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Establishment and partial characterization of a human tumor cell line, GBM-HSF, from a glioblastoma multiforme

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Abstract

This paper outlines the establishment of a new and stable cell line, designated GBM-HSF, from a malignant glioblastoma multiforme (GBM) removed from a 65-year-old Chinese woman. This cell line has been grown for 1 year without disruption and has been passaged over 50 times. The cells were adherently cultured in RPMI-1640 media with 10 % fetal bovine serum supplementation. Cells displayed spindle and polygonal morphology, and displayed multi-layered growth without evidence of contact inhibition. The cell line had a high growth rate with a doubling time of 51 h. The cells were able to grow without adhering to the culture plates, and 4.5 % of the total cells formed colonies in soft agar. The cell line has also been found to form tumors in nude mice and to be of a highly invasive nature. The cells were also partially characterized with RT-PCR. The RT-PCR revealed that Nestin, β-tubulin III, Map2, Klf4, Oct4, Sox2, Nanog, and CD26 were positively transcribed, whereas GFAP, Rex1, and CD133 were negatively transcribed in this cell line. These results suggest that the GBM-HSF cell line will provide a good model to study the properties of cancer stem cells and metastasis. It will also facilitate more detailed molecular and cellular studies of GBM cell division and pathology.

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Authors and Affiliations

  1. University of Science and Technology of China, Hefei, Anhui, 230026, People’s Republic of China

    Jiagui Qu

  2. State Key Laboratory of Brain and Cognitive Sciences, Kunming Institute of Zoology, Chinese Academy of Sciences, #32 Jiao Chang Dong Lu, Kunming, Yunnan, 650223, People’s Republic of China

    Jiagui Qu, Joshua D. Rizak, Tanzeel Huma & Yuanye Ma

  3. University of the Chinese Academy of Science, Beijing, 100101, People’s Republic of China

    Joshua D. Rizak

  4. Department of Neurosurgery, The Third Affiliated Hospital of Kunming Medical University (Yunnan Province Tumor Hospital), Kunming, Yunnan, 650118, People’s Republic of China

    Yaodong Fan

  5. Beijing Key Laboratory of Functional Food from Plant Resources, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, 100083, People’s Republic of China

    Xiaoxuan Guo

  6. CAS-Max Planck Junior Scientist Group, State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, 650223, People’s Republic of China

    Jiejing Li

  7. Reproductive Neuroendocrinology Laboratory, Department of Animal Sciences, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, 45320, Pakistan

    Tanzeel Huma

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  1. Jiagui Qu
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Correspondence to Yuanye Ma.

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Qu, J., Rizak, J.D., Fan, Y. et al. Establishment and partial characterization of a human tumor cell line, GBM-HSF, from a glioblastoma multiforme. Human Cell 27, 129–136 (2014). https://doi.org/10.1007/s13577-013-0086-3

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  • DOI: https://doi.org/10.1007/s13577-013-0086-3

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