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Proteomic analyses of the shrimp white spot syndrome virus

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Virologica Sinica

Abstract

White spot syndrome virus (WSSV), a unique member within the virus family Nimaviridae, is the most notorious aquatic virus infecting shrimp and other crustaceans and has caused enormous economic losses in the shrimp farming industry worldwide. Therefore, a comprehensive understanding of WSSV morphogenesis, structural proteins, and replication is essential for developing prevention measures of this serious parasite. The viral genome is approximately 300kb and contains more than 180 open reading frames (ORF). However, most of proteins encoded by these ORF have not been characterized. Due to the importance of WSSV structural proteins in the composition of the virion structure, infection process and interaction with host cells, knowledge of structural proteins is essential to understanding WSSV entry and infection as well as for exploring effective prevention measures. This review article summarizes mainly current investigations on WSSV structural proteins including the relative quantities, localization, function and protein-protein interactions. Traditional proteomic studies of 1D or 2D gel electrophoresis separations and mass spectrometry (MS) followed by database searches have identified a total of 39 structural proteins. Shotgun proteomics and iTRAQ were initiated to identify more structural proteins. To date, it is estimated that WSSV is assembled by at least 59 structural proteins, among them 35 are defined as the envelope fraction (including tegument proteins) and 9 as nucleocapsid proteins. Furthermore, the interaction within several major structural proteins has also been investigated. This identitification and characterization of WSSV protein components should help in the understanding of the viral assembly process and elucidate the roles of several major structural proteins.

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References

  1. Bigot Y, Stasiak K, Rouleux-Bonnin F, et al. 2000. Characterization of repetitive DNA regions and methylated DNA in ascovirus genomes. J Gen Virol, 81:3073–3082.

    PubMed  CAS  Google Scholar 

  2. Carstens E B, Wu Y. 2007. No single homologous repeat region is essential for DNA replication of the baculovirus Autographa californica multiple nucleopolyhedrovirus. J Gen Virol, 88: 114–122.

    Article  PubMed  CAS  Google Scholar 

  3. Chang P S, Chen H C, Wang Y C. 1998. Detection of white spot syndrome associated baculovirus in experimentally infected wild shrimp, crab and lobsters by in situ hybridization. Aquaculture, 164: 233–242.

    Article  CAS  Google Scholar 

  4. Chang P S, Lo C F, Wang Y C, et al. 1996. Identification of white spot syndrome associated baculovirus (WSBV) target organs in the shrimp Penaeus monodon by in situ hybridization. Dis Aquat Org, 27: 131–139.

    Article  Google Scholar 

  5. Chen J, Li Z, Hew C L. 2007. Characterization of a novel envelope protein WSV010 of shrimp white spot syndrome virus and its interaction with a major viral structural protein VP24. Virology, 364: 208–213.

    Article  PubMed  CAS  Google Scholar 

  6. Chen L, Lo C F, Chiu Y L, et al.. 2000. Experimental infection of white spot syndrome virus (WSSV) in benthic larvae of mud crab Scylla serrata. Dis Aquat Org, 40: 157–161.

    Article  PubMed  CAS  Google Scholar 

  7. Chen L L, Leu J H, Huang C J, et al. 2002. Identification of a nucleocapsid protein (VP35) gene of shrimp white spot syndrome virus and characterization of the motif important for targeting VP35 to the nuclei of transfected insect cells. Virology, 293: 44–53.

    Article  PubMed  CAS  Google Scholar 

  8. Chen L L, Wang H C, Huang C J, et al. 2002. Transcriptional analysis of the DNA polymerase gene of shrimp white spot syndrome virus. Virology, 301: 136–147.

    Article  PubMed  CAS  Google Scholar 

  9. Cochran M A, Faulkner P. 1983. Localization of homologous DNA sequences interspersed at five regions in the baculovirus AcMNPV genome. J Virol, 45:961–970.

    PubMed  CAS  Google Scholar 

  10. Corbel V, Zuprizal Z, Shi Z, et al. 2000. Experimental infection of European crustaceans with white spot syndrome virus (WSSV). J Fish Dis, 24: 377–382.

    Article  Google Scholar 

  11. de Castro E, Sigrist C J A, Gattiker A, et al. 2006. ScanProsite: detection of PROSITE signature matches and ProRule-associated functional and structural residues in proteins. Nucleic Acids Res, 34: 362–365

    Article  Google Scholar 

  12. Durand S, Lightner D V, Redman R M, et al. 1997. Ultrastructure and morphogenesis of White spot syndrome baculovirus (WSSV). Dis Aquat Org, 29: 205–211.

    Article  Google Scholar 

  13. Flegel T W. 1997. Major viral diseases of the black tiger prawn (Penaeus monodon) in Thailand. World J Microbiol Biotechnol, 13: 433–442.

