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Probiotics Supplemented with Omega-3 Fatty Acids are More Effective for Hepatic Steatosis Reduction in an Animal Model of Obesity

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Abstract

Today probiotics have been suggested as a treatment for the prevention of NAFLD. Omega-3 fatty acid supplementation may have beneficial effects in regulating hepatic lipid metabolism, adipose tissue function and inflammation. The present study was designed to determine whether probiotics plus omega-3 are superior to probiotics alone on the monosodium glutamate (MSG)-induced NAFLD model in rats. We included 60 rats divided into four groups, 15 animals in each. Rats of group I were intact. Newborn rats of groups II–IV were injected with MSG. The III (Symbiter) group received 2.5 ml/kg of multiprobiotic “Symbiter” containing concentrated biomass of 14 probiotic bacteria genera. The IV (Symbiter-Omega) groups received “Symbiter-Omega” combination of probiotic biomass supplemented with flax and wheat germ oil (250 mg of each, concentration of omega-3 fatty acids 1–5 %). In both interventional groups reduction in total NAS score was observed. Supplementation of alive probiotic mixture with omega-3 fatty acids lead to 20 % higher decrease in steatosis score (0.73 ± 0.11 vs 0.93 ± 0.22, p = 0.848) and reduction by 16.6 % of triglycerides content in liver as compared to probiotic alone. Our study demonstrated more pronounced reduction in hepatic steatosis and hepatic lipid accumulation after treatment with combination of alive probiotics and omega-3 as compared to probiotics alone.

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Acknowledgments

The authors express their sincere thanks to Dr. Yankovsky Dmitro Stanislavovych for the help, advice and financial support of this work.

Authors’ Contribution

Petro Bodnar and Tetyana Beregova conceived and designed the study. Nazarii Kobyliak and Tetyana Falalyeyeva carried out experiments, collected data, analyzed data and wrote the manuscript. All authors have read and approved the final version to be published.

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Authors and Affiliations

  1. Bogomolets National Medical University, T. Shevchenko Boulevard, 13, Kiev, 01601, Ukraine

    Nazarii Kobyliak & Petro Bodnar

  2. Taras Shevchenko National University of Kyiv, Volodymyrska Str., 64/13, Kiev, 01601, Ukraine

    Tetyana Falalyeyeva & Tetyana Beregova

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  1. Nazarii Kobyliak
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Correspondence to Nazarii Kobyliak.

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Ethical Approval

This study was carried out in strict accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health and the general ethical principles of animal experiments, approved by the First National Congress on Bioethics Ukraine (September 2001). The protocol was approved by the Committee on the Ethics of Animal Experiments of the Taras Shevchenko National University of Kyiv (Protocol Number: 17/2015).

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Kobyliak, N., Falalyeyeva, T., Bodnar, P. et al. Probiotics Supplemented with Omega-3 Fatty Acids are More Effective for Hepatic Steatosis Reduction in an Animal Model of Obesity. Probiotics & Antimicro. Prot. 9, 123–130 (2017). https://doi.org/10.1007/s12602-016-9230-1

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