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Analgesic and antioxidant activities of Colocasia fallax

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Abstract

The ethanol extract of tuber part of Colocasia fallax Schott (Araceae) was tested for its potential analgesic and antioxidant activities. Acetic acid induced writhing method was used to evaluate the analgesic activity at the concentration of 250 and 500 mg/kg body weight. In order to investigate the antioxidant activity, four complementary test systems, namely, 2,2-diphenyl-1-picryl-hydrazil (DPPH) free radical scavenging, ferric reducing power assay, total phenolic and flavonoid contents were used. Polyphenolic compounds were also detected by HPLC analysis. The extract showed significant (P < 0.005) analgesic activity with 32.14 and 53.57 % writhing inhibition at the concentrations of 250 and 500 mg/kg body weight, respectively that was comparable to standard diclofenac sodium (78.57 % at 25 mg/kg). In DPPH radical scavenging assay, the extract showed the IC50 value of 78.94 μg/ml. Reducing power of the extract was comparable to that of butylated hydroxy toluene (BHT). In HPLC analysis of polyphenols, epicatechin, myricetin, trans-cinnamic, syringic acid, vanillin, p-coumaric acid, trans-ferulic acid and rosmarinic acid were detected in the extract (95.95, 52.14, 20.39, 8.32, 8.43, 6.36, 4.18 and 6.73 mg/100 g of dry extract, respectively). Presence of polyphenols in the extract might have attributed towards the observed bio-activity. Present finding also suggests the traditional use of this plant in the management of pain.

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References

  • Allen DJ (2011) Colocasiafallax. The International Union for Conservation of Nature and Natural Resources (IUCN) Red List of Threatened Species. Version 2015.2

  • Areekul V, Phomkaivon N (2015) Thai indigenous plants: focusing on Total phenolic content, antioxidant activity and their correlation on medicinal effects. KMITL Sci Tech J 15(1)

  • Atoui AK, Mansouri A, Boskou G, Kefalas P ( 2005) Tea and herbal infusions: their antioxidant activity and phenolic profile. Food Chem 89:27–36

  • Brukil H (1966) A Dictionary of the economic products of malay peninsula, Governments of Malaysia & Singapour by ministry of Agriculture and cooparetives; kualalampur, Malaysia. Vol-I (A-H) and vol-II(I-Z); p 2444

  • Dehpour AA, Ebrahimzadeh MA, Nabavi SF, Nabavi SM (2009) Antioxidant activity of methanol extract of Ferula asafoetida and its essential oil composition. Grasas Aceites 60(4):405–412

    Article  CAS  Google Scholar 

  • Duh PD, Tu YY, Yen GC (1999) Antioxidant activity of the aqueous extract of harng Jyur (Chrysanthemum morifolium Ramat). Lebensm Wiss Technol 32:269–277

    Article  CAS  Google Scholar 

  • Emira N, Mejdi S, Inès N, Eulogio V, Mahjoub A, Abdulbasit A (2014) Comparative study on the antifungal and antioxidant properties of natural and colored Juglansregia L. barks: A high activity against vaginal Candida strains. Life Sci J 11(8):327–335

    Google Scholar 

  • Ghani A (2005) Practical phytochemistry. Parash Publishers, Dhaka, pp. 8–20

    Google Scholar 

  • Guan-Jhong H, Ming-Hsing H, Chuan-Sung C, Shyh-Shyun H, Pei-Hsin S, Ming-Tsung Y, Bor-Sen W (2012) Analgesic and anti-inflammatory activities of aqueous extracts of Fructus LigustriLucidi. J Food Drug Anal 20(3):617–627

    Google Scholar 

  • Gülçin I, Küfrevio I, Oktay M, Büyükokuro ME (2004) Antioxidant, antimicrobial, antiulcer and analgesic activities of nettle (Urtica dioica L.). J Ethnopharmacol 90:205–215

