Abstract
Wilms’ tumor 1-associating protein (WTAP) interacts with the Wilms’ tumor 1 gene. Although originally classified as a tumor suppressor, WTAP was later found to be over-expressed in glioblastoma which is regarded as a grade IV astrocytoma. However, the expression in other glioma grades and the relationship between WTAP expression and the prognosis of glioma patients are still unknown. In this study, we investigated WTAP expression in 169 different types of glioma cases using western blot analysis and immunohistochemistry assay. Further, we evaluated the association of WTAP expression with clinicopathological characteristics using chi-square test and Spearman’s correlation test. We used univariate and multivariate Cox regression analyses to evaluate the independency of diferent WTAP expression. Then, the survival curves were calculated using the Kaplan-Meier method. Results showed that WTAP was over-expressed in glioma tissues, and the expression was closely correlated with glioma grade. Moreover, high WTAP expression was correlated with poor postoperative survival in glioma patients. WTAP may serve as a novel prognostic marker.
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Acknowledgments
This work is supported by grants from the Natural Science Foundation of China (81272564, 81372484, and 81573010), Liaoning Science and Technology Plan Project (No. 2015225007), Shenyang Science and Technology Plan Projects (Nos. F15-199-1-30 and F15-199-1-57) and outstanding scientific fund of Shengjing hospital (No. 201304).
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Written informed consent was obtained from the patients for publication of this study and any accompanying images. Research protocol was approved by the ethics committee of Shengjing Hospital of China Medical University.
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Xi, Z., Xue, Y., Zheng, J. et al. WTAP Expression Predicts Poor Prognosis in Malignant Glioma Patients. J Mol Neurosci 60, 131–136 (2016). https://doi.org/10.1007/s12031-016-0788-6
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DOI: https://doi.org/10.1007/s12031-016-0788-6