Skip to main content
SpringerLink
Log in

Evaluation du test de diagnostic rapide du paludisme OptiMal-IT® pLDH à la limite de la distribution de Plasmodium falciparum en Mauritanie

Evaluation of malaria rapid diagnostic test Optimal-IT® pLDH along the Plasmodium falciparum distribution limit in Mauritania

  • Biologie Clinique / Clinical Biology
  • Published:
Bulletin de la Société de pathologie exotique

Résumé

Les performances du test de diagnostic rapide (TDR) du paludisme OptiMal-IT® ont été évaluées en Mauritanie où la transmission du paludisme est faible, de type saisonnière et courte. La microscopie a été prise comme méthode de diagnostique de référence. L’inclusion des patients fébriles venus en consultation dans six structures sanitaires, dont trois en milieu urbain et trois en milieu rural, s’est déroulée de décembre 2011 à février 2012 et d’août 2012 à mars 2013. Au total, 780 patients ont été inclus et 759 avaient à la fois un résultat de TDR et de goutte épaisse. La lecture des 774 lames, dont 200 étaient positives, a décelé P. falciparum ou P. vivax dans les proportions respectives de 63,5 % (127/200) et 29,5 % (59/200), et la co-infection P. falciparum/P. vivax a représenté 7 % (14/200). Les deux espèces ont été observées dans les divers sites avec des proportions significativement différentes. Les proportions de lames porteuses d’hématozoaires du paludisme et de TDR positifs étaient respectivement de 26,3 % et 30,3 %. La sensibilité et la spécificité du TDR étaient respectivement de 89 % [84,7-93,3] et 91,1 % [88,6-93,4]. Les valeurs prédictives positives et négatives étaient de 78,1 % [72,2-83,7] et 95,9 % [94,1-97,5]. Les valeurs diagnostiques trouvées étaient comparables à celles généralement rapportées. Les TDR tendent à devenir le principal outil de diagnostic du paludisme en Mauritanie.

Abstract

Performance of the malaria Rapid Diagnostic Test (RDT) OptiMal-IT® was evaluated in Mauritania where malaria is low and dependent on a short transmission season. Slide microscopy was considered as the reference method of diagnosis. Febrile patients with suspected malaria were recruited from six health facilities, 3 urban and 3 rural, during two periods (December 2011 to February 2012, and August 2012 to March 2013). Overall, 780 patients were sampled, with RDT and thick blood film microscopy results being obtained for 759 of them. Out of 774 slides examined, of which 200 were positive, P. falciparum and P. vivax mono-infections were detected in 63.5% (127) and 29.5% (59), while P. falciparum/P. vivax coinfections were detected in 7% (14). Both species were observed in all study sites, although in significantly different proportions. The proportions of thick blood film and OptiMal-IT® RDT positive individuals was 26.3% and 30.3% respectively. Sensitivity and specificity of OptiMal-IT® RDT were 89% [95% CI, 84.7-93.3] and 91.1% [88.6-93.4]. Positives and negative predictive values were 78.1% [72.2-83.7] and 95.9% [94.1-97.5]. These diagnostic values are similar to those generally reported elsewhere, and support the use of RDTs as the main diagnostic tool for malaria in Mauritanian health facilities. In the future, choice of RDTs to be used must take account of thermostability in a hot, dry environment and their ability to detect P. falciparum and P. vivax.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Références

  1. Abba K, Deeks JJ, Olliaro P, et al (2011) Rapid diagnostic tests for diagnosing uncomplicated P. falciparum malaria in endemic countries. Cochrane Database Syst Rev (7):CD008122

  2. Ashley EA, Touabi M, Ahrer M, et al (2009) Evaluation of three parasite lactate dehydrogenase-based rapid diagnostic tests for the diagnosis of falciparum and vivax malaria. Malar J 8:241

    Article  PubMed  PubMed Central  Google Scholar 

  3. Ba H, Duffy CW, Ahouidi AD, et al (2016) Widespread distribution of Plasmodium vivax malaria in Mauritania on the interface of the Maghreb and West Africa. Malar J 15:80

    Article  PubMed  PubMed Central  Google Scholar 

  4. Ceesay SJ, Casals-Pascual C, Erskine J, et al (2008) Changes in malaria indices between 1999 and 2007 in The Gambia: a retrospective analysis. Lancet 372(9649):1545–54

