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Organotypic Hippocampal Slices as Models for Stroke and Traumatic Brain Injury

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Abstract

Organotypic hippocampal slice cultures (OHSCs) have been used as a powerful ex vivo model for decades. They have been used successfully in studies of neuronal death, microglial activation, mossy fiber regeneration, neurogenesis, and drug screening. As a pre-animal experimental phase for physiologic and pathologic brain research, OHSCs offer outcomes that are relatively closer to those of whole-animal studies than outcomes obtained from cell culture in vitro. At the same time, mechanisms can be studied more precisely in OHSCs than they can be in vivo. Here, we summarize stroke and traumatic brain injury research that has been carried out in OHSCs and review classic experimental applications of OHSCs and its limitations.

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Acknowledgments

This work was supported by an AHA Mid-Atlantic Affiliate Grant-in-Aid 13GRNT15730001, NIH grants R01NS078026 and R01AT007317 (JW), and an AHA Mid-Atlantic Affiliate Postdoctoral Fellowship Award (XH).

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    1. Department of Anesthesiology and Critical Care Medicine, The Johns Hopkins University School of Medicine, 720 Rutland Ave, Ross Bldg 370B, Baltimore, MD, 21205, USA

      Qian Li, Xiaoning Han & Jian Wang

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    1. Qian Li
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    Correspondence to Jian Wang.

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    Qian Li and Xiaoning Han contributed equally to this work.

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    Li, Q., Han, X. & Wang, J. Organotypic Hippocampal Slices as Models for Stroke and Traumatic Brain Injury. Mol Neurobiol 53, 4226–4237 (2016). https://doi.org/10.1007/s12035-015-9362-4

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