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Molecular Typing of a BHV-4 (Bovine herpesvirus 4) Field Isolate

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Abstract

A new BHV-4 (bovine herpesvirus 4) isolated from a case of bovine interdigital dermatitis was characterized by PCR and restriction enzyme analysis. To determine whether the new isolate (PR/1) belonged to BHV-4, DNA from infected cells was specifically amplified by PCR. We used a set of primers spanning a large 2.571 kb conserved region of the BHV-4 genome, including the 3′ end of ORF1 (homologous to the EBV BVRF1 gene), ORF2 (homologous to the EBV BXRF1 gene), ORF3 (TK gene) and ORF4 (gH gene) 5′ end, respectively. The identity of the amplified product was confirmed by HindIII restriction enzyme digestion and Southern hybridization. No product was observed from the DNA of other bovine herpesviruses tested. The restriction patterns of the PR/1 genome compared to DN 599, MOVAR 33/63 and LVR BHV-4 reference strains showed two kinds of differences, either related or not related to the prDNA (polyrepetitive DNA). Taken together, these data show that PR/1 is a new BHV-4. We would consider that the present report provides a scheme of work for diagnosis and typing of BHV-4 isolates.

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REFERENCES

  • Bartha, A., Juhasz, M. and Liebermann, H., 1966. Isolation of a bovine herpesvirus from calves with respiratory disease and keratoconjunctivitis. Acta Veterinaria Academiae Scientarum Hungaricae, 16, 357-358

    Google Scholar 

  • Bublot, M., Wellemans, G., Van Bressem, M.F., Dubuisson, J., Pastoret, P.P. and Thiry, E., 1991. Genomic diversity among bovine herpesvirus 4 field isolates. Archives of Virology, 116, 1-18

    Google Scholar 

  • Bublot, M., Lomonte, P., Lequarre, A.S., Albrecht, J.C., Nicholas, J., Fleckenstein, B., Pastoret, P.P. and Thiry, E., 1992. Genetic relationship between bovine herpesvirus 4 and the gammaherpesviruses Epstein–Barr virus and herpesvirus saimiri. Virology, 190, 654-655

    Google Scholar 

  • Castrucci, G., Frigeri, F., Ferrari, M., Ranucci, S., Aldrovandi, V., Cilli, V., Rampichini, L. and Gatti, R., 1987. Experimental infection of calves with strain of bovid herpesvirus-4. Comparative Immunology, Microbiology and Infectious Disease, 10, 41-49

    Google Scholar 

  • Cavirani, S., Martelli, P., Cabassi, C.S., Lavazza, A., Allegri, G. and Flammini, C.F., 1996. Isolation of bovine herpesvirus 4 (BHV-4) from dairy cows with digital dermatitis. Proceedings of the XIX World Buiatric Congress, Edinburgh, 121

  • Dubuisson, J., Thiry, E., Thalasso, F., Bublot, M. and Pastoret, P.P., 1988. Biological and biochemical comparison of bovid herpesvirus-4 strains. Veterinary Microbiology, 16, 339-349

    Google Scholar 

  • Ehlers, B., Buhk, H.J. and Ludwig, H., 1985. Analysis of bovine cytomegalovirus genome structure: cloning and mapping of the monomeric polyrepetitive DNA unit, and comparison of European and American strains. Journal of General Virology, 66, 55-68

    Google Scholar 

  • Grassman, R. and Fleckenstein, B., 1989. Recombinant herpesvirus saimiri is capable of persisting in a human T-cell line. Journal of Virology, 63, 1818-1821

    Google Scholar 

  • Guo, Z.W., Shen, D.T., Evermann, F.J. and Gorham, J.R., 1988. Comparison of an enzyme-linked immunosorbent assay and complement-fixation test for the detection of IgG to bovine herpesvirus type 4 (bovine cytomegalovirus). American Journal of Veterinary Research, 49, 667-670

    Google Scholar 

  • Innis, A.M., Gelfand, H.D., Sninky, J.J. and White, T.J., 1990. PCR Protocols: A Guide to Methods and Applications, (Academic Press, New York)

