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The cloning and expression of renalase and the preparation of its monoclonal antibody

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Abstract

To obtain the recombination protein of renalase and prepare the monoclonal antibody, the human renalase gene was amplified from human kidney by specific primer and cloned the DNA fragments into the pET22b. After verification of the positive clones, the gene was transformed to E. coli BL21 to express the protein with 6His on C terminal. The Balb/c mouse was immunized with the purified protein to prepare the monoclonal antibody by hybidoma technique. The renalase protein was reconstructed and 2 strains of the hybidoma which can stable secrete renalase were obtained. The monoclonal antibody can both react with the both recombinant and human serum renalase.

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Authors and Affiliations

  1. Department of Nephrology, the 6th People’s Hospital Affiliated to Shanghai Jiaotong University, Shanghai, 200233, China

    Feng Wang  (王 锋), Nian-song Wang  (汪年松) & Hai-yan Xiang  (向海燕)

  2. the Australian School of Advanced Medicine, Macquarie University, Sydney, NSW, Australia

    Tao Xing  (邢 涛)

  3. School of Life Science & Biotechnology, Shanghai Jiaotong University, Shanghai, 200240, China

    Yang Cao  (曹 阳)

Authors
  1. Feng Wang  (王 锋)
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  2. Nian-song Wang  (汪年松)
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  3. Tao Xing  (邢 涛)
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  4. Yang Cao  (曹 阳)
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  5. Hai-yan Xiang  (向海燕)
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Corresponding author

Correspondence to Nian-song Wang  (汪年松).

Additional information

Foundation item: the Scientific Research Project of Shanghai Municipal Health Bureau (2008Y034), and the Natural Scientific Research Project of Shanghai (05ZR14086)

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Wang, F., Wang, Ns., Xing, T. et al. The cloning and expression of renalase and the preparation of its monoclonal antibody. J. Shanghai Jiaotong Univ. (Sci.) 14, 376–379 (2009). https://doi.org/10.1007/s12204-009-0376-9

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  • DOI: https://doi.org/10.1007/s12204-009-0376-9

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