    Article  PubMed  Google Scholar 

  • Harleen KS, Bimlesh K, Sunil P, Prashant T, Manoj S, Pardeep S (2011) A review of phytochemistry and pharmacology of flavonoids. Int Pharm Sci 1(1):25–41

    Google Scholar 

  • Hemayet H, Ismet AJ, Sariful IH, Jamil AS, Shubhra KD, Arpona H, Arif A (2013) Anti- inflammatory and antioxidant activities of ethanolic leaf extract of Brownlowiatersa (L.) Kosterm. Orient Pharm Exp Med 13(3):181–189

    Article  Google Scholar 

  • Himangsu M, Sanjib S, Khalijah A, Hemayet H, Abdulwali A, Khirul MI, Ismet AJ, Samir KS, Golam MH, Jamil AS, Shaikh JU (2014) Central-stimulating and analgesic activity of the ethanolic extract of Alternantherasessilis in mice. BMC Complement Altern Med 14:398

    Article  Google Scholar 

  • Hosne A, Abul MH (2006) Three new records of Aroids (Araceae) for Bangladesh. Bangladesh J Plant Taxon 13(2):83–91

    Google Scholar 

  • Hoult JRS, Moroney MA, Paya M (1994) Actions of flavonoids and coumarins on lipoxygenase and cyclooxygenase. Methods Enzymol 234:443–454

    Article  CAS  PubMed  Google Scholar 

  • Inmaculada N, Rocío G, Verónica G, Ana Belén B, María JP (2015) Nutritional composition and antioxidant capacity in edible flowers: characterisation of phenolic compounds by HPLC-DAD ESI/MSn. Int J Mol Sci 16:805–822

    Google Scholar 

  • Islam MA, Ahmed F, Das AK, Bachar SC (2005) Analgesic and anti-inflammatory activity of Leonurus sibiricus. Fitoterapia 76:359–362

    Article  PubMed  Google Scholar 

  • Islam MK, Nripendra NB, Sanjib S, Hemayet H, Ismet AJ, Tanzir AK, Khalijah A, Jamil AS (2014) Antinociceptive and antioxidant activity of Zanthoxylumbudrunga Wall (Rutaceae) seeds. Sci World J. doi:10.1155/2014/869537

    Google Scholar 

  • Ismet AJ, Proity NA, Nasima K, Tanzir AK, Mohammad MR, Arpona H, Hemayet H (2014) Comparative study of anti-nociceptive activity and phenolic content of the ethanol extracts of Piper nigrum and Piper longum fruits. Int J Pharm Sci Rev Res 27(1):47–52

    Google Scholar 

  • Koster R, Anderson M, De-Beer EJ (1959) Acetic acid for analgesic screening. Fed Proc 18:412–418

    Google Scholar 

  • Kulisica T, Radonicb A, Katalinicc V, Milosa M (2004) Use of different methods for testing antioxidative activity of oregano essential oil. Food Chem 85:633–640

  • Kumar S, Pandey AK (2013) Chemistry and biological activities of flavonoids: An overview. ScientificWorldJournal. doi:10.1155/2013/162750

    Google Scholar 

  • Miliauskas G, Venskutonis PR, Van Beek TA (2004) Screening of radical scavenging activity of some medicinal plants and aromatic plant extract. Food Chem 85:231–237

  • Monika M, Nomita G, Palak P, Varsha M, Manisha K (2014) Phytochemical evaluation and pharmacological activity of Syzygium aromaticum: a comprehensive review. Int J Pharm Pharm Sci 6(8):67–72

    Google Scholar 

  • Oyaizu M (1986) Studies on product of browning reaction prepared from glucosamine. Jpn J Nutr Diet 44(6):307–315

    Article  CAS  Google Scholar 

  • Rates SMK (2001) Review: plants as source of drugs. Toxicon 39:603–613

    Article  CAS  PubMed  Google Scholar 

  • Rodriguez EB, Flavier ME, Rodriguez-Amaya DB, Amaya-Farfán J (2006) Phytochemicals and functional foods. current situation and prospect for developing countries. Segurança Alimentar e Nutricional, Campinas 13(1):1–22

