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Antiproliferative and Apoptosis Inducing Effects of Non-Polar Fractions from Lawsonia inermis L. in Cervical (HeLa) Cancer Cells

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Abstract

Two non-polar fractions viz. hexane (Hex-LI) and chloroform fraction (CHCl3-LI) of Lawsonia inermis were studied for their antiproliferative potential in various cancer cell lines viz. HeLa, MCF-7, A549 and C6 glioma cells. Both the fractions showed more than 60 % of growth inhibition in all the tested cell lines at highest tested concentration. In clonogenic assay, different concentrations of Hex-LI and CHCl3-LI decreased the number and size of colonies as compared to control in HeLa cells. The apoptotic effects as nuclear condensation, fragmentation were visualized with Hoechst-33342 staining of HeLa cells using confocal microscope. Both fractions induced apoptotic cell death in human cervical carcinoma (HeLa) cells as evident from flow cytometric analysis carried out using Annexin V-FITC and propidium iodide dyes. CHCl3-LI treated cells significantly induced apoptosis (25.43 %) in comparison to control. Results from Neutral Comet assay demonstrated that both fractions induced double stranded breaks (DSB’s) in HeLa cells. Our data indicated that Hex-LI and CHCl3-LI treated cells showed significant increase of 32.2 and 18.56 % reactive oxygen species (ROS) levels in DCFH-DA assay respectively. Further, experimental studies to decipher exact pathway via which these fractions induce cell death are in progress.

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References

  • Ahmed S, Rahman A, Alam A, Saleem M, Athar M, Sultana S (2000) Evaluation of the efficacy of Lawsonia alba in the alleviation of carbon tetrachloride induced oxidative stress. J Ethnopharmacol 69:157–164

    Article  CAS  PubMed  Google Scholar 

  • Akerele O (1988) Medicinal plants and primary health care: an agenda for action. Fitoterapia 59:355–363

    Google Scholar 

  • Allen RT, Hunter WJ 3rd, Agarwal DK (1997) Morphological and biochemical characterization and analysis of apoptosis. J Pharmacol Toxicol Methods 37:215–228

    Article  CAS  PubMed  Google Scholar 

  • Azam S, Hadi N, Khan NU, Hadi SM (2004) Prooxidant property of green tea polyphenols epicatechin and epigallocatechin-3-gallate: implications for anticancer properties. Toxicol in Vitro 18:555–561

    Article  CAS  PubMed  Google Scholar 

  • Basirian M, Manjula SN, Mruthunjaya K (2013) In vitro anti-clastogenic activity of different fractions of roots of Lawsonia inermis on chromosomal aberration of human lymphocytes against doxocirubicin and cyclophosphamide as clastogens. Int J Med Med Sci 46:1239–1244

    Google Scholar 

  • Bich DH, Chung DQ, Chuong BX et al (2004) The medicinal plants and animals in Vietnam. Science and Technology Publishing House, Hanoi

    Google Scholar 

  • Camero A (2002) Targeting the cell cycle for cancer therapy. Br J Cancer 87:129–133

    Article  Google Scholar 

  • Chirinos R, Rogez H, Campos D, Pedreschi R, Larondelle Y (2007) Optimization of extraction conditions of antioxidant phenolic compounds from mashua (Tropaeolum tuberosum Ruíz & Pavón) tubers. Sep Purif Technol 55:217–225

    Article  CAS  Google Scholar 

  • Circu ML, Aw TY (2010) Reactive oxygen species, cellular redox systems, and apoptosis. Free Radic Biol Med 48:749–762

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Collins A, Dusinska M, Franklin M, Somorovská M, Petrovská H, Duthie S, Fillion L, Panayiotidis M, Raslová K, Vaughan N (1997) Comet assay in human biomonitoring studies: reliability, validation, and applications. Environ Mol Mutagen 30:139–146

