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Influence of secreted factors from human adipose tissue on glucose utilization and proinflammatory reaction

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Abstract

The objective of the present study was to characterize the nature of the autocrine/paracrine signal within human adipose tissue that may alter glucose metabolism and the inflammatory status in adipocytes. We prepared a conditioned medium from abdominal dermolipectomies in the absence (CM) or the presence (CMBSA) of bovine serum albumin (BSA), and we tested the influence of CM and CMBSA on glucose transport, maximal insulin response, and the expression of inflammation marker genes in differentiated human SGBS adipocytes. We found that CMBSA increased basal and reduced insulin-stimulated glucose incorporation along with a reduced mRNA level of the glucose transport GLUT4, and an increased expression of GLUT1. These effects were associated with a potent upregulation in the mRNA level of the proinflammatory cytokines IL-6 and MCP-1. These regulations were strongly attenuated in the absence of BSA during the preparation of CM, or after BSA depletion of CM, and were attributed to water-soluble molecules rather than lipids. Finally, fractionation of CMBSA by isoelectric focusing showed that part of its bioactivity could be reproduced with proteins with pHi ranging from 6.6 to 7.6. In conclusion, our results demonstrate that the production by human adipose tissue of autocrine/paracrine neutral proteins is able to increase the inflammatory status of the adipocytes and to deteriorate their glucose metabolism and maximal insulin response, and their release is greatly amplified by the presence of albumin.

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Acknowledgments

This work was supported by grants from INSERM and the “Fondation pour la Recherche Médicale” (grant #DRM20101220459).

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Authors and Affiliations

  1. Institut National de la Santé et de la Recherche Médicale (INSERM), U1048, Toulouse, France

    Karine Tréguer, Rodolphe Dusaulcy, Sandra Grès, Estelle Wanecq, Philippe Valet & Jean Sébastien Saulnier-Blache

  2. Institut des Maladies Métaboliques et Cardiovasculaires (I2MC), Université Paul Sabatier, Université de Toulouse, Toulouse, France

    Karine Tréguer, Rodolphe Dusaulcy, Sandra Grès, Estelle Wanecq, Philippe Valet & Jean Sébastien Saulnier-Blache

  3. I2MR, IFR 31, BP 84225, 31432, Toulouse cedex 4, France

    Jean Sébastien Saulnier-Blache

Authors
  1. Karine Tréguer
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  2. Rodolphe Dusaulcy
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  3. Sandra Grès
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  4. Estelle Wanecq
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  5. Philippe Valet
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  6. Jean Sébastien Saulnier-Blache
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Correspondence to Jean Sébastien Saulnier-Blache.

Additional information

Karine Tréguer and Rodolphe Dusaulcy contributed equally to this work.

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Tréguer, K., Dusaulcy, R., Grès, S. et al. Influence of secreted factors from human adipose tissue on glucose utilization and proinflammatory reaction. J Physiol Biochem 69, 625–632 (2013). https://doi.org/10.1007/s13105-013-0238-7

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  • DOI: https://doi.org/10.1007/s13105-013-0238-7

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