    Google Scholar 

  • Kit, S., Kit, M., Ichimura, H., Crandel, R. and McConnel, S., 1986. Induction of thymidine kinase activity by viruses with group B DNA genomes: bovine cytomegalovirus (bovine herpes virus 4). Virus Research, 4, 197-212

    Google Scholar 

  • Lavazza, A., Pascucci, S. and Gelmetti, D., 1990. Rod-shaped virus-like particles in intestinal contents of three avian species. The Veterinary Record, 23, 581

    Google Scholar 

  • Lin, T.M., Shi, G.Y., Tsai, C.F., Janny Su, H., Leon Guo, Y.L. and Wu, H.L., 1997. Susceptibility of endothelial cells to bovine herpesvirus type 4 (BHV-4). Journal of Virological Methods, 63, 219-225

    Google Scholar 

  • Lomonte, P., Bublot, M., Pastoret, P. and Thiry, E., 1992. Location and characterization of the bovine herpes virus type 4 thymidine kinase gene; comparison with thymidine kinase genes of other herpesviruses. Archives of Virology, 127, 327-337

    Google Scholar 

  • Ludwig, H., 1983. Bovine herpesviruses. In: B. Roizman (ed.), The Herpesviruses, vol. 2, (Plenum Press, New York), 135-214

    Google Scholar 

  • Maniatis, T., Fritsch, E.F. and Sambrook, J., 1989. Molecular Cloning: A Laboratory Manual, (Cold Spring Harbor Laboratory Press, New York)

    Google Scholar 

  • Mohanty, S.B., Hammond, R.C. and Lillie, M.G., 1971. A new bovine herpesvirus and its effect on experimentally infected calves. Archiv für die Gesamte Virusforschung, 34, 394-395

    Google Scholar 

  • Osorio, F.A., Reed, D.E., Van DerMaaten, M.J. and Metz, C.A., 1985. Comparison of the herpesviruses of cattle by restriction endonuclease analysis and serologic analysis. American Journal of Veterinary Research, 46, 2104-2109

    Google Scholar 

  • Rovnak, J., Quackenbush, S.L., Reyes, R.A., Baines, J.D., Parrish, C.R. and Casey, J.W., 1998. Detection of a novel bovine lymphotropic herpesvirus. Journal of Virology, 72, 4337-4242

    Google Scholar 

  • Schulz, T.F., 1998. Kaposi's sarcoma-associated herpesvirus (human herpesvirus-8). Journal of General Virology, 79, 1573-1591

    Google Scholar 

  • Storz, J., Ehlers, B., Todd, V.J. and Ludwig, H., 1984. Bovine cytomegalovirus: identification and differential properties. Journal of General Virology, 65, 697-706

    Google Scholar 

  • Thiry, E., Bublot, M., Dubuisson, J. and Pastoret, P.P., 1989. Bovine herpesvirus-4 (BHV-4) infection in cattle. In: G. Wittmann (ed.), Herpesvirus Diseases of Cattle, Horses and Pigs, (Kluwer, Boston, MA), 96-115

    Google Scholar 

  • Van Opdenbosh, E., Wellemans, G. and Oudewater, J., 1986. Casual isolation of a bovine herpesvirus 4 from the lung of a sheep. Vlaams Diergeneeskundig Tijdschrift, 55, 432-433

    Google Scholar 

  • Wellemans, G., Van Opdenbosh, E. and Mammerickx, M., 1986. Inoculation expérimental du virus LVR 140 (herpes bovin IV) à des vaches gestantes et non-gestantes. Annales des Recherches Véterinaires, 17, 89-94

    Google Scholar 

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Authors and Affiliations

  1. Istituto di Malattie Infettive Profilassi e Polizia Veterinaria, Facoltà di Medicina Veterinaria, Università di Parma, 43100, Parma, Italy

    G. Donofrio, S. Cavirani, C.F. Flammini & F. Scatozza

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  1. G. Donofrio
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  2. S. Cavirani
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  3. C.F. Flammini
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  4. F. Scatozza
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Donofrio, G., Cavirani, S., Flammini, C. et al. Molecular Typing of a BHV-4 (Bovine herpesvirus 4) Field Isolate. Vet Res Commun 24, 411–422 (2000). https://doi.org/10.1023/A:1006478317972

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