    Article  CAS  PubMed  Google Scholar 

  • Conklin KA (2004) Free radicals: the pros and cons of antioxidants. Cancer chemotherapy and antioxidants. J Nutr 134:3201–3204

    Google Scholar 

  • Dai J, Mumper RJ (2010) Plant phenolics: extraction, analysis and their antioxidant and anticancer properties. Molecules 15:7313–7352

    Article  CAS  PubMed  Google Scholar 

  • Dasgupta T, Rao AR, Yadava PK (2003) Modulatory effect of Henna leaf (Lawsonia inermis) on drug metabolising phase I and phase II enzymes, antioxidant enzymes, lipid peroxidation and chemically induced skin and forestomach papillomagenesis in mice. Mol Cell Biochem 245:11–22

    Article  CAS  PubMed  Google Scholar 

  • Deeb D, Gao X, Jiang H, Janic B, Arbab AS, Rojanasakul Y, Dulchavsky SA, Gautam SC (2010) Oleanane triterpenoid CDDO-Me inhibits growth and induces apoptosis in prostate cancer cells through a ROS-dependent mechanism. Biochem Pharmacol 79:350–360

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Dhiman A, Sharma K, Goyal J, Garg M, Sharma A (2012) Determination of Lawsone content in leaves of Lawsonia inermis Linn. Int J Pharm Pharm Sci 1:17–20

    Google Scholar 

  • Ding H, Han C, Guo D, Chin YW, Ding Y, Kinghorn AD, D’Ambrosio SM (2009) Selective induction of apoptosis of human oral cancer cell lines by avocado extracts via a ROS-mediated mechanism. Nutr Cancer 61:348–356

    Article  CAS  PubMed  Google Scholar 

  • Drees M, Dengler WA, Roth T, Labonte H, Mayo J, Malspeis L, Grever M, Sausville EA, Fiebig HH (1997) Flavopiridol (L86-8275): selective antitumor activity in vitro and activity in vivo for prostate carcinoma cells. Clin Cancer Res 3:273–279

    CAS  PubMed  Google Scholar 

  • Edderkaoui M, Odinokova I, Ohno I, Gukovsky I, Go VL, Pandol SJ, Gukovskaya AS (2008) Ellagic acid induces apoptosis through inhibition of nuclear factor kappa B in pancreatic cancer cells. World J Gastroenterol 14(23):3672–3680

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Elmore S (2007) Apoptosis: a review of programmed cell death. Toxicol Pathol 35:495–516

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Franken NAP, Rodermond HM, Stap J, Haveman J, Bree CV (2006) Clonogenic assay of cells in vitro. Nat Protoc 5:2315–2319

    Article  Google Scholar 

  • Guha G, Rajkumar V, Ashok Kumar R, Mathew L (2011) Antioxidant activity of Lawsonia inermis extracts inhibits chromium(vi)-induced cellular and DNA toxicity. Evid Based Complement Alternat Med. doi:10.1093/ecam/nep205

    Google Scholar 

  • Gundala SR, Yang C, Lakshminarayana N, Asif G, Gupta MV, Shamsi S, Aneja R (2013) Polar biophenolics in sweet potato greens extract synergize to inhibit prostate cancer cell proliferation and in vivo tumor growth. Carcinogenesis 34(9):2039–2049

    Article  CAS  PubMed  Google Scholar 

  • Gundala SR, Yang C, Mukkavilli R, Paranjpe R, Brahmbhatt M, Pannu V, Cheng A, Reid MD, Aneja R (2014) Hydroxychavicol, a betel leaf component, inhibits prostate cancer through ROS-driven DNA damage and apoptosis. Toxicol Appl Pharmacol 280:86–96

    Article  CAS  PubMed  Google Scholar 

  • Gurib-Fakim A (2006) Medicinal plants: traditions of yesterday and drugs of tomorrow. Mol Aspects Med 27:1–93

