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Phytochemical analysis and antinociceptive properties of the seeds of Garcinia achachairu

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Abstract

In a search for new and effective analgesic substances from the Brazilian biodiversity, the present study evaluates the chemical composition and antinociceptive potential of the methanol extract and a pure compound obtained from the seeds of Garcinia achachairu Rusby (Clusiaceae). The methanolic seed extract was directly subjected to purification by column chromatography and the purification was monitored by thin-layer chromatography. The main isolated compound was identified as Guttiferone A by comparison of conventional spectroscopic data (IR, NMR-1H and 13C) to the literature data which was isolated for the first time from this plant. When evaluated in the acetic acid-induced nociception model in mice, the methanolic seed extract had an ID50 (Inhibitory dose) of 13.1 (11.23–14.91) mg/kg and a maximal inhibition of 72 ± 4%. In the same model, Guttiferone A had an ID50 of 4.54 (3.29–6.24) mg/kg and a maximal inhibition of 73 ± 5%. The methanolic seed extract and Guttiferone A were also active in pain models induced by formalin, capsaicin, glutamate and carrageenan. These data suggest that the antinociceptive effect of Guttiferone A partly depends on its interference with the synthesis or activity of the cytokine TNF-α, the keratinocyte-derived chemokine KC, and/or PGE2. These data support, at least in part, the use of G. achachairu in folk medicine and suggest that this plant is an important source of compounds with a suitable profile for development as new and effective medicinal agents to treat pain processes.

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Authors and Affiliations

  1. Programa de Mestrado em Ciências Farmacêuticas e Núcleo de Investigações Químico-Farmacêuticas (NIQFAR), Universidade do Vale do Itajaí, Rua Uruguai, 458, 88302-202, Itajaí, SC, Brazil

    Marlova Manhabosco Dal Molin, Suellen Silva, Douglas Rafael Alves, Nara Lins Meira Quintão, Franco Delle Monache, Valdir Cechinel Filho & Rivaldo Niero

  2. Programa de Mestrado em Ciências Farmacêuticas, Núcleo de Investigações Químico-Farmacêuticas (NIQFAR), Universidade do Vale do Itajaí — UNIVALI, Rua Uruguai, 458 Bloco 27 Sala 20, ZIP CODE: 88302-202, Itajaí, SC, Brazil

    Rivaldo Niero

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  1. Marlova Manhabosco Dal Molin
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  2. Suellen Silva
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  3. Douglas Rafael Alves
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Dal Molin, M.M., Silva, S., Alves, D.R. et al. Phytochemical analysis and antinociceptive properties of the seeds of Garcinia achachairu . Arch. Pharm. Res. 35, 623–631 (2012). https://doi.org/10.1007/s12272-012-0405-3

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