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KH-type splicing regulatory protein is regulated by nuclear factor-κB signaling to mediate innate immunity in Caco-2 cells infected by Salmonella enteritidis

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Abstract

Salmonella enteritidis infection occurs in enterogenous diseases, such as gastroenteritis and parenteral focal infection, which often involve inflammation of intestinal epithelial cells. The nuclear factor kappa B (NF-κB) pathway participates in the innate immune response to many gram-negative pathogenic bacteria and initiates inflammation in epithelial cells. KH-type splicing regulatory protein (KSRP) is a multi-domain RNA-binding protein that recruits the exosome-containing mRNA degradation complex to mRNAs coding for inflammatory response factors. However, it remains unclear whether KSRP is regulated by NF-κB signaling pathway in response to S. enteritidis infection and affects the development of inflammation. Accordingly, in this study, we investigated the role of KSRP in mediating the response to S. enteritidis in Caco-2 cells. The data revealed that S. enteritidis infection decreased KSRP expression, which was suppressed by blocking the NF-κB pathway. Additionally, S. enteritidis infection significantly increased the expression of inducible nitric oxide synthase and cyclooxygenase-2. Overexpression of KSRP reduced the expression levels of inflammatory factors in Caco-2 cells. KSRP was regulated by the NF-κB signaling pathway and participated in mediating the innate immune response to S. enteritidis infection in Caco-2 cells, and KSRP acted as a negative regulator of inflammatory gene expression.

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Acknowledgements

We thank Prof. Xianming Chen (Creighton University Medical Center, NE, USA) for providing the plasmid.

Funding

This work was supported by the National Natural Science Foundation of China (31270175); the Program for New Century Excellent Talents in University (NCET-13-0397); and the Fundamental Research Funds for the Central Universities (2013SCU04B14).

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Author notes

  1. Yuanyang Nie and Mei Cao contributed equally to this work.

Authors and Affiliations

  1. Key Laboratory of Biological Resource and Ecological Environment of Chinese Education Ministry, College of Life Sciences, Sichuan University, No.24 South Section 1, Yihuan Road, Chengdu, 610064, People’s Republic of China

    Yuanyang Nie, Daoyan Wu, Ningzhe Li, Jingshan Peng, Sijun Yi, Xiaofan Yang & Jian Zhao

  2. Core Laboratory, School of Medicine, Sichuan Provincial People’s Hospital Affiliated to University of Electronic Science and Technology of China, Chengdu, 610072, People’s Republic of China

    Mei Cao, Mao Zhang & Guoku Hu

  3. Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, NE, 18678, USA

    Guoku Hu

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  1. Yuanyang Nie
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  10. Jian Zhao
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Correspondence to Jian Zhao.

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Nie, Y., Cao, M., Wu, D. et al. KH-type splicing regulatory protein is regulated by nuclear factor-κB signaling to mediate innate immunity in Caco-2 cells infected by Salmonella enteritidis. Folia Microbiol 63, 669–676 (2018). https://doi.org/10.1007/s12223-018-0606-3

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