    Article  CAS  PubMed  Google Scholar 

  • Han DH, Lee MJ, Kim JH (2006) Antioxidant and apoptosis-inducing activities of ellagic acid. Anticancer Res 26(5A):3601–3606

    CAS  PubMed  Google Scholar 

  • Haneef J, Parvathy M, Thankayyan RSK, Sithul H, Sreeharshan S (2012) Bax translocation mediated mitochondrial apoptosis and caspase dependent photosensitizing effect of Ficus religiosa on cancer cells. PLoS ONE 7(7):e40055. doi:10.1371/journal.pone.0040055

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Ho CC, Huang AC, Yu CS, Lien JC, Wu SH, Huang YP, Huang HY, Kuo JH, Liao WY, Yang JS, Chen PY, Chung JG (2014) Ellagic acid induces apoptosis in tsgh8301 human bladder cancer cells through the endoplasmic reticulum stress- and mitochondria-dependent signaling pathways. Environ Toxicol 29:1262–1274

    CAS  PubMed  Google Scholar 

  • Hsieh TJ, Liu TZ, Lu FJ, Hsieh PY, Chen CH (2006) Actinodaphnine induces apoptosis through increased nitric oxide, reactive oxygen species and down regulation of NF-kappa B signaling in human hepatoma Mahlavu cells. Food Chem Toxicol 44:344–354

    Article  CAS  PubMed  Google Scholar 

  • Hsouna AB, Trigui M, Culioli G, Blache Y, Jaoua S (2011) Antioxidant constituents from Lawsonia inermis leaves: isolation, structure elucidation and antioxidative capacity. Food Chem 125:193–200

    Article  Google Scholar 

  • Hsu H-F, Huang K-H, Lu K-J, Chiou S-J, Yen J-H, Chang C-C, Houng J-Y (2011) Typhonium blumei extract inhibits proliferation of human lung adenocarcinoma A549 cells via induction of cell cycle arrest and apoptosis. J Ethnopharmacol 135(2):492–500

    Article  PubMed  Google Scholar 

  • Hu W, Kavanagh JJ (2003) Anticancer therapy targeting the apoptotic pathway. Lancet Oncol 4:721–729

    Article  CAS  PubMed  Google Scholar 

  • Huang WW, Chiu YJ, Fan MJ, Lu HF, Yeh HF, Li KH, Chen PY, Chung JG, Yang JS (2010) Kaempferol induced apoptosis via endoplasmic reticulum stress and mitochondria-dependent pathway in human osteosarcoma U-2 OS cells. Mol Nutr Food Res 54(11):1585–1595

    Article  CAS  PubMed  Google Scholar 

  • Igney F, Krammer P (2002) Death and anti-death: tumour resistance to apoptosis. Nat Rev Cancer 2:277–288

    Article  CAS  PubMed  Google Scholar 

  • Jeong JC, Jang SW, Kim TH, Kwon CH, Kim YK (2010) Mulberry fruit (Moris fruc-tus) extracts induce human glioma cell death in vitro through ROS-dependent mitochondrial pathway and inhibits glioma tumor growth in vivo. Nutr Cancer 62:402–412

    Article  PubMed  Google Scholar 

  • Jiny Varghese K, Silvipriya KS, Resmi S, Jolly CI (2010) Lawsonia Inermis (Henna): a natural dye of various therapeutic uses - a review. Inventi Rapid: Cosmeceuticals 1:1–5

    Google Scholar 

  • Jung IL (2014) Soluble extract from Moringa oleifera leaves with a new anticancer activity. PLoS ONE 9(4):e95492. doi:10.1371/journal.pone.0095492

    Article  PubMed Central  PubMed  Google Scholar 

  • Karna P, Gundala SR, Gupta MV, Shamsi SA, Pace RD, Yates C, Narayan S, Aneja R (2011) Polyphenol-rich sweet potato greens extract inhibits proliferation and induces apoptosis in prostate cancer cells in vitro and in vivo. Carcinogenesis 32(12):1872–1880

