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MicroRNA-30e inhibits proliferation and invasion of non-small cell lung cancer via targeting SOX9

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Abstract

Previous studies have reported that microRNA-30e (miR-30e) is dysregulated in multiple human cancers. However, the expression, functions and molecular mechanism of miR-30e in NSCLC remain unknown. In this study, we found that miR-30e was expressed at a low level in NSCLC tissues and cell lines. In NSCLC cell lines, enforced expression of miR-30e could inhibit cell proliferation and invasion in vitro. In addition, miR-30e negatively regulated SOX9 expression through directly binding to the 3′UTR of SOX9, and an inverse correlation was found between miR-30e and SOX9 mRNA expression in NSCLC tissues. Moreover, knockdown of SOX9 led to decreased proliferation and invasion of NSCLC cells. Taken together, miR-30e acts as a tumor suppressor in NSCLC, and inhibits cell proliferation and invasion possibly by directly targeting SOX9. These findings might provide novel therapeutic targets for NSCLC.

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Acknowledgements

This work was supported by a grant from the National Nature Science Foundation of China (181774078, 81773453, 81803886), National Nature Science Foundation of Liaoning Province, China (201602227, 20170540300).

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Author notes

  1. Yanwei Cui and Lei Zhao contributed equally to this work and should be considered co-first author.

Authors and Affiliations

  1. Department of Thoracic Surgery, The First Affiliated Hospital of Dalian Medical University, Zhongshan Road # 222, Dalian, 116011, China

    Yanwei Cui, Lei Zhao, Shilei Zhao, Tao Guo, Fengzhou Li, Zhuoshi Li, Lei Fang & Chundong Gu

  2. Lung Cancer Diagnosis and Treatment Center of Dalian, The First Affiliated Hospital of Dalian Medical University, Zhongshan Road # 222, Dalian, 116011, China

    Yanwei Cui, Lei Zhao, Shilei Zhao, Tao Guo, Fengzhou Li, Zhuoshi Li, Lei Fang & Chundong Gu

  3. Departments of Respiratory Medicine, The First Affiliated Hospital of Dalian Medical University, Zhongshan Road # 222, Dalian, 116011, China

    Taihua Wu

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  1. Yanwei Cui
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Correspondence to Taihua Wu or Chundong Gu.

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Cui, Y., Zhao, L., Zhao, S. et al. MicroRNA-30e inhibits proliferation and invasion of non-small cell lung cancer via targeting SOX9. Human Cell 32, 326–333 (2019). https://doi.org/10.1007/s13577-018-0223-0

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