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Detection of Pseudomonas aeruginosa carried a new array of gene cassettes within class 1 integron isolated from a teaching hospital in Nanjing, China

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Abstract

We report here novel array of gene cassettes found in single variable region of class 1 integron disseminated in Pseudomonas aeruginosa isolated from a teaching hospital in Nanjing, Jiangsu Province, China. 29 of 47 (61%) P. aeruginosa strains were confirmed haboured class 1 integron, and all the positive strains have the same variable region confirmed by PCR and RFLP methods. The variable region contained an unreported order of four gene cassettes aac(6′)-II-aadA13-cmlA8-oxa-10. Of those, cmlA8 gene was a variant of cmlA5 encoding non-enzymatic protein which putatively confer resistance to chloramphenicol. Susceptibility testing revealed multidrug-resistant mechanisms were involved in the class 1 integron positive clinical isolates. And the class 1 integron located on an about 15 kb transferable plasmid was certified by conjugation experiment and plasmid DNA analysis. The macro restriction profile indicated those clinical strains were clonally related.

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References

  • Bissonnette, L., S. Champetier, J.P. Buisson, and P.H. Roy. 1991. Characterization of the nonenzymatic chloramphenicol resistance (cmlA) gene of the 1n4 integron of Tn1696: similarity of the product to transmembrane transport proteins. J. Bacteriol. 173, 4493–4502.

    PubMed  CAS  Google Scholar 

  • Clinical and Laboratory Stardards Institute. 2006. Performance standards for antimicrobial susceptibility testing; sixteenth informational supplement. CLSI document. M100-S16., Wayne, Pa, USA.

  • Collis, C.M., M.J. Kim, H.W. Stokes, and R.M. Hall. 2002. Integron-encoded IntI integrases preferentially recognize the adjacent cognate attI site in recombination with a 59-be site. Mol. Microbiol. 46, 1415–1427.

    Article  PubMed  CAS  Google Scholar 

  • Danel, F., L.M.C. Hall, and D.M. Livermore. 1999. Laboratory mutants of OXA-10 β-lactamase giving ceftazidime resistance in Pseudomonas aeruginosa. J. Antimicrob. Chemother. 43, 339–344.

    Article  PubMed  CAS  Google Scholar 

  • Gu, B., M.Q. Tong, W.J. Zhao, G.Y. Liu, M.Z. Ning, S.Y. Pan, and W.S. Zhao. 2007. Prevalence and characterization of Class I integrons among Pseudomonas aeruginosa and Acinetobacter baumannii isolates from patients in Nanjing, China. J. Clin. Microbiol. 45, 241–243.

    Article  PubMed  CAS  Google Scholar 

  • Guerra, B., S.M. Soto, J.M. Argüelles, and M. Carmen. 2001. Multi-drug resistance is mediated by large plasmids carrying a class 1 integron in the emergent Salmonella enterica serotype [4,5,12:i:2]. Antimicrob. Agents Chemother. 45, 1305–1308.

    Article  PubMed  CAS  Google Scholar 

  • Kadlec, K., C. Kehrenberg, and S. Schwarz. 2007. Efflux-mediated resistance to florfenicol and/or chloramphenicol in Bordetella bronchiseptica: identification of a novel chloramphenicol exporter. J. Antimicrob. Chemother. 59, 191–196.

    Article  PubMed  CAS  Google Scholar 

  • Kehrenberg, C., B. Catry, F. Haesebrouck, A. Kruif, and S. Schwarz. 2005. Novel spectinomycin/streptomycin resistance gene, aadA14, from Pasteurella multocida. Antimicrob. Agents Chemother. 49, 3046–3049.

    Article  PubMed  CAS  Google Scholar 

  • Lee, K., J.H. Yum, D. Yong, H.M. Lee, H.D. Kim, J.D. Docquier, G.M. Rossolini, and Y. Chong. 2005. Novel acquired metallo-β-lactamase gene, blaSIM-1, in a class 1 integron from Acinetobacter baumannii clinical isolates from Korea. Antimicrob. Agents Chemother. 49, 4485–4491.

    Article  PubMed  CAS  Google Scholar 

  • Lévesque, C., L. Piché, C. Larose, and P.H. Roy. 1995. PCR mapping of integrons reveals several novel combinations of resistance genes. Antimicrob. Agents Chemother. 39, 185–191.

    PubMed  Google Scholar 

  • Lolans, K., A.M. Queenan, K. Bush, A. Sahud, and J.P. Quinn. 2005. First nosocomial outbreak of Pseudomonas aeruginosa producing an integron-borne metallo β-lactamase (VIM-2) in the United States. Antimicrob. Agents Chemother. 49, 3538–3540.