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Kelloff GJ, Crowell JA, Steele VE, Lubet RA, Malone WA, Boone CW, Kopelovich L, Hawk ET, Lieberman R, Lawrence JA, Ali I, Viner JL, Sigman CC (2000) Progress in cancer chemoprevention: development of diet-derived chemopreventive agents. J Nutr 130(2S):467S–471S

    CAS  PubMed  Google Scholar 

  • Kerr JF, Wyllie AH, Currie AR (1972) Apoptosis: a basic biological phenomenon with wide-ranging implications in tissue kinetics. Br J Cancer 26:239–257

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Kim EJ, Lim SS, Park SY, Shin HK, Kim JS, Park JHY (2008) Apoptosis of DU145 human prostate cancer cells induced by dehydrocostus lactone isolated from the root of Saussurea lappa. Food Chem Toxicol 46(12):3651–3658

    Article  CAS  PubMed  Google Scholar 

  • Kim HJ, Han MH, Kim GY, Choi YW, Choi YH (2012) Hexane extracts of garlic cloves induce apoptosis through the generation of reactive oxygen species in Hep3B human hepatocarcinoma cells. Oncol Rep 28:1757–1763

    PubMed  Google Scholar 

  • Krishnaiah D, Sarbathy R, Nithyanandam R (2011) A review of the antioxidant potential of medicinal plant species. Food Bioprod Process 89:217–233

    Article  CAS  Google Scholar 

  • Kroemer G, Reed JC (2000) Mitochondrial control of cell death. Nat Med 6(5):513–519

    Article  CAS  PubMed  Google Scholar 

  • Kroemer G, Petit PX, Zamzami N, Vayssiere JL, Mignotte B (1995) The biochemistry of programmed cell death. FASEB J 9:1277–1287

    CAS  PubMed  Google Scholar 

  • Kumar M, Kumar S, Kaur SJ (2014) Identification of polyphenols in leaf extracts of Lawsonia inermis L. with antioxidant, antigenotoxic and antiproliferative potential. Int J Green Pharm 8:23–36

    Article  Google Scholar 

  • Kutluk T, Kars A (1998) General knowledge about cancer. Ankara; Turkey cancer investigation and fight society publications, pp: 7–15

  • Lafka TI, Sinanoglou VJ, Lazos ES (2007) On the extraction and antioxidant activity of phenolic compounds from winery wastes. Food Chem 104:1206–1214

    Article  CAS  Google Scholar 

  • Lafka TI, Lazou AE, Sinanoglou VJ, Lazos ES (2013) Phenolic extracts from wild olive leaves and their potential as edible oils antioxidants. Foods 2:18–31

    Article  Google Scholar 

  • Lam M, Carmichael AR, Griffiths HR (2012) An aqueous extract of Fagonia cretica induces DNA damage, cell cycle arrest and apoptosis in breast cancer cells via FOXO3a and p53 expression. PLoS ONE 7(6):e40152. doi:10.1371/journal.pone.0040152

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Lee HZ, Liu WZ, Hsieh WT, Tang FY, Chung JG, Leung HWC (2009) Oxidative stress involvement in Physalis angulata -induced apoptosis in human oral cancer cells. Food Chem Toxicol 47:561–570

    Article  CAS  PubMed  Google Scholar 

  • Li XK, Motwani M, Tong W, Bornmann W, Schwartz GK, Huanglian A (2000) Chinese herbal extract, inhibits cell growth by suppressing the expression of cyclin B1 and inhibiting CDC2 kinase activity in human cancer cells. Mol Pharm 58:1287–1293

    CAS  Google Scholar 

  • Lim SW, Ting KN, Bradshaw TD, Zeenathul NA, Wiart C, Khoo TJ, Lim KH, Loh HS (2011) Acalypha wilkesiana extracts induce apoptosis by causing single strand and double strand DNA breaks. J Ethnopharmacol 138(2):616–623

