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Down-regulation of HIV-1 infection by inhibition of the MAPK signaling pathway

  • Published:
Virologica Sinica

Abstract

The human immunodeficiency virus type 1 (HIV-1) can interact with and exploit the host cellular machinery to replicate and propagate itself. Numerous studies have shown that the Mitogen-activated protein kinase (MAPK) signal pathway can positively regulate the replication of HIV-1, but exactly how each MAPK pathway affects HIV-1 infection and replication is not understood. In this study, we used the Extracellular signal-regulated kinase (ERK) pathway inhibitor, PD98059, the Jun N-terminal kinase (JNK) pathway inhibitor, SP600125, and the p38 pathway inhibitor, SB203580, to investigate the roles of these pathways in HIV-1 replication. We found that application of PD98059 results in a strong VSV-G pseudotyped HIV-1NL4-3 luciferase reporter virus and HIV-1NL4-3 virus inhibition activity. In addition, SB203580 and SP600125 also elicited marked VSV-G pseudotyped HIV-1NL4-3 luciferase reporter virus inhibition activity but no HIV-1NL4-3 virus inhibition activity. We also found that SB203580 and SP600125 can enhance the HIV-1 inhibition activity of PD98059 when cells were treated with all three MAPK pathway inhibitors in combination. Finally, we show that HIV-1 virus inhibition activity of the MAPK pathway inhibitors was the result of the negative regulation of HIV-1 LTR promoter activity.

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Authors and Affiliations

  1. The State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China

    Jian Gong, Xi-hui Shen, Chao Chen, Hui Qiu & Rong-ge Yang

  2. School of Life Science, Huzhou Teachers College, Huzhou, 313000, China

    Jian Gong

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  1. Jian Gong
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  2. Xi-hui Shen
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  3. Chao Chen
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  4. Hui Qiu
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  5. Rong-ge Yang
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Correspondence to Rong-ge Yang.

Additional information

Foundation items: Key Projects in the National Science and Technology Pillar Program during the Eleventh Five-Year Plan Period of China (2008ZX10001-002); Major Science and Technology Innovation Cross Project of the Chinese Academy of Sciences (KSCX1-YW-10).

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Gong, J., Shen, Xh., Chen, C. et al. Down-regulation of HIV-1 infection by inhibition of the MAPK signaling pathway. Virol. Sin. 26, 114–122 (2011). https://doi.org/10.1007/s12250-011-3184-y

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  • DOI: https://doi.org/10.1007/s12250-011-3184-y

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