    Article  PubMed  PubMed Central  Google Scholar 

  5. Chiodini PL, Bowers K, Jorgensen P, et al (2007) The heat stability of Plasmodium lactate dehydrogenase-based and histidinerich protein 2-based malaria rapid diagnostic tests. Trans R Soc Trop Med Hyg 101(4):331–7

    Article  CAS  PubMed  Google Scholar 

  6. Dia I, Ba H, Mohamed SA, et al (2009) Distribution, host preference and infection rates of malaria vectors in Mauritania. Parasit Vectors 2(1):61

    Article  PubMed  PubMed Central  Google Scholar 

  7. Diarra A, Nébié I, Tiono A, et al (2012) Seasonal performance of a malaria rapid diagnosis test at community health clinics in a malaria-hyperendemic region of Burkina Faso. Parasit Vectors 5:103

    Article  PubMed  PubMed Central  Google Scholar 

  8. Hernandez E, De Pina JJ, Fabre R, et al (2001) Evaluation du test OptiMal® dans le diagnostic des accès palustres d’importation. Méd Trop 61(2):153–57

    CAS  Google Scholar 

  9. Heutmekers M, Gillet P, Maltha J, et al (2012) Evaluation of the rapid diagnostic test Care Start pLDH malaria (pf-pldh/pan-pldh) for the diagnosis of malaria in a reference setting. Malar J 11:204

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Kahama-Maro J, D’Acremont V, Mtasiwa D, et al (2011) Low quality of routine microscopy for malaria at different levels of the health system in Dar es Salaam. Malar J 10:332

    Article  PubMed  PubMed Central  Google Scholar 

  11. Kakkilaya BS (2003) Rapid diagnosis of malaria. Laboratory Medicine 34(8):602–8

    Article  Google Scholar 

  12. Laferi H, Kandel K, Pichler H (1997) False Positive Dipstick Test for Malaria. N Engl J Med 337(22):1635–6

    Article  CAS  PubMed  Google Scholar 

  13. Lekweiry KM, Basco LK, Salem MS, et al (2011) Malaria prevalence and morbidity among children reporting at health facilities in Nouakchott, Mauritania. Trans R Soc Trop Med Hyg 105(12):727–33

    Article  PubMed  Google Scholar 

  14. Lekweiry KM, Ould Mohamed Salem Boukhary A, Gaillard T, et al (2012) Molecular surveillance of drug-resistant Plasmodium vivax using pvdhfr, pvdhps and pvmdr1 markers in Nouakchott, Mauritania. J Antimicrob Chemother 67(2):367–74

    Article  Google Scholar 

  15. Lekweiry KM, Abdallahi MO, Ba H, et al (2009) Preliminary study of malaria incidence in Nouakchott, Mauritania. Malar J 8:92

    Article  PubMed  PubMed Central  Google Scholar 

  16. Maltha J, Gillet P, Cnops L, et al (2010) Malaria rapid diagnostic tests: Plasmodium falciparum infections with high parasite densities may generate false positive Plasmodium vivax pLDH lines. Malar J 9:198

    Article  PubMed  PubMed Central  Google Scholar 

  17. Mappin B, Cameron E, Dalrymple U, et al (2015) Standardizing Plasmodium falciparum infection prevalence measured via microscopy versus rapid diagnostic test. Malar J 14:460

    Article  PubMed  PubMed Central  Google Scholar 

  18. Mendis K, Sina BJ, Marchesini P, Carter R (2001) The neglected burden of Plasmodium vivax malaria. Am J Trop Med Hyg 64(1-2 Suppl):97–106

    CAS  PubMed  Google Scholar 

  19. Ministère de la Santé de la République Islamique de Mauritanie (2006) Programme National de Lutte contre le Paludisme. Révision mars 2006

    Google Scholar 

  20. Ministère de la santé de la Mauritanie (2009) Programme National de Lutte contre le Paludisme. Plan stratégique de lutte contre le paludisme 2009–2015. 68 p

    Google Scholar 

  21. Munier A, Diallo A, Sokhna C, Chippaux JP (2009) Evaluation d’un test de diagnostic rapide du paludisme dans les postes de santé ruraux au Sénégal. Méd Trop 69(5):496–500