    Article  Google Scholar 

  14. Guarino L A, Summers M D. 1986. Functional mapping of a trans-activating gene required for expression of a baculovirus delayed-early gene. J Virol, 57: 563–571.

    PubMed  CAS  Google Scholar 

  15. Hameed A S, Yoganadhan K, Sathish S, et al. 2001. White spot syndrome virus (WSSV) in two species of freshwater crabs White amanitaand P. pulvinata. Aquaculture, 201: 179–186.

    Article  Google Scholar 

  16. Hameed A S, Anilkumar M, et al. 1998. Studies on the pathogenicity of systemic ectodermal and mesodermal baculovirus and its detection in shrimp by immunological methods. Aquaculture, 160: 31–45.

    Article  Google Scholar 

  17. Huang C, Zhang X, Lin Q, et al. 2002. Characterization of a novel envelope protein (VP281) of shrimp white spot syndrome virus by mass spectrometry. J Gen Virol, 83: 2385–2392.

    PubMed  CAS  Google Scholar 

  18. Huang C, Zhang X, Lin Q, et al. 2002. Proteomic analysis of shrimp white spot syndrome viral proteins and characterization of a novel envelope protein VP466. Mol Cell Proteomics, 1: 223–231.

    Article  PubMed  CAS  Google Scholar 

  19. Huang R, Xie Y, Zhang J, et al. 2005. A novel envelope protein involved in White spot syndrome virus infection. J Gen Virol, 86: 1357–1361.

    Article  PubMed  CAS  Google Scholar 

  20. Kool M, Van den Berg PMMM, Tramper J, et al. 1993. Location of two putative origins of DNA replication of Autographa californica nuclear polyhedrosis virus. Virology, 192: 94–101.

    Article  PubMed  CAS  Google Scholar 

  21. Leu J H, Tsai J M, Wang H C, et al. 2005. The unique stacked rings in the nucleocapsid of the white spot syndrome virus virion are formed by the major structural protein VP664, the largest viral structural protein ever found. J Virol, 79: 140–149.

    Article  PubMed  CAS  Google Scholar 

  22. Li H, Zhu Y, Xie X, et al. 2006. Identification of a novel envelope protein (VP187) gene from shrimp white spot syndrome virus. Virus Res, 115: 76–84.

    Article  PubMed  CAS  Google Scholar 

  23. Li L, Lin S, Yang F. 2006. Characterization of an envelope protein (VP110) of White spot syndrome virus. J Gen Virol, 87: 1909–1915.

    Article  PubMed  CAS  Google Scholar 

  24. Li L, Xie X, Yang F. 2005. Identification and characterization of a prawn white spot syndrome virus gene that encodes an envelope protein VP31. Virology, 340: 125–132.

    Article  PubMed  CAS  Google Scholar 

  25. Li L J, Yuan J F, Cai C A, et al. 2006. Multiple envelope proteins are involved in white spot syndrome virus (WSSV) infection in crayfish. Arch Virol, 151: 1309–1317.

    Article  PubMed  CAS  Google Scholar 

  26. Li Q, Chen Y, Yang F. 2004. Identification of a collagen-like protein gene from white spot syndrome virus. Arch Virol, 149: 215–223.

    Article  PubMed  CAS  Google Scholar 

  27. Li Z, Lin Q, Chen J, et al. 2007. Shotgun Identification of the Structural Proteome of Shrimp White Spot Syndrome Virus and iTRAQ Differentiation of Envelope and Nucleocapsid Subproteomes. Mol Cell Proteomics, 6: 1609–1620.

    Article  PubMed  CAS  Google Scholar 

  28. Lightner D V. 1996. A handbook of pathology and diagnostic procedures for diseases of penaeid shrimp. In: Special publication of the World Aquaculture Society. LA: Baton Rouge, p304.

    Google Scholar 

  29. Liu W J, Yu H T, Peng S E, et al. 2001. Cloning, characterization, and phylogenetic analysis of a shrimp white spot syndrome virus gene that encodes a protein kinase. Virology, 289: 362–377.

    Article  PubMed  CAS  Google Scholar 

  30. Lo C F, Ho C H, Peng S E, et al. 1996. White spot syndrome associated virus (WSBV) detected in cultured and captured shrimp, crabs and other arthropods. Dis Aquat. Org, 27:215–225.

    Article  Google Scholar 

  31. Lo C F, Ho C H, Chen C H, et al. 1997. Detection and tissue tropism of White spot syndrome baculovirus (WSBV) in captured brooders of Penaeus monodon with a special emphasis on reproductive organs. Dis Aquat Org, 30: 53–72.

    Article  Google Scholar 

  32. Lo C F, Hsu H C, Tsai M F, et al. 1999. Specific genomic DNA fragment analysis of different geographical clinical samples of shrimp white spot syndrome virus. Dis Aquat Org, 35: 175–185.