    Article  PubMed  CAS  Google Scholar 

  • Partridge, S.R., G.D. Recchia, C. Scaramuzzi, C.M. Collis, H.W. Stokes, and R.M. Hall. 2000. Definition of the attI1 site of class 1 integrons. Microbiology 146, 2855–2864.

    PubMed  CAS  Google Scholar 

  • Poirel, L., L. Cabanne, H. Vahaboglu, and P. Nordmann. 2005. Genetic enviroment and expression of the extended-spectrum β-lactamase bla PER-1 gene in Gram-negative bacteria. Antimicrob. Agents Chemother. 49, 1708–1713.

    Article  PubMed  CAS  Google Scholar 

  • Poirel, L., T. Lambert, S. Türköglu, E. Ronco, J.L. Gaillard, and P. Nordmann. 2001a. Characterization of class 1 integrons from Pseudomonas aeruginosa that contain the bla VIM-2 carbapenem-hydrolyzing β-lactamase gene and of two novel aminoglycoside resistance gene cassettes. Antimicrob. Agents Chemother. 45, 546–552.

    Article  PubMed  CAS  Google Scholar 

  • Poirel, L., V.O. Rotimi, E.M. Mokaddas, A. Karim, and P. Nordmann. 2001b. VEB-1-like extended-spectrum β-lactamases in Pseudomonas aeruginosa, Kuwait. Emerg. Infect. Dis. 7, 468–470.

    PubMed  CAS  Google Scholar 

  • Quale, J., S. Bratu, J. Gupta, and D. Landman. 2006. Interplay of efflux system, ampC, and oprD expression in carbapenem resistance of Pseudomonas aeruginosa clinical isolates. Antimicrob. Agents Chemother. 50, 1633–1641.

    Article  PubMed  CAS  Google Scholar 

  • Shibata, N., Y.D.K. Yamane, T. Yagi, H. Kurokawa, K. Shibayama, H. Kato, K. Kai, and Y. Arakawa. 2003. PCR typing of genetic determinants for metallo-β-lactamases and integrases carried by gram-negative bacteria isolated in Japan, with focus on the class 3 integron. J. Clin. Microbiol. 41, 5407–5413.

    Article  PubMed  CAS  Google Scholar 

  • Shmara, A., N. Weinsetel, K.J. Dery, R. Chavideh, and M.E. Tolmasky. 2001. Systematic analysis of a conserved region of the aminoglycoside 6′-N-acetyltransferase type Ib. Antimicrob. Agents Chemother. 45, 3287–3292.

    Article  PubMed  CAS  Google Scholar 

  • Stokes, H.W. and R.M. Hall. 1989. A novel family of potentially mobile DNA elements encoding site-specific gene-integration functions: integrons. Mol. Microbiol. 3, 1669–1683.

    Article  PubMed  CAS  Google Scholar 

  • Tenover, F.C., R.D. Arbeit, R.V. Goering, P.A. Mickelsen, B.E. Murray, D.H. Persing, and B. Swaminathan. 1995. Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing. J. Clin. Microbiol. 33, 2233–2239.

    PubMed  CAS  Google Scholar 

  • White, P.A., C.J. McIver, Y.M. Deng, and W.D. Rawlinson. 2000. Characterisation of two new gene cassettes, aadA5 and dfrA17. FEMS Microbiol. Lett. 182, 265–269.

    Article  PubMed  CAS  Google Scholar 

  • Yu, Y.S., T.T. Qu, J.Y. Zhou, J. Wang, H.Y. Li, and T.R. Walsh. 2006. Integrons containing the VIM-2 metallo-β-lactamase gene among imipenem-resistant Pseudomonas aeruginosa strains from different Chinese hospitals. J. Clin. Microbiol. 44, 4242–4245.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. The Department of Infectious Diseases, the First Affiliated Hospital with Nanjing Medical University, Nanjing, Jiangsu Province, 210029, P. R. China

    Yuan Wu, Hui Li, Jun Li & Zu Hu Huang

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  1. Yuan Wu
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  2. Hui Li
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  3. Jun Li
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  4. Zu Hu Huang
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Correspondence to Zu Hu Huang.

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These authors contributed equally to this work.

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Wu, Y., Li, H., Li, J. et al. Detection of Pseudomonas aeruginosa carried a new array of gene cassettes within class 1 integron isolated from a teaching hospital in Nanjing, China. J Microbiol. 46, 687–691 (2008). https://doi.org/10.1007/s12275-008-0021-6

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  • DOI: https://doi.org/10.1007/s12275-008-0021-6

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