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Reversibility of Cisternal Stack Formation During Hypoxic Hypoxia and Subsequent Reoxygenation in Cerebellar Purkinje Cells

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Abstract

Cisternal stacks are induced during hypoxia, which may be associated with intracellular Ca2+ regulation. Although neurons are divided internally in different compartments, little is known about regional differences in cisternal stack formation. We investigated the effects of hypoxic hypoxia and later reoxygenation on cisternal stack formation and other ultrastructual changes in the proximal dendrite, dendritic spine, and cell body of cerebellar Purkinje cells in rats. After brief hypoxic events, cisternal stacks appeared predominantly in the proximal dendrites and after longer hypoxic events in dendritic spines and cell body. Following reoxygenation, cisternal stacks disappeared first in the cell body, followed by the dendritic spines, then the proximal dendrites. These results showed that stack formation occurred at different degrees and time courses among the three regions, and the effect was reversible, which suggests that these compartments are differentially sensitive to hypoxia.

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Authors and Affiliations

  1. Department of Anesthesiology, National Defense Medical College, Tokorozawa, Saitama, Japan

    Takeshi Ikemoto & Tetsuo Satoh

  2. Department of Pharmacology, Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest, Hungary

    Takeshi Ikemoto & E. Sylvester Vizi

  3. Department of Anatomy II, Gunma University School of Medicine, Maebashi, Gunma, Japan

    Hiroshi Yorifuji

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  1. Takeshi Ikemoto
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  2. Hiroshi Yorifuji
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  4. E. Sylvester Vizi
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Correspondence to Hiroshi Yorifuji.

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Ikemoto, T., Yorifuji, H., Satoh, T. et al. Reversibility of Cisternal Stack Formation During Hypoxic Hypoxia and Subsequent Reoxygenation in Cerebellar Purkinje Cells. Neurochem Res 28, 1535–1542 (2003). https://doi.org/10.1023/A:1025674409572

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