    Article  CAS  Google Scholar 

  33. Lotz J M. 1997. Viruses, Biosecurity and Specific Pathogen Free Stocks In Shrimp Aquaculture. World J Microbiol Biotechnol, 13: 405–413.

    Article  Google Scholar 

  34. Mayo M A. 2002. A summary of taxonomic changes recently approved by ICTV. Arch Virol, 147:1655–1663.

    Article  PubMed  CAS  Google Scholar 

  35. Momoyama K, Hiraoka M, Nakano H, et al. 1994. Mass mortalities of cultured kuruma shrimp Penaeus Japonicus, in Japan in 1993: histopathological study. Fish Path, 29: 141–148.

    Google Scholar 

  36. Nadala E C B, Tapay L M, Loh P C. 1998. Characterization of a non-occluded baculovirus-like agent pathogenic to penaeid shrimp. Dis Aquat Org, 33:221–229.

  37. Pandey A, Mann M. 2000. Proteomics to study genes and genomes. Nature, 405: 837–846.

    Article  PubMed  CAS  Google Scholar 

  38. Pearson M, Bjornson R, Pearson G, et al.. 1992. The Autographa californica baculovirus genome: evidence for multiple replication origins. Science, 257:1382–1384.

    Article  PubMed  CAS  Google Scholar 

  39. Sritunyalucksana K, Wannapapho W, Lo C F, et al. 2006. PmRab7 is a VP28-binding protein involved in white spot syndrome virus infection in shrimp. J Virol, 80: 10734–10742.

    Article  PubMed  CAS  Google Scholar 

  40. Tang X, Wu J, Sivaraman J, et al. 2007. Crystal structures of major envelope proteins VP26 and VP28 from white spot syndrome virus shed light on their evolutionary relationship. J Virol, 81: 6709–6717.

    Article  PubMed  CAS  Google Scholar 

  41. Tsai J M, Wang H C, Leu J H, et al. 2004. Genomic and proteomic analysis of thirty-nine structural proteins of shrimp white spot syndrome virus. J Virol, 78: 11360–11370.

    Article  PubMed  CAS  Google Scholar 

  42. Tsai J M, Wang H C, Leu J H, et al. 2006. Identification of the nucleocapsid, tegument, and envelope proteins of the shrimp white spot syndrome virus virion. J Virol, 80:3021–3029.

    Article  PubMed  CAS  Google Scholar 

  43. Tsai J M, Wang H C, Leu J H, et al. 2000. Transcriptional analysis of the ribonucleotide reductase genes of shrimp White spot syndrome virus. Virology, 277: 92–99.

    Article  PubMed  CAS  Google Scholar 

  44. Tsai M F, Yu H T, Tzeng H F, et al. 2000. Identif ication and characterization of a shrimp white spot syndrome virus (WSSV) gene that encodes a novel chimeric polypeptide of cellular-type thymidine kinase and thymidylate kinase. Virology, 277: 100–110.

    Article  PubMed  CAS  Google Scholar 

  45. van Hulten M C, Goldbach R W, Vlak J M. 2000. Three functionally diverged major structural proteins of white spot syndrome virus evolved by gene duplication. J Gen Virol, 81: 2525–2529.

    PubMed  Google Scholar 

  46. van Hulten M C, Reijns M, Vermeesch A M, et al. 2002. Identification of VP19 and VP15 of white spot syndrome virus (WSSV) and glycosylation status of the WSSV major structural proteins. J Gen Virol, 83: 257–265.

    PubMed  Google Scholar 

  47. van Hulten M C, Tsai M F, Schipper C A, et al. 2000. Analysis of a genomic segment of white spot syndrome virus of shrimp containing ribonucleotide reductase genes and repeat regions. J Gen Virol, 81: 307–316.

    PubMed  Google Scholar 

  48. van Hulten M C, Westenberg M, Goodall S D, et al.. 2000. Identification of two major virion protein genes of white spot syndrome virus of shrimp. Virology, 266: 227–236.

    Article  PubMed  Google Scholar 

  49. van Hulten M C, Witteveldt J, Peters S, et al. 2001. The white spot syndrome virus DNA genome sequence. Virology, 286:7–22.

    Article  PubMed  Google Scholar 

  50. van Hulten M C, Witteveldt J, Snippe M, et al. 2001. White spot syndrome virus envelope protein VP28 is involved in the systemic infection of shrimp. Virology, 285: 228–233.

    Article  PubMed  Google Scholar 

  51. Vlak J M, Bonami J R, Flegel T W, et al. 2005. Nimaviridae. In: VIIIth Report of the International Committee on Taxonomy of Viruses (Fauquet C M, Mayo M A, Maniloff J, et al. ed), Amsterdam: Elsevier, 187–192.

