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Initial evidence on differences among Enterovirus 71, Coxsackievirus A16 and Coxsackievirus B4 in binding to cell surface heparan sulphate

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Abstract

Cell surface heparan sulphate (HS) mediates infection for many viruses from diverse families. We demonstrated significant antiviral potencies for a number of HS mimetics against a cloned strain of Enterovirus 71 (EV71) in a previous study. Thus, the involvement of HS in mediating viral infection of isolates of human enteroviruses was investigated in Vero and human neural cells in the present work. In both cell lines, heparin and pentosan polysulphate significantly inhibited both infection and attachment of low passage clinical isolates of EV71 and Coxsackievirus A16 (CVA16) but showed no affect on Coxsackievirus B4 (CVB4) (p < 0.05). In addition, enzymatic removal of cell surface HS by heparinase I prevented binding of the clinical EV71 by nearly 50 % but failed to significantly inhibit CVA16 or CVB4 binding in Vero cells. Overall, the findings of this study provides evidence that whilst highly sulphated domains of HS serve as an essential attachment co-receptor for EV71, HS might be used as an alternative attachment receptor by the other member of Human Enterovirus group A, CVA16. In addition, HS may not mediate early infection in CVB4.

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Acknowledgments

This study was part of the PhD project of HRP at Swinburne University of Technology, Melbourne, Australia. The authors wish to thank Professor Peter C. McMinn (Central Clinical School, University of Sydney, Australia) for providing the cloned EV71 virus and the cells. The Australian Centre for infectious disease is also appreciated for providing the clinical isolates of EV71, CVA16 and CVB4.

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Authors and Affiliations

  1. Iranian Academic Centre for Education, Culture and Research (ACECR))-Mashhad Branch, P.O. Box 91775-1376, Mashhad, Iran

    Hamid Reza Pourianfar

  2. Environment and Biotechnology Centre, Swinburne University of Technology, Hawthorn, VIC, 3122, Australia

    Hamid Reza Pourianfar, Kristin Kirk & Lara Grollo

Authors
  1. Hamid Reza Pourianfar
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  2. Kristin Kirk
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  3. Lara Grollo
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Correspondence to Lara Grollo.

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Pourianfar, H.R., Kirk, K. & Grollo, L. Initial evidence on differences among Enterovirus 71, Coxsackievirus A16 and Coxsackievirus B4 in binding to cell surface heparan sulphate. VirusDis. 25, 277–284 (2014). https://doi.org/10.1007/s13337-013-0172-x

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  • DOI: https://doi.org/10.1007/s13337-013-0172-x

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