    Article  CAS  PubMed  Google Scholar 

  • Lizard G, Fournel S, Genestier L (1995) Kinetics of plasma membrane and mitochondrial alterations in cells undergoing apoptosis. Cytometry 21:275–283

    Article  CAS  PubMed  Google Scholar 

  • Machana S, Weerapreeyakul N, Barusrux S, Thumanu K, Tanthanuch W (2012) Synergistic anticancer effect of the extracts from Polyalthia evecta caused apoptosis in human hepatoma (HepG2) cells. Asian Pac J Trop Biomed 2:589–596

    Article  PubMed Central  PubMed  Google Scholar 

  • Matkar SS, Wrischnik LA, Jones PR, Hellmann-Blumberg U (2006) Two closely related nickel complexes have different effects on DNA damage and cell viability. Biochem Biophys Res Commun 343:754–761

    Article  CAS  PubMed  Google Scholar 

  • Mickisch G, Fajta S, Keilhauer G, Schlike E, Tschada P, Alken P (1990) Chemosensitivity testing of primary human renal cell carcinoma by tetrazolium based microculture assay (MTT). Urol Res 18:131–136

    Article  CAS  PubMed  Google Scholar 

  • Militao GCG, Dantas INF, Pessoa C, Falcão MJC, Silveira ER, Lima MAS, Curi R, Lima T, Moraes MO, Costa-Lotufo LV (2006) Induction of apoptosis by pterocarpans from Platymiscium floribundum in HL-60 human leukemia cells. Life Sci 78:2409–2417

    Article  CAS  PubMed  Google Scholar 

  • Millan A, Huerta MD (2009) Apoptosis-inducing factor and colon cancer. J Surg Res 151:163–170

    Article  CAS  PubMed  Google Scholar 

  • Moure A, Cruz JM, Franco D, Dominguez JM, Sineiro J, Dominguez H, Núñez MJ, Parajó JC (2001) Natural antioxidants from residual sources. Food Chem 2:145–171

    Article  Google Scholar 

  • Munshi A, Hobbs M, Meyn RE (2005) Clonogenic cell survival assay . From: Methods in Molecular Medicine, vol 110:Chemosensitivity vol. 1. In: Blumenthal RD (ed) In vitro Assays. Humana Press Inc, Totowa, NJ

  • Owoabi J, Omogbai EKI, Obasuyi O (2007) Antifungal and antibacterial activities of the ethanolic and aqueous extract of Kigella africana (Bignoniaceae) stem bark. Afr J Biotechnol 6:882–885

    Google Scholar 

  • Pacifico S, Gallicchio M, Lorenz P, Potenza N, Galasso S, Marciano S, Fiorentino A, Stintzing FC, Monaco P (2013) Apolar Laurus nobilis leaf extracts induce cytotoxicity and apoptosis towards three nervous system cell lines. Food Chem Toxicol 62:628–637

    Article  CAS  PubMed  Google Scholar 

  • Pratumvinit B, Srisapoomi T, Worawattananon P, Opartkiattikul N, Jiratchariyakul W, Kummalue T (2009) In vitro antineoplastic effect of Ficus hispida L. plant against breast cancer cell lines. J Med Plant Res 3(4):255–261

    Google Scholar 

  • Priya R, Ilavenil S, Kaleeswaran B, Srigopalram S, Ravikumar S (2011) Effect of Lawsonia inermis on tumor expression induced by Dalton’s lymphoma ascites in Swiss albino mice. Saudi J Biol Sci 18:353–359

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Qi F, Li A, Zhao L, Xu H, Inagaki Y, Wang D, Cui X, Gao B, Kokudo N, Nakata M, Tang W (2010) Cinobufacini, an aqueous extract from Bufo bufo gargarizans Cantor, induces apoptosis through a mitochondria mediated pathway in human hepatocellular carcinoma cells. J Ethnopharmacol 128:654–661