    CAS  Google Scholar 

  22. OMS (2009) Malaria Microscopy Quality Assurance Manual. Version 1, 146 p

    Google Scholar 

  23. OMS (2010) Guidelines for the treatment of malaria -2nd edition, 210 p

    Google Scholar 

  24. OMS (2012) Performance des tests de diagnostic rapide du paludisme. Bilan des résultats d’évaluation des produits par l’OMS: séries 1-4 (2008-2012), 17 p

    Google Scholar 

  25. OMS (2016) Paludisme Aide-mémoire N°94 Octobre [en ligne]

    Google Scholar 

  26. Ouattara A, Doumbo S, Saye R, et al (2011) Use of a pLDHbased dipstick in the diagnostic and therapeutic follow-up of malaria patients in Mali. Malar J 10:345

    Article  PubMed  PubMed Central  Google Scholar 

  27. Ould Abdallahi M, Alew I, Salem MS, et al (2014) Efficacy of artesunate-amodiaquine for the treatment of acute uncomplicated falciparum malaria in southern Mauritania. Malar J 13:496

    Article  Google Scholar 

  28. Ouldabdallahi M, Ouldbezeid M, Dieye M, et al (2011) Etude de la part du paludisme chez les consultants fébriles et des indices plasmodiques chez des écoliers dans la région du Trarza, République Islamique de Mauritanie. Bull Soc Pathol Exot 104(4):288–90 [http://www.pathexo.fr/documents/articles-bull/131490288.pdf]

    Article  CAS  PubMed  Google Scholar 

  29. Randrianasolo L, Tafangy BP, Raharimalala AL, et al (2007) Utilisation du test de diagnostic rapide du paludisme à Madagascar: étude préliminaire en 2003. Santé 17(2):69–73

    PubMed  Google Scholar 

  30. Salem MSA, Mohamed Lemine YO, Deida J, et al (2015). Efficacy of chloroquine for the treatment of Plasmodium vivax in the Saharan zone in Mauritania. Malar J 14:39

    Article  Google Scholar 

  31. Swarthout TD, Counihan H, Senga RK, van den Broek I (2007) Paracheck-Pf accuracy and recently treated Plasmodium falciparum infections: is there a risk of over-diagnosis? Malar J 6:58

    Article  PubMed  PubMed Central  Google Scholar 

  32. WHO (2015) World Malaria Report 2015, 280 p

    Google Scholar 

  33. Wurtz N, Lekweiry MK, Bogreau H, et al (2011) Vivax malaria in Mauritania includes infection of a Duffy-negative individual. Malar J 10:336

    Article  PubMed  PubMed Central  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institut national de recherche en santé publique (INRSP), BP 695, Nouakchott, Mauritanie

    H. Ba, Y. B. Deh, A. Tandia, M. Y. Diallo & M. B. Elkory

  2. Hôpital Le Dantec, Université Cheikh Anta Diop, Dakar, Sénégal

    A. D. Ahouidi & C. Diedhiou

  3. Department of Pathogen Molecular Biology, London School of Hygiene and Tropical Medicine, Keppel Street, London, WC1E 7HT, UK

    C. W. Duffy, S. Assefa & D. J. Conway

  4. Université des sciences et technologies médicales, Département de biologie, BP 880, Nouakchott, Mauritanie

    B. B. Lô

  5. Direction de l’inspection générale, av. Gamel Abdel, BP 115, Nouakchott, Mauritanie

    B. B. Lô

Authors
  1. H. Ba
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. D. Ahouidi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. C. W. Duffy
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Y. B. Deh
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. C. Diedhiou
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. A. Tandia
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. M. Y. Diallo
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. S. Assefa
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. B. B. Lô
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. M. B. Elkory
    View author publications

    You can also search for this author in PubMed Google Scholar

  11. D. J. Conway
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to H. Ba.

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Ba, H., Ahouidi, A.D., Duffy, C.W. et al. Evaluation du test de diagnostic rapide du paludisme OptiMal-IT® pLDH à la limite de la distribution de Plasmodium falciparum en Mauritanie. Bull. Soc. Pathol. Exot. 110, 31–37 (2017). https://doi.org/10.1007/s13149-017-0541-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13149-017-0541-y

Mots clés

Keywords

Search

Navigation