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Protective effect of rosmarinic acid against oxidative stress biomarkers in liver and kidney of strepotozotocin-induced diabetic rats

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Abstract

In the present study, we investigated the efficiency of rosmarinic acid (RA) in preventing the alteration of oxidative parameters in the liver and kidney of diabetic rats induced by streptozotocin (STZ). The animals were divided into six groups (n = 8): control, ethanol, RA 10 mg/kg, diabetic, diabetic/ethanol, and diabetic/RA 10 mg/kg. After 3 weeks of treatment, we found that TBARS levels in liver and kidney were significantly increased in the diabetic/saline group and the administration of RA prevented this increase in the liver and kidney (P < 0.05). Diabetes caused a significant decrease in the activity of superoxide dismutase (SOD) and catalase (CAT) in the diabetes/saline group (P < 0.05). However, the treatment with 10 mg/kg RA (antioxidant) prevented this alteration in SOD and CAT activity in the diabetic RA group (P < 0.05). In addition, RA reverses the decrease in ascorbic acid and non-protein-thiol (NPSH) levels in diabetic rats. The treatment with RA also prevented the decrease in the Delta-aminolevulinic acid dehydratase (ALA-D) activity in the liver and kidney of diabetic rats. Furthermore, RA did not have any effect on glycemic levels. These results indicate that RA effectively reduced the oxidative stress induced by STZ, suggesting that RA is a potential candidate for the prevention and treatment of pathological conditions in diabetic models.

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Acknowledgments

We wish to thank the Academy of Sciences for the Developing World (TWAS) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) for the financial support.

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Authors and Affiliations

  1. Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Campus Universitário, Camobi, 97105-900, Santa Maria, RS, Brazil

    Nadia Mushtaq, Roberta Schmatz, Luciane Belmonte Pereira, Pauline da Costa, Karine Paula Reichert, Diéssica Dalenogare, Luana Paula Pelinson, Juliano Marchi Vieira, Naiara Stefanello, Lizielle Souza de Oliveira, Vera Maria Morsch & Maria Rosa Schetinger

  2. Department of Biotechnology, University of Science and Technology, Bannu, Khyber Pakhtunkhwa, Pakistan

    Mushtaq Ahmed

  3. Department of NEUROFARBA, University of Florence, Via Ugo Schiff 6, Sesto F.no (Firenze), 50019, Italy

    Nadia Mulinacci & Maria Bellumori

  4. Instituto Federal de Educação, Ciência e Tecnologia do Rio Grande do Sul, IFRS-Câmpus Ibirubá, 98200-000, Ibirubá, Brazil

    Roberta Schmatz

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  1. Nadia Mushtaq
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Correspondence to Roberta Schmatz or Maria Rosa Schetinger.

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Mushtaq, N., Schmatz, R., Ahmed, M. et al. Protective effect of rosmarinic acid against oxidative stress biomarkers in liver and kidney of strepotozotocin-induced diabetic rats. J Physiol Biochem 71, 743–751 (2015). https://doi.org/10.1007/s13105-015-0438-4

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  • DOI: https://doi.org/10.1007/s13105-015-0438-4

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