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Simulation of fractionated and continuous irradiation in photodynamic therapy: study the differences between photobleaching and singlet oxygen dose deposition

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Abstract

This study aims to compare the continuous irradiation with fractionated irradiation for photodynamic therapy (PDT) of solid tumors with intraperitoneally administered 5-aminolaevulinic acid (ALA). Therefore, considering the complex physiology of solid tumors and in order to inform simulations well, we did experiments on Balb/c mice using non-invasive fluorescence spectroscopy to have a feedback of protoporphyrin IX (PpIX) concentration in tumor just before irradiation and during treatment. PDT simulations were performed based on delivery of 36 J cm−2 total laser energy (630 nm) at the fluence rate of 40 mW cm−2 either for continuous or fractionated illumination. Based on the calculated amounts of 1O2 dose deposition and comparing these amounts with the 5 × 10 18 molecules cm−3 threshold of reacting 1O2, simulation results demonstrate that fractionated illumination with alternating light and dark periods of 60 s improved the tumor response further for PpIX-mediated PDT.

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Acknowledgments

This work was supported by grant from Tarbiat Modares University (TMU) 403/1514.

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Authors and Affiliations

  1. Department of Electrical Engineering, Faculty of Engineering, Ferdowsi University of Mashhad, Mashhad, Iran

    Nadia Naghavi

  2. Department of Biomedical Engineering, Faculty of Electrical & Computer Engineering, Tarbiat Modares University, Jalal Ale Ahmad Highway, P.O. Box: 14115-111, Tehran, Iran

    Mohammad Hossein Miranbaygi

  3. Medical Physics Research Center, Bu-Ali Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran

    Ameneh Sazgarnia

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  1. Nadia Naghavi
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  2. Mohammad Hossein Miranbaygi
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  3. Ameneh Sazgarnia
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Correspondence to Mohammad Hossein Miranbaygi.

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Naghavi, N., Miranbaygi, M.H. & Sazgarnia, A. Simulation of fractionated and continuous irradiation in photodynamic therapy: study the differences between photobleaching and singlet oxygen dose deposition. Australas Phys Eng Sci Med 34, 203–211 (2011). https://doi.org/10.1007/s13246-011-0064-6

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  • DOI: https://doi.org/10.1007/s13246-011-0064-6

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