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Conventional and experimental treatments for pit and fissure sealing associated with ND:YAG laser

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Abstract

Objective

The aim of this study was to evaluate the efficacy of different treatments for sealing pits and fissures (FF) on enamel surface against artificial caries lesions.

Material and methods

Thirty sound human premolar crowns were randomly divided into six groups (n = 5), according to FF treatment: C, control (no treatment); L, irradiation with Nd:YAG laser (80 mJ/10 Hz/1064 nm/60 s); F, FluroShield (Dentsply); F+L, FluroShield + Nd:YAG laser; HA+A, hydroxyapatite powder bonded to the adhesive system (Universal Single Bond, 3M ESPE); and HA+A+L, hydroxyapatite mixing associated with laser irradiation. After treatment, the samples were immersed in artificial caries solution for 14 days. Occlusal surfaces were evaluated by laser fluorescence before and after treatments in order to confirm caries formation. Samples were sectioned longitudinally to prepare slides for polarized light microscopy evaluation, to provide three measurements of the depth of caries cavities formed. Data were submitted to one-way ANOVA, Tukey tests (p < 0.05), and Dunnett’s test to compare variables.

Results

ANOVA showed statistically significant differences among groups (p = 0.04). Group L differed significantly from group C. The other treatments showed no statistically significant differences between groups.

Conclusion

Treatment with Nd:YAG laser presented greater pit and fissure sealing of incipient caries lesions.

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Author information

Authors and Affiliations

  1. Department of Restorative Dentistry, Institute of Science and Technology, Campus São José dos Campos, Sao Paulo State University - (UNESP), Av. Engenheiro Francisco José Longo, 777, Jardim São Dimas, São José dos Campos, SP, Brazil

    F.A Feitosa, E Crastechini, S.R.M.S Esteves, T.M Silva, M.F.R.L Huhtala & S.E.P Gonçalves

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  1. F.A Feitosa
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  2. E Crastechini
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  3. S.R.M.S Esteves
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  4. T.M Silva
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  5. M.F.R.L Huhtala
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  6. S.E.P Gonçalves
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Corresponding author

Correspondence to T.M Silva.

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The authors declare that they have no conflict of interest.

Ethical approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards.

Informed consent

Thirty sound human premolars that were extracted for therapeutic reasons under approval of the Research Ethical Committee at the São José dos Campos School of Dentistry were used in this study. Informed consent was obtained from all individual participants included in the study.

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Feitosa, F., Crastechini, E., Esteves, S. et al. Conventional and experimental treatments for pit and fissure sealing associated with ND:YAG laser. Laser Dent Sci 1, 33–40 (2017). https://doi.org/10.1007/s41547-017-0004-1

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  • DOI: https://doi.org/10.1007/s41547-017-0004-1

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