peroxidase

MTT:

3-[4,5-dimethylthiazol-2yl-2,5-diphenyltetrazolium bromide

OGD:

oxygen and glucose deprivation

ROS:

reactive oxygen species

SEPS1:

selenoprotein S

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Acknowledgments

We thank Gema Rodriguez, Vanessa Guijarro, and Juana Rozalen for their expert technical assistance. This work was supported by grants from Spanish Ministry of Education and Science (BFU2006-14267) and Junta de Comunidades de Castilla-La Mancha (PAI05-017 and PCI-08-0101-8639) to S.C.

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Authors and Affiliations

  1. Pharmacology Unit, Facultad de Medicina, Departamento de Ciencias Médicas and Centro Regional de Investigaciones Biomédicas, Universidad de Castilla-La Mancha, 02006, Albacete, Spain

    Noelia Fradejas, María Dolores Pastor, Silvia Mora-Lee & Soledad Calvo

  2. Physiology Unit, Facultad de Medicina, Departamento de Ciencias Médicas and Centro Regional de Investigaciones Biomédicas, Universidad de Castilla-La Mancha, 02006, Albacete, Spain

    Pedro Tranque

  3. Facultad de Medicina, Universidad de Castilla La Mancha, Avda. de Almansa,14, 02006, Albacete, Spain

    Soledad Calvo

Authors
  1. Noelia Fradejas
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  2. María Dolores Pastor
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  3. Silvia Mora-Lee
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  4. Pedro Tranque
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  5. Soledad Calvo
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Correspondence to Soledad Calvo.

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Fradejas, N., Pastor, M.D., Mora-Lee, S. et al. SEPS1 Gene is Activated during Astrocyte Ischemia and Shows Prominent Antiapoptotic Effects. J Mol Neurosci 35, 259–265 (2008). https://doi.org/10.1007/s12031-008-9069-3

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  • DOI: https://doi.org/10.1007/s12031-008-9069-3

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