Abstract
A tomato yellow leaf curl geminivirus (TYLCV-AL), was first identified in tomato plants in Almeria, southern Spain in 1992. This virus is transmitted by the tobacco whitefly, Bemisia tabaci (Gennadius), and is presently infecting tomato crops throughout the south eastern region of Spain. Solanum nigrum, collected from a field in south east Spain and exhibiting leaf curl symptoms, was squash blotted onto nylon membrane and gave a positive signal when hybridised to a TYLCV-Is DNA probe. Laboratory tests showed B. tabaci to transmit TYLCV-AL from infected tomato plants to S. nigrum seedlings. The virus could then be acquired by B. tabaci and transmitted back from infected S. nigrum plants to tomato, inducing typical TYLCV symptoms. These results indicate the importance of S. nigrum as a weed host/reservoir for a TYLCV and its possible role in the spread of this virus within Europe.
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References
Bedford ID, Briddon RW, Brown JK, Rosell RC and Markham PG (1994a) Geminivirus transmission and biological characterisation ofBemisia tabaci(Gennadius) biotypes fromdifferent geographic regions. Annals of Applied Biology 125: 311-325
Bedford ID, Briddon RW, Markham PG, Rosell RC and Brown JK (1993) A new species of Bemisiaor biotype of Bemisia tabacias a future pest of European agriculture. In: Plant Health and the European Single Market, D. Ebbles (ed), BCPC Monograph No. 54: 381-386
Bedford ID, Pinner M, Liu S and Markham PG (1994b) Bemisia tabaci- Potential infestation, phytotoxicity and virus transmission within European Agriculture. Brighton Crop Protection Conference - Pests and Diseases 1994, 3: 911-916
Caciagli P, Bosco D and Al-Bitar L (1995) Relationships of the Sardinian isolate of tomato yellow leaf curl geminivirus with its whitefly vector Bemisia tabaciGen. European Journal of Plant Pathology 101: 163-170
Crespi S, Noris E, Vaira A and Accotto GP (1995) Molecular characterisation of cloned DNA from a tomato yellow leaf curl isolate from Sicily. Phytopathologia Mediterranea. 34: 93-99
Czosnek H, Ber R, Antignus Y, Cohen S, Navot N and Zamir D (1988) Isolation of tomato yellow leaf curl virus, a geminivirus. Phytopathology 78: 508-512
Louro D, Noris E, Verrati F and Accotto GP (1996) First report of Tomato Yellow Leaf Curl Virus in Portugal. Plant Disease 80: 1079
Markham PG, Bedford ID, Liu S and Pinner MS (1994) The transmission of geminiviruses by Bemisia tabaci. Pesticide Science 42: 123-128
Maule AJ, Hull R, Donson J (1983) The application of spot hybridisation to the detection of DNA and RNA viruses in plant tissues. Journal of Virological Methods 6: 215-224
Moriones E, Arno J, Accotto GP, Noris E and Cavallarin L (1993) First report of Tomato Yellow Leaf Curl Virus in Spain. Plant Disease 9: 953
Noris E, Hidalgo E, Accotto GP and Moriones E (1994) High similarity among the TYLCV isolates from the west Mediterranean basin: The nucleotide sequence of an infectious clone from Spain. Archives of Virology 135: 165-170
Rigby PWJ, Dieckmann M, Rhodes C, Berg P (1977) Labelling deoxyribonucleic acid to a high specific activity in vitroby nick translation with DNA polymerase. Journal of Molecular Biology 113: 237-251
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Bedford, I., Kelly, A., Banks, G. et al. Solanum nigrum: an indigenous weed reservoir for a tomato yellow leaf curl geminivirus in southern Spain. European Journal of Plant Pathology 104, 221–222 (1998). https://doi.org/10.1023/A:1008627419450
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DOI: https://doi.org/10.1023/A:1008627419450