    Google Scholar 

  • Saha S, Shilpi JA, Mondal H, Gofur R, Billah M, Nahar L, Satyajit DS (2013) Bioactivity studies on Musa seminifera Lour. Pharmacogn Mag 9(36):315–322

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Shah M, Behara YR, Jagadeesh B (2012) Phytochemical screening and in vitro antioxidant activity of aqueous and hydroalcoholic extract of Bacopamonnieri Linn. Int J Pharm Sci Res 3(9):3418–3424

    Google Scholar 

  • Sharma V, Singh M (2014) Antinephrotoxic efficacy of Operculina turpethum and its isolated stigma-5,22 dien-3-O-b-D-glucopyranoside against N-nitrosodimethylamine induced renal carcinogenesis in male mice. Int J Phytopharm 4(2):777779–777756

    Google Scholar 

  • Shilpi JA, Rouf R, Ferdous MM, Uddin SJ (2006) Central nervous system stimulatingactivity of the ethanolic extract of Fleurya interrupta Gaud.(Urticaceae). Orient Pharm Exp Med 6(1):21–26

    Article  Google Scholar 

  • Sina C, Ion T, Violeta N, Mira I, Felicia T (2014) Phenolics content, antioxidant activity and color of green walnut extracts for preparing walnut liquor. Not Bot Horti Agrobo 42(2):551–555

    Google Scholar 

  • Tanaka M, Kuie CW, Nagashima Y, Taguchi T (1988) Applications of antioxidative Maillard reaction products from histidine and glucose to sardine products. Nippon Suisan Gakk 54:1409–14

  • Uddin SJ, Shilpi JA, Delazar A, Nahar L, Sarker SD (2004) Free radical scavenging activity of some Bangladeshi plant extracts. Orient Pharm Exp Med 4(3):187–195

    Article  Google Scholar 

  • Williams DJ, Edwards D, Hamernigb I, Jian L, James AP, Johnson SK, Tapsell LC (2013) Vegetables containing phytochemicals with potential anti-obesity properties: a review. Food Res Int 52(1):323–333

    Article  CAS  Google Scholar 

  • Yen GC, Duh PD (1994) Scavenging Effect of Methanolic Extracts of Peanut Hulls on Free-Radical and Active-Oxygen Species. J Agric Food Chem 42(3):629–632

Download references

Acknowledgments

Authors are grateful to Pharmacy Discipline, Life Science School, Khulna University, Khulna, Bangladesh for providing technical support to carry out the present investigations successfully. We would like thanks Mr. Hemayet Hossain, Scientific Officer, BCSIR, Dhaka, Bangladesh for HPLC analysis of the sample.

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Authors and Affiliations

  1. Pharmacy Discipline, Life Science School, Khulna University, Khulna, 9208, Bangladesh

    Md. Nazmul Hasan Zilani, Md. Amirul Islam, Jamil Ahmad Shilpi, Md. Mustafizur Rahman & Md. Golam Hossain

  2. Fisheries and Marine Resource Technology Discipline, Life Science School, Khulna University, Khulna, 9208, Bangladesh

    Sharmin Sultana Khushi

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  1. Md. Nazmul Hasan Zilani
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  2. Md. Amirul Islam
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  3. Sharmin Sultana Khushi
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  4. Jamil Ahmad Shilpi
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  5. Md. Mustafizur Rahman
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  6. Md. Golam Hossain
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Correspondence to Md. Amirul Islam.

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Zilani, M.N.H., Islam, M.A., Khushi, S.S. et al. Analgesic and antioxidant activities of Colocasia fallax . Orient Pharm Exp Med 16, 131–137 (2016). https://doi.org/10.1007/s13596-016-0222-1

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