    Article  PubMed  Google Scholar 

  • Raja W, Agrawal RC, Ovais M (2008) Evaluation of antimutagenocity effect of Lawsonia inermis (henna) leaf extract in Swiss albino mice. Res J Pharm Technol 1(3):278–279

    Google Scholar 

  • Ramasamy S, Wahabb NA, Abidina NZ, Manickam S (2013) Effect of extracts from Phyllanthus watsonii Airy Shaw on cell apoptosis in cultured human breast cancer MCF-7 cells. Exp Toxicol Pathol 65:341–349

    Article  CAS  PubMed  Google Scholar 

  • Rao PS, Ramanadham M, Prasad MNV (2009) Anti-proliferative and cytotoxic effects of Strychnos nux-vomica root extract on human multiple myeloma cell line – RPMI 8226. Food Chem Toxicol 47:283–288

    Article  CAS  PubMed  Google Scholar 

  • Reddy KR (1988) Folk medicine from Chittoor district Andhra Pradesh, India used in the treatment of jaundice. Int J Crude Drug Res 26:137–140

    Google Scholar 

  • Reed JC (2003) Apoptosis-targeted therapies for cancer. Cancer Cell 3:17

    Article  CAS  PubMed  Google Scholar 

  • Sadeghi-aliabadi H, Ghasemi N, Kohi M (2008) Cytotoxic effect of Convolvulus arvensis extracts on human cancerous cell line. Res Pharm Sci 3:31–34

    Google Scholar 

  • Schumacker PT (2006) Reactive oxygen species in cancer cells: live by the sword, die by the sword. Cancer Cell 10:175–176

    Article  CAS  PubMed  Google Scholar 

  • Sharma V, Joseph C, Ghosh S, Agarwal A, Mishra MK, Sen E (2007) Kaempferol induces apoptosis in glioblastoma cells through oxidative stress. Mol Cancer Ther 6(9):2544–2553

    Article  CAS  PubMed  Google Scholar 

  • Simon HU, Haj-Yehia A, Levi-Schaffer F (2000) Role of reactive oxygen species in apoptosis induction. Apoptosis 5:415–418

    Article  CAS  PubMed  Google Scholar 

  • Szatrowski TP, Nathan CF (1991) Production of large amounts of hydrogen peroxide by human tumor cells. Cancer Res 51:794–798

    CAS  PubMed  Google Scholar 

  • Taraphdar AK, Roy M, Bhattacharya RK (2001) Natural products as inducers of apoptosis: implication for cancer therapy and prevention. Curr Sci 80:1387–1396

    CAS  Google Scholar 

  • Thayyullathil F, Chathoth S, Hago A, Patel M, Galadari S (2008) Rapid reactive oxygen species (ROS) generation induced by curcumin leads to caspase-dependent and -independent apoptosis in L929 cells. Free Radic Biol Med 45:1403–1412

    Article  CAS  PubMed  Google Scholar 

  • Trachootham D, Alexandre J, Huang P (2009) Targeting cancer cells by ROS-mediated mechanisms: a radical therapeutic approach? Nat Rev Drug Discov 8:579–591

    Article  CAS  PubMed  Google Scholar 

  • Vasanthi P, Ganapathy M, Evanjelene VK, Ayyavuv N, Angamuthu J (2014) Phytochemical screening and antioxidant activity of extracts of the leaf and bark of Albizzia lebbeck (Benth). Acad J Med Plant 2:026–031

    CAS  Google Scholar 

  • Vermes I, Haanen C, Steffens-Nakken H, Reutelingsperger C (1995) A novel assay for apoptosis, flow cytometric detection of phosphatidylserin expression on early apoptotic cells using fluorescein labeled annexin-V. J Immunol Methods 184:39–51

