Abstract
Symptoms of little leaf, leaf chlorosis and leaf malformations with mosaic mottling symptoms were observed in two brinjal varieties (Pusa Shyamla and Pusa Purple Cluster) in fields of IARI, New Delhi, India during 2014–2015. Electron microscopy, PCR and sequence analysis first time provided evidence of association of Candidatus Phytoplasma trifolii with potato virus X and potato virus Y in brinjal in India.
Similar content being viewed by others
References
Azadvar M, Baranwal VK. Multilocus sequence analysis of phytoplasma associated with brinjal little leaf disease and its detection in Hishimonas phycitis in India. Phytopathog Mollicutes. 2012;2:15–21.
Bhat AI, Varma A, Pappu HR, Rajamannar M, Jain RK, Praveen S. Characterization of a potyvirus from eggplant (Solanum melongena) as a strain of potato virus Y by N-terminal serology and sequence relationships. Plant Pathol. 1999;48:648–54.
Gundersen DE, Lee IM. Ultrasensitive detection of phytoplasmas by nested-PCR assays using two universal primer pairs. Phytopathol Mediterr. 1996;35:144–51.
Kumar J, Gunapati S, Singh SP, Lalit A, Sharma NC, Tuli R. First report of a ‘Candidatus Phytoplasma asteris’ (16SrI group) associated with little leaf disease of Solanum melongena (brinjal) in India. New Dis Rep. 2012;26:21.
Mandal B, Kumar A, Rani P, Jain RK. Complete genome sequence, phylogenetic relationships and molecular diagnosis of an indian isolate of Potato Virus X. J Phytopathol. 2012;160:1–5.
Acknowledgments
The authors wish to express sincere thanks to Dr. Bikas Mandal for providing primer and laboratory facilities to carry out virus work. The authors are also thankful to the Head, Division of Plant Pathology and the Director, Indian Agricultural Research Institute for providing laboratory facilities.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Kumar, M., Katiyar, A., Madhupriya et al. First report of association of potato virus X and potato virus Y and ‘Candidatus Phytoplasma trifolii’ in brinjal in India. VirusDis. 27, 207–208 (2016). https://doi.org/10.1007/s13337-016-0318-8
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s13337-016-0318-8