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Neuroprotective Effect of Lipoxin A4 Methyl Ester in a Rat Model of Permanent Focal Cerebral Ischemia

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Abstract

Neuroprotective effect of lipoxin A4 methyl ester (LXA4 ME) was tested in a rat model of permanent middle cerebral artery occlusion. LXA4 ME was administrated through intracerebroventricular injection immediately after middle cerebral artery was occluded. Administration of LXA4 ME ameliorated neurological deficit, reduced infarct volume, attenuated histological damage, and decreased number of apoptotic neuron induced by ischemic insult. These neuroprotective effects of LXA4 ME were associated with inhibition of neutrophil infiltration, lipid peroxidation, and astrocyte activation. In addition, LXA4 ME also attenuated proinflammatory cytokines (TNF-α and IL-1β) production. These data suggest that LXA4 ME protects neuron against permanent cerebral ischemia by inhibiting inflammatory responses.

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Acknowledgments

This work was supported by the Grant from National Natural Science Foundation of China (No. 30700784).

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Authors and Affiliations

  1. Department of Neurology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China

    Yan Wu

  2. Department of Anesthesiology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, No. 1277, Jiefang Avenue, 430022, Wuhan, China

    Yan Wu, Xi-Hong Ye, Pei-Pei Guo, Jie Wang, Shang-Long Yao & You Shang

  3. Department of Pathology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China

    San-Peng Xu

  4. Department of Critical Care Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, No. 1277, Jiefang Avenue, 430022, Wuhan, China

    Shi-Ying Yuan & You Shang

Authors
  1. Yan Wu
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  2. Xi-Hong Ye
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  3. Pei-Pei Guo
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  4. San-Peng Xu
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  5. Jie Wang
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  6. Shi-Ying Yuan
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  8. You Shang
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Corresponding authors

Correspondence to Shi-Ying Yuan or You Shang.

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Yan Wu and Xi-Hong Ye contributed equally to this work.

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Wu, Y., Ye, XH., Guo, PP. et al. Neuroprotective Effect of Lipoxin A4 Methyl Ester in a Rat Model of Permanent Focal Cerebral Ischemia. J Mol Neurosci 42, 226–234 (2010). https://doi.org/10.1007/s12031-010-9355-8

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  • DOI: https://doi.org/10.1007/s12031-010-9355-8

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