    Google Scholar 

  52. Wang C H, Lo C F, Leu J H, et al. 1995. Purification and genomic analysis of baculovirus associated with white spot syndrome (WSBV) of Penaeus monodon. Dis Aquat Organ, 23:239–242.

    Article  Google Scholar 

  53. Wang Q, Poulos B T, Lightner D V. 2000. Protein analysis of geographic isolates of shrimp white spot syndrome virus. Arch Virol, 145:263–274.

    Article  PubMed  CAS  Google Scholar 

  54. Witteveldt J, van Hulten M C, Vlak J M. 2001. Identification and phylogeny of a non-specific endonuclease gene of white spot syndrome virus of shrimp. Virus Genes, 23: 331–237.

    Article  PubMed  CAS  Google Scholar 

  55. Witteveldt J, Vermeesch A M, Langenhof M, et al. 2005. Nucleocapsid protein VP15 is the basic DNA binding protein of white spot syndrome virus of shrimp. Arch Virol, 150: 1121–1333.

    Article  PubMed  CAS  Google Scholar 

  56. Witteveldt J, Vermeesch A M, Langenhof M, et al. 2005. Nucleocapsid protein VP15 is the basic DNA binding protein of white spot syndrome virus of shrimp. Arch Virol, 150: 1121–1133.

    Article  PubMed  CAS  Google Scholar 

  57. Wongteerasupaya C, Vickers J E, Sriurairatana S, et al. 1995. A non-occluded, systemic baculovirus that occurs in cells of ectodermal and mesodermal origin and causes high mortality in the black tiger prawn Penaeus monodon. Dis Aquat Org, 21: 69–77.

    Article  Google Scholar 

  58. Wu W, Wang L, Zhang X. 2005. Identification of white spot syndrome virus (WSSV) envelope proteins involved in shrimp infection. Virology, 332: 578–583.

    Article  PubMed  CAS  Google Scholar 

  59. Xie X, Xu L, Yang F. 2006. Proteomic analysis of the major envelope and nucleocapsid proteins of white spot syndrome virus. J Virol, 80: 10615–10623.

    Article  PubMed  CAS  Google Scholar 

  60. Xie X, Yang F. 2006. White spot syndrome virus VP24 interacts with VP28 and is involved in virus infection. J Gen Virol, 87: 1903–1908.

    Article  PubMed  CAS  Google Scholar 

  61. Yang F, He J, Lin X, et al. 2001. Complete genome sequence of the shrimp white spot bacilliform virus. J Virol, 75:11811–11820.

    Article  PubMed  CAS  Google Scholar 

  62. Yi G, Wang Z, Qi Y, et al. 2004. Vp28 of shrimp white spot syndrome virus is involved in the attachment and penetration into shrimp cells. J Biochem Mol Biol, 37: 726–734.

    PubMed  CAS  Google Scholar 

  63. Zhang X, Huang C, Tang X, et al. 2004. Identification of structural proteins from shrimp white spot syndrome virus (WSSV) by 2DE-MS. Proteins, 55: 229–235.

    Article  PubMed  CAS  Google Scholar 

  64. Zhang X, Huang C, Xu X, et al. 2002. Identification and localization of a prawn white spot syndrome virus gene that encodes an envelope protein. J Gen Virol, 83: 1069–1674.

    PubMed  CAS  Google Scholar 

  65. Zhang X, Huang C, Xu X, et al. 2002. Transcription and identification of an envelope protein gene (p22) from shrimp white spot syndrome virus. J Gen Virol, 83: 471–477.

    PubMed  CAS  Google Scholar 

  66. Zhang X, Xu X, Hew C L. 2001. The structure and function of a gene encoding a basic peptide from prawn white spot syndrome virus. Virus Res, 79: 137–144.

    Article  PubMed  CAS  Google Scholar 

  67. Zhu Y, Xie X, Yang F. 2005. Transcription and identification of a novel envelope protein (VP124) gene of shrimp white spot syndrome virus. Virus Res, 113: 100–106.

    Article  PubMed  CAS  Google Scholar 

  68. Zhu Y B, Li H Y, Yang F. 2006. Identification of an envelope protein (VP39) gene from shrimp white spot syndrome virus. Arch Virol, 151: 71–82.

    Article  PubMed  CAS  Google Scholar 

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  1. State key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences (CAS), Wuhan, 430071, China

    Yan-wei Tan & Zheng-li Shi

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Tan, Yw., Shi, Zl. Proteomic analyses of the shrimp white spot syndrome virus. Virol. Sin. 23, 157–166 (2008). https://doi.org/10.1007/s12250-008-2924-0

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  • DOI: https://doi.org/10.1007/s12250-008-2924-0

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