    Article  CAS  PubMed  Google Scholar 

  • Vickers A (2002) Botanical medicines for the treatment of cancer: rationale, overview of current data, and methodological considerations for phase I and II trials. Cancer Investig 20:1069–1079

    Article  Google Scholar 

  • Wang ZB, Liu YQ, Cui YF (2005) Pathways to caspase activation. Cell Biol Int 29:489–496

    Article  CAS  PubMed  Google Scholar 

  • Wang CZ, Calway T, Yuan CS (2012) Herbal medicines as adjuvants for cancer therapeutics. Am J Chin Med 40:657–669

    Article  PubMed Central  PubMed  Google Scholar 

  • Wang CZ, Zhang Z, Huang WH, Du GJ, Wen XD, Calway T, Yu C, Nass R, Zhao J, Du W, Li SP, Yuan CS (2013) Identification of potential anticancer compounds from Oplopanax horridus. Phytomedicine 20(11):999–1006

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Warrier PK (2004) Indian medicinal plants-a compendium of 500 species. Orient longman private limited, Chennai, pp 303–304

  • Widodo N, Priyandoko D, Shah N, Wadhwa R, Kaul SC (2010) Selective killing of cancer cells by Ashwagandha leaf extract and its component Withanone involves ROS signaling. PLoS ONE 5:e13536

    Article  PubMed Central  PubMed  Google Scholar 

  • Wojewodzka M, Buraczewska I, Kruszewski M (2002) A modified neutral comet assay: elimination of lysis at high temperature and validation of the assay with anti-single-stranded DNA antibody. Mutat Res 518:9–20

    Article  CAS  PubMed  Google Scholar 

  • Wu SJ, Chang SP, Lin DL, Wang SS, Hou FF, Ng LT (2009) Supercritical carbon dioxide extract of Physalis peruviana induced cell cycle arrest and apoptosis in human lung cancer H661 cells. Food Chem Toxicol 47:1132–1138

    Article  CAS  PubMed  Google Scholar 

  • Wyllie AH, Kerr JF, Currie AR (1980) Cell death: the significance of apoptosis. Int Rev Cytol 68:251–306

    Article  CAS  PubMed  Google Scholar 

  • Xie F, Su M, Qiu W, Zhang M, Guo Z, Su B, Liu J, Li X, Zhou L (2013) Kaempferol promotes apoptosis in human bladder cancer cells by inducing the tumor suppressor, PTEN. Int J Mol Sci 14(11):21215–21226

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Xu Y, Ge R, Du J, Xin H, Yi T, Sheng J, Wang Y, Ling C (2009) Corosolic acid induces apoptosis through mitochondrial pathway and caspases activation in human cervix adenocarcinoma HeLa cells. Cancer Lett 284:229–237

    Article  CAS  PubMed  Google Scholar 

  • Zhang J, Xu M (2002) Apoptotic DNA fragmentation and tissue homeostasis. Trends Cell Biol 12(2):84–89

    Article  CAS  PubMed  Google Scholar 

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Acknowledgments

This work was supported by the Council of Scientific and Industrial Research (CSIR) [38(1265)/10/EMR-II], New Delhi (India) and the UPE programme of University Grants Commission (UGC), New Delhi.

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  1. Department of Botanical and Environmental Sciences, Guru Nanak Dev University, Amritsar, 143005, Punjab, India

    Manish Kumar, Paramjeet Kaur & Satwinderjeet Kaur

  2. Department of Chemistry, UGC Centre for Advanced Studies, Guru Nanak Dev University, Amritsar, 143005, Punjab, India

    Subodh Kumar

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Kumar, M., Kaur, P., Kumar, S. et al. Antiproliferative and Apoptosis Inducing Effects of Non-Polar Fractions from Lawsonia inermis L. in Cervical (HeLa) Cancer Cells. Physiol Mol Biol Plants 21, 249–260 (2015). https://doi.org/10.1007/s